Authors: X.B. Wang, Z.J. Liu, Y.J. Lv, Y. Long and E.D. Bao
In this study, the immune response induced by a mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) was evaluated in chickens inoculated with infectious bursal disease virus (IBDV) vaccine. After the mixture was injected intramuscularly at a dose of 0.075, 0.15 or 0.3 mg·kg-1·.. Read More»
Authors: S.C.C. Silva, E. Gasparino, D.M. Voltolini, A.V. Pires, F. Tanamati, A.L.S. Garcia and D.P. de Oliveira
We evaluated messenger RNA (mRNA) expression of the growth-hormone (GHR) and insulin-like growth factor (IGF-1) genes in 28-day-old Japanese meat quails fed diets containing 0, 8, or 12% dietary glycerol in substitution of corn. Total RNA was extracted from the breast muscle and the DNA was amplified with specific primers using real-time PCR. Feed convers.. Read More»
Authors: Y.J. Liu and J.Y. Zhang
With advances in molecular biology, microarray data have become an important resource in the exploration of complex human diseases. Although gene chip technology continues to grow, there are still many barriers to overcome, such as high costs, small sample sizes, complex procedures, poor repeatability, and the dependence on da.. Read More»
Authors: X.J. Wei, S.Q. Tang, G.N. Shao, M.L. Chen, Y.C. Hu and P.S. Hu
Plant height is one of the most important agronomic traits of rice (Oryza sativa). Dwarf mutants are ideal materials for research on the mechanisms of regulation of rice plant height. We examined a new dwarf and narrow-leaf mutant dnl1. Phenotypic analysis showed that the dnl1 mutant has a thinner culm and more tillers, but the number of grains per panicl.. Read More»
Authors: H.M. Hou, H.E. Li, M. Gao, H. Wang, C. Jiao and X.P. Wang
Using rapid amplification of cDNA ends, a full-length cDNA sequence of a GDP-L-galactose phosphorylase-like gene was isolated from leaves infected by Erysiphe necator in the Chinese wild (Vitis pseudoreticulata) clone, ‘Baihe-35-1’, an E. necator-resistant genotype. The full-length cDNA, designated as VpVTC, comprised 1943 bp and putatively en.. Read More»
Authors: B. Yang, J.R. Xu, X.M. Liu, Y. Yang, X.F. Na, M. Li and Y.J. Wang
We investigated a possible association of polymorphism of the eNOS gene and essential hypertension in the Chinese Hui population; polymorphisms of rs2070744 (T>C), rs1799983 (G>T), rs1800780 (A>G), and rs3918181 (A>G) loci of the eNOS gene were examined. We found that the genotypic frequencies at rs1799983 and rs1800780 loci differed significa.. Read More»
Authors: L.J. Cui, X.Y. Chang, L.Y. Zhu, G. Feng, T. Zhou, C.X. Zhang, K.Y. Chong and K. Sun
Recently genome-wide association studies on East Asian populations reported an association between diabetes and several single nucleotide polymorphisms (SNPs) in a 40-kb linkage disequilibrium block in intron 15 of KCNQ1. However, the association between KCNQ1 variants and type 2 diabetes mellitus (T2DM) in Chinese Kazakh popu.. Read More»
Authors: R.R. Aguiar, L.A. Ambrosio, G. SepĂ?Âșlveda-Hermosilla, V. Maracaja-Coutinho and A.R. Paschoal
This report describes the miRQuest - a novel middleware available in a Web server that allows the end user to do the miRNA research in a user-friendly way. It is known that there are many prediction tools for microRNA (miRNA) identification that use different programming languages and methods to realize this task. It is diffic.. Read More»
Authors: P.C. Guo1*, S.Q. Yan1*, S. Si1, C.Y. Bai1, Y. Zhao1, Y. Zhang1, J.Y. Yao2 and Y.M. Li1
The blue fox (Alopex lagopus), a coat-color variant of the Arctic fox, is a domesticated fur-bearing mammal. In the present study, transcriptome data generated from a pool of nine different tissues were obtained with Illumina HiSeq2500 paired-end sequencing technology. After filtering from raw reads, 32,358,290 clean reads wer.. Read More»
Authors: Y.X. Jing, L.N. Wang, X.M. Wu and C.X. Song
Ebola hemorrhagic fever is a fatal disease caused by the negative-strand RNA of the Ebola virus. A high-intensity outbreak of this fever was reported in West Africa last year; however, there is currently no definitive treatment strategy available for this disease. In this study, we analyzed the molecular evolutionary history a.. Read More»