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Proteins differentially expressed by Shiga toxin-producing Escherichia coli strain M03 due to the biliar salt sodium deoxycholate
Authors: C.B.A. Ribeiro1, M.G. Sobral1, C.L. Tanaka1, C.B. Dallagassa1, G. Picheth1, F.G.M. Rego1, D. Alberton1, L.F. Huergo2, F.O. Pedrosa2, E.M. Souza2 and C.M.T. Fadel-Picheth1
Shiga toxin-producing Escherichia coli (STEC) can cause conditions ranging from diarrhea to potentially fatal hemolytic uremic syndrome. Enteropathogen adaptation to the intestinal environment is necessary for the development of infection, and response to bile is an essential characteristic. We evaluated the response of STEC strain M03 to the bile salt so.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.1
- DOI:
- 10.4238/2013.October.24.1
A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.)
Authors: J.W. Wang, H.Q. Wang, W.W. Xiang and T.Y. Chai
We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructe.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.4
Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia
Authors: M.A. Intan Sakinah, I.V. Suzianti and Z. Latiffah
Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Coll.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.5
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.5
Expression analysis of differentially expressed miRNAs in male and female chicken embryos
Authors: Y.P. Feng, J.F. Chen, P. Huang, X. Wang, J. Wang, X.L. Peng and Y.Z. Gong
MicroRNAs (miRNAs) are small non-coding RNA molecules that play key roles in the regulation of development processes of many tissues and organs at the post-transcriptional level. However, little is known about how they affect chicken gonadal development. We examined the expression of four miRNAs (miR-218, -200b, -196, and -206.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.2
- DOI:
- 10.4238/2014.April.17.2
Genetic association of catechol-O-methyltransferase val(158)met polymorphism in Saudi schizophrenia patients
Authors: S. Al-Asmary, S. Kadasah, M. Arfin, M. Tariq and A. Al-Asmari
Schizophrenia is a complex neuropsychiatric disorder strongly associated with dopamine dysregulation. Catechol-O-methyl-transferase (COMT) is a candidate gene for schizophrenia that encodes an enzyme involved in the metabolic inactivation of dopamine. The COMT Val158Met polymorphism has been associated with schizophrenia and h.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.4
- DOI:
- 10.4238/2014.April.17.4
Proteomic response of Moniliophthora perniciosa exposed to pathogenesis-related protein-10 from Theobroma cacao
Authors: F.A.C. Silva1, C.P. Pirovani1, S. Menezes1, C. Pungartnik2, A.S. Santiago1, M.G.C. Costa1, F. Micheli3 and A.S. Gesteira4
TcPR-10, a member of the pathogenesis-related protein 10 family, was identified in EST library of interactions between Theobroma cacao and Moniliophthora perniciosa. TcPR-10 has been shown to have antifungal and ribonuclease activities in vitro. This study aimed to identify proteins that are differentially expressed in M. perniciosa in response to TcPR-10.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.5
- DOI:
- 10.4238/2013.October.22.5
Molecular detection of QTL controlling plant height components in a doubled haploid barley population
Authors: X.F. Ren, D.F. Sun, W.B. Dong, G.L. Sun and C.D. Li
Yield losses caused by lodging in barley can be partially controlled by reducing plant height. In order to understand dwarfing mechanisms and efficiently use new dwarf germplasms for a breeding program, it is important to identify QTL of plant height components. QTL analysis was performed for seven plant height component traits using a DH population of 12.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.5
- DOI:
- 10.4238/2014.April.17.5
Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes
Authors: C.R. Rainho, S.M. Corr�?ªa, J.L. Mazzei, C.A.F. Aiub and I. Felzenszwalb
The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sen.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.9
Rapid method for DNA extraction from the honey bee Apis mellifera and the parasitic bee mite Varroa destructor using lysis buffer and proteinase K
Authors: M.R.C. Issa1, V.L.C. Figueiredo2, D. De Jong1, C.H. Sakamoto1 and Z.L.P. Sim�?µes2
We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, tho.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.4
- DOI:
- 10.4238/2013.October.22.4
Clinical features of diabetes mellitus cases complicated by Burkholderia pseudomallei septicemia
Authors: H.B. Quan, T.Y. Li, Y.Y. Gao and D.X. Chen
The aim of this study was to analyze the clinical characteristics of diabetes mellitus patients with Burkholderia pseudomallei septicemia and evaluate strategies of diagnosis and treatment. The clinical characteristics, diagnosis, treatment, and prognosis of 39 diabetes mellitus patients with B. pseudomallei septicemia were re.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.7
- DOI:
- 10.4238/2014.April.17.7