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Animal Genetics   Research Article

Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing

Authors: X. Qiao, J.H. Wu,, R.B. W, R. Su, C. Li, Y.J. Zhang1, R.J. Wang, Y.H. Zhao, Y.X. Fan, W.G. Zhang and J.Q. Li

The mammalian hair follicle (HF) is a unique, highly regenerative organ with a distinct developmental cycle. Cashmere goatCapra hircus) HFs can be divided into two categories based on structure and development time: primary and secondary follicles. To identify differentially expressed genes (DEGs) in the primary and secondary .. Read More»

Genet. Mol. Res. 15(3):
gmr.15038589
DOI:
10.4238/gmr.15038589
Bioinformatics   Research Article

Use of microsatellite markers in molecular analysis of segregating populations of papaya (Carica papaya L.) derived from backcrossing

Authors: F.O. Pinto, M.G. Pereira, L.N. Luz, D.L. Cardozo, H.C.C. Ramos and C.M.P. Macedo

Brazil is the world leader in papaya production. However, only a small number of cultivars are registered for commercial planting, mainly owing to delays in obtaining cultivars and the high costs of the field phase of breeding programs. These costs can be reduced when molecular tools are combined with conventional breeding methods. In the present study, w.. Read More»

Genet. Mol. Res. 12(3):
2013.July.8.6
DOI:
10.4238/2013.July.8.6
Medical Genetics   Research Article

Association of ectomycorrhizal fungi with Picea crassifolia (Pinaceae, Piceoidae) from high-altitude stands in Mount Helan Nature Reserve, China

Authors: Y.J. Fan, T. Grebenc, J. Wei, Y.L. Zhao, W. Yan and L.B. Wang

We investigated the diversity of ectomycorrhiza associated with the endemic Picea crassifolia in Mount Helan National Nature Reserve in Inner Mongolia, China. Toward this objective, we conducted morphological and molecular identification of ectomycorrhizae in soil cubes taken from pure P. crassifolia stands. Eleven types of ec.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038604
DOI:
10.4238/gmr.15038604
Bioinformatics   Research Article

Construction and analysis of a subtractive cDNA library of early embryonic development in duck

Authors: Y.L. Liu, L.X. Zhong, J.J. Li, J.D. Shen, D.Q. Wang, Z.R. Tao, F.X. Shi and L.Z. Lu

Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early develo.. Read More»

Genet. Mol. Res. 12(3):
2013.July.8.5
DOI:
10.4238/2013.July.8.5
Human Genetics   Research Article

Role of ADH2 and ALDH2 gene polymorphisms in the development of Parkinson�s disease in a Chinese population

Authors: C.C. Zhao, H.B. Cai, H. Wang and S.Y. Pan

In this study, we investigated the role of ADH2 Arg47His and ALDH2 Glu487Lys genetic polymorphisms in the development of Parkinson’s disease in a Chinese population. Between January 2013 and May 2014, 115 patients with Parkinson’s disease and 214 healthy controls were recruited in our study. Genotyping of ADH2 Arg4.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038606
DOI:
10.4238/gmr.15038606
Animal Genetics   Research Article

Effect of organic matter enrichment on the fungal community in limestone cave sediments

Authors: E.L.S. Marques, J.C.T. Dias, G.S. Silva, C.P. Pirovani and R.P. Rezende

Caves are considered major touristic attractions. The management plans of many such caves include limiting the number of visitors; however, the human impact on microbial communities within caves is rarely considered. Therefore, the aim of this study was to evaluate the impact of human-transferred organic matter on the fungal m.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038611
DOI:
10.4238/gmr.15038611
Cytogenomics   Research Article

Isolation and characterization of new polymorphic microsatellite markers from the cuttlefish Sepiella maindroni (Cephalopoda; Sepiidae)

Authors: B.-Y. Guo, P.Z. Qi, A.Y. Zhu, Z.M. Lv, W.C. Wang and C.W. Wu

Fifteen new polymorphic microsatellite loci were developed for the cuttlefish Sepiella maindroni. In 32 individuals from a wild population of coastal Ningde, Fujian Province, China, the number of alleles at these loci varied between 2 and 12, with an average of 5.86. The mean observed and expected heterozygosities were 0.6917 and 0.5993, respectively. Amo.. Read More»

Genet. Mol. Res. 12(3):
2013.July.11.1
DOI:
10.4238/2013.July.11.1
Microbial Genetics   Research Article

Association between VEGF and eNOS gene polymorphisms and lumbar disc degeneration in a young Korean population

Authors: I.B. Han, A.E. Ropper, Y.D. Teng, D.A. Shin, Y.J. Jeon, H.M. Park, D.E. Shin, Y.S. Park, K.N. Kim and N.-K. Kim

Disturbances in blood flow to intervertebral discs (IVD) play an important role in IVD degeneration. Vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) are extremely important angiogenic factors for vasodilation and neovascularization. We investigated the relationship between single nucleotide polymorphisms (SNPs) of th.. Read More»

Genet. Mol. Res. 12(3):
2013.July.8.10
DOI:
10.4238/2013.July.8.10
Cytogenomics   Research Article

Recycling of superfine resolution agarose gel

Authors: T.-Y. Seng, R. Singh, Q.Z. Faridah, S.-G. Tan and S.S.R.S. Alwee

Genetic markers are now routinely used in a wide range of applications, from forensic DNA analysis to marker-assisted plant and animal breeding. The usual practice in such work is to extract the DNA, prime the markers of interest, and sift them out by electrically driving them through an appropriate matrix, usually a gel. The gels, made from polyacrylamid.. Read More»

Genet. Mol. Res. 12(3):
2013.March.11.1
DOI:
10.4238/2013.March.11.1
Microbial Genetics   Research Article

Characterization and description of a multiple sex chromosome system in Potamotrygon motoro (Chondrichthyes, Myliobatiformes) from the Paran�¡ River, Argentina

Authors: D.R. Aichino, M.C. Pastori, H.A. Roncati, M.A. Ledesma, A.C. Swar�§a and A.S. Fenocchio

The Potamotrygonidae family represents the only freshwater group of specialized elasmobranchs and is composed of 4 genera: Plesiotrygon, Paratrygon, Potamotrygon, and Heliotrygon. Individuals of Potamotrygon motoro were collected from the Paraná River at Ituzaingó (Province of Corrientes) in Argentina. Mitotic preparations were obtained usin.. Read More»

Genet. Mol. Res. 12(3):
2013.January.16.3
DOI:
10.4238/2013.January.16.3