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Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge
Authors: Q.Z. Yang, Z.J. Yang, Y. Zhang, X.L. Li and W. Zhang
A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.1
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.1
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
Authors: L.N. Sun1,2, H.S. Yang1, M.Y. Chen3 and D.X. Xu1,2
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.7
- DOI:
- 10.4238/2013.November.7.7
Development of 1047 insertion-deletion markers for rice genetic studies and breeding
Authors: Y.X. Zeng*, Z.H. Wen*, L.Y. Ma, Z.J. Ji, X.M. Li and C.D. Yang
In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and j.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.7
- DOI:
- 10.4238/2013.October.30.7
Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)
Authors: W. Zhao1,2, L. Chen1, F. Zhang1, P. Wu1, E. Li1 and J. Qin3
We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino aci.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.6
- DOI:
- 10.4238/2013.October.30.6
Effect of variation of ABCB1 and GSTP1 on osteosarcoma survival after chemotherapy
Authors: J.Z. Li, Z.Q. Tian, S.N. Jiang and T. Feng
We conducted a comprehensive study to investigate the role of genes involved in metabolic and transport pathways in response to chemotherapy and clinical outcome of osteosarcoma patients. Genotyping of seven gene polymorphisms was performed on a 384-well plate format on the Sequenom MassARRAY platform in 162 patients with oste.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.3
- DOI:
- 10.4238/2014.April.25.3
Clinical application of carbon nanoparticle lymph node tracer in the VI region lymph node dissection of differentiated thyroid cancer
Authors: S.P. Sun, Y. Zhang, Z.Q. Cui, Q. Chen, W. Zhang, C.X. Zhou, P.P. Xie and B.G. Liu
The application and clinical significance of carbon nanoparticle lymph tracer in the VI region (central region) lymph node dissection of differentiated thyroid cancer was investigated. Eighty patients with differentiated thyroid cancer were equally divided into the carbon nanoparticle-marked group (ipsilateral thyroid injectio.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.4
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.4
Optimization of a biolistic transformation system for transfer of antifreeze gene KN2 and the bar herbicide resistance gene in common wheat
Authors: L. Cai, D.F. Sun and G.L. Sun
We studied the effects of different media for callus induction and differentiation, and pre-culture period of immature wheat embryo culture on biolistic transformation efficiency for including antifreeze gene KN2 and bar conferring resistance to the herbicide bialaphos. The percentage of plantlets generated from induction and .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.8
Association of the CYP1A1 MspI and TNF�±-308 polymorphisms with chronic obstructive pulmonary disease in Inner Mongolia
Authors: L. Yang, F. Li2*, M. Yan and X. Su
Chronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by persistent airflow limitation. Smoking, occupational exposures, air pollution, and genetics are all risk factors. In the present study, we detected the cytochrome P4501A1 gene (CYP1A1) MspI polymorphism and the tumor necrosis factor alp.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.6
- DOI:
- 10.4238/2014.April.25.6
Ginsenoside Rh2 promotes nonamyloidgenic cleavage of amyloid precursor protein via a cholesterol-dependent pathway
Authors: J. Qiu, W. Li, S.H. Feng, M. Wang and Z.Y. He
Ginsenoside Rh2 (Rh2) is a ginseng derivative used in Chinese traditional medicine. We investigated whether Rh2 can help prevent Alzheimer’s disease symptoms and examined underlying mechanisms. We injected Rh2 into tg2576 Alzheimer’s disease model mice and looked for behavioral improvement and senile plaque reducti.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.2
Evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic DNA markers associated with the resistant trait in Chinese wild Vitis
Authors: J. Zhang, Y. Zhang, H. Yu1, and Y. Wang
The resistance of wild Vitis germplasm, including Chinese and American wild Vitis and Vitis vinifera cultivars, to powdery mildew (Uncinula necator Burr.) was evaluated for two consecutive years under natural conditions. Most of the Chinese and North American species displayed a resistant phenotype, whereas all of the European.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.3
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.3