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Plant Genetics   Research Article

Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes

Authors: M. Guo, Y.L. Zhang, Z.J. Meng and J. Jiang

Micro-Tom is the smallest known variety of tomatoes. An orthogonal experimental design L16 (45) was used to optimize Agrobacterium-mediated transformation of cotyledon explants of Lycopersicon esculentum cv. Micro-Tom. Four parameters were investigated to determine their effect on transformation frequency: the concentration of.. Read More»

Genet. Mol. Res. 11(1):
2012.March.16.4
DOI:
10.4238/2012.March.16.4
Microbial Genetics   Research Article

Association of TLR2 and TLR4 non-missense single nucleotide polymorphisms with type 2 diabetes risk in a southern Chinese population: a case-control study

Authors: W.H. Huang, L.H. Nie, L.J. Zhang, L.P. Jing, F. Dong, M. Wang, N. Zhang, Y. Liu, B.H. Zhang, C. Chen, H.S. Lin, X.C. Wei, G. Yang and C.X. Jing

Toll-like receptors (TLRs), the triggers of the innate and adaptive immune responses, are involved in the pathogenesis of type 2 diabetes mellitus (T2DM). Several studies have investigated the effects of genetic polymorphisms in TLR4 and TLR2, but they have yielded limited results. We investigated whether non-missense geneticpolymorphisms in the regulator.. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.18
DOI:
10.4238/2015.July.31.18
Animal Genetics   Research Article

Polymorphisms of the ATP1A1 gene associated with mastitis in dairy cattle

Authors: Y.X. Liu, C.H. Xu, T.Y. Gao and Y. Sun

Mastitis affects the concentrations of potassium and sodium in milk. Since sodium-potassium adenosine triphosphatase (Na+, K+-ATPase) is critical for maintaining the homeostasis of these two ions, and is involved in cell apoptosis and pathogenesis, we presumed that polymorphism of the ATP1A1 gene, which encodes the bovine Na+,.. Read More»

Genet. Mol. Res. 11(1):
2012.March.16.3
DOI:
10.4238/2012.March.16.3
Animal Genetics   Research Article

Detection of Toxoplasma gondii DNA in naturally infected sheep�s milk

Authors: D. de Santana Rocha, R.L. de Sousa Moura, B.M. Maciel, L.A. Guimar�£es, H.N.S. Oâ��dwyer, A.D. Munhoz and G.R. Albuquerque

The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indir.. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.14
DOI:
10.4238/2015.July.31.14
Microbial Genetics   Research Article

Related factors and prevalence of Parkinson�s disease among Uygur residents in Hetian, Xinjiang Uygur Autonomous Region

Authors: X.L. Yang, Q. Luo, H.X. Song, Y.L. Wang, Y.N. Yao and H. Xia

The aim of this study was to identify the factors related to and determine the prevalence of ParkinsonâÃ?Â?Ã?Â?s disease (PD) among Uygur residents in Hetian Prefecture, Xinjiang Uygur Autonomous Region. This population-based prospective cohort study used structured questionnaires to screen for factors related to PD. The prevalence in different age .. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.1
DOI:
10.4238/2015.July.31.1
Medical Genetics   Research Article

Adjuvant chemotherapy may improve survival of patients with luminal A breast cancer and positive lymph nodes

Authors: Y. Han, Q. Li, B.H. Xu, P. Zhang, P. Yuan, J.Y. Wang, F. Ma, R.G. Cai and Y. Fan

The study examined the clinicopathological characteristics and treatment options in patients with luminal A breast cancer. This retrospective cohort included 1580 patients with luminal A breast cancer treated between January 2005 and June 2007. Patients were divided into four subgroups according to lymph node status. Prognostic factors and 5-year overall .. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.4
DOI:
10.4238/2015.July.31.4
Plant Genetics   Research Article

Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers

Authors: I. Ullah, A. Iram, M.Z. Iqbal, M. Nawaz, S.M. Hasni and S. Jamil

The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence.. Read More»

Genet. Mol. Res. 11(1):
2012.March.14.3
DOI:
10.4238/2012.March.14.3
Medical Genetics   Research Article

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

Authors: G.E. Oliveira, E.V.R. Von Pinho, T. Andrade, J.C. Souza, F. Caixeta and R.A.D.C. Ferreira

In this study, we analyzed heterosis, amylase enzyme gene expression, and the physiological quality of maize seeds with different genotypes and sizes, which were subjected to aging and not subjected to aging. We used seeds from 2 maize lines that differed with regard to physiological quality, the hybrid, and the reciprocal hybrid; they were classified int.. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.10
DOI:
10.4238/2015.July.31.10
Microbial Genetics   Research Article

Dynamic QTL analysis of protein content and glutamine synthetase activity in recombinant inbred wheat lines

Authors: H.M. Li, H. Liang, Z. Li, Z.X. Tang, S.L. Fu, Y.Y. Geng, B.J. Yan and Z.L. Ren

Protein content (PC) is a crucial factor that determines the end-use and nutritional quality of wheat (Triticum aestivum). Glutamine synthetase (GS), which is a major participant in nitrogen metabolism, can convert inorganic nitrogen into organic nitrogen. Although many studies have been conducted on PC and GS, a dynamic analysis of all of the filling sta.. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.19
DOI:
10.4238/2015.July.31.19
Plant Genetics   Research Article

rDNA ITS sequences among morphotypes of Keratell cochlearis, Keratell quadrata and Brachionus forficula (Rotifera)

Authors: Y.L. Ge, Y.L. Xi, J. Ma and D.D. Xu

Morphological variation in rotifers is affected by environmental conditions, making it hard to identify some rotifer taxa. We examined the rDNA ITS sequences of 10 unspined (KCU1-KCU10) and 17 spined (KCS1-KCS17) Keratell cochlearis clones, 26 two-spined (KQT1-KQT26), 18 single-spined (KQS1-KQS18) and 9 unspined (KQU1-KQU9) K... Read More»

Genet. Mol. Res. 11(1):
2012.March.22.7
DOI:
10.4238/2012.March.22.7