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Analysis of DNA polymerase activity in vitro using non-radioactive primer extension assay in an automated DNA sequencer
Authors: Lopes DO, Regis-da-Silva CG, Machado-Silva A, Macedo AM, Franco GR, Hoffmann JS, Cazaux C, Pena SDJ, Teixeira SMR and Machado CR
Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used routinely for many years for DNA sequ.. Read More»
- Genet. Mol. Res. 6(2):
Endophytic bacteria from Piper tuberculatum Jacq.: isolation, molecular characterization, and in vitro screening for the control of Fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (Piper nigrum L.)
Authors: S.B. Nascimento, A.M. Lima, B.N. Borges and C.R.B. de Souza
Endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. In this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae Piper tuberculatum, which is known for itsresistance to infection by Fusariu.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.32
- DOI:
- 10.4238/2015.July.3.32
Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene
Authors: Q.Q. Song, Z.X. Chai, J.W. Xin, S.J. Zhao, Q.M. Ji, C.F. Zhang, Z.J. Ma and J.C. Zhong
To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.13.3
- DOI:
- 10.4238/2015.March.13.3
Development of expressed sequence tag-simple sequence repeat markers for Chrysanthemum morifolium and closely related species
Authors: H. Liu, Q.X. Zhang, M. Sun, H.T. Pan and Z.X. Kong
With the development of chrysanthemum breeding in recent years, an increasing number of wild species in genera related to Chrysanthemum were introduced to extend the genetic resources and facilitate the genetic improvement of chrysanthemums via hybridization. However, few simple sequence repeat (SSR) markers are available for marker-assisted breeding and .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.1
- DOI:
- 10.4238/2015.July.13.1
Selection and estimation of the heritability of sunflower (Helianthus annuus) pollen collection behavior in Apis mellifera colonies
Authors: Basualdo M, RodrÃ?Âguez EM, Bedascarrasbure E and De Jong D
We selected honey bee colonies (Apis mellifera L.) with a high tendency to collect sunflower pollen and estimated the heritability of this trait. The percentage of sunflower pollen collected by 74 colonies was evaluated. Five colonies that collected the highest percentages of sunflower pollen were selected. Nineteen colonies h.. Read More»
- Genet. Mol. Res. 6(2):
Lack of association between MD-2 promoter gene variants and tuberculosis
Authors: Y. Xue, Z.Q. Zhao, D. Hong, M.Y. Zhao, Y.X. Zhang, H.J. Wang, Y. Wang and J.C. Li
Myeloid differentiation-2 (MD-2) is an essential component of the CD14-TLR4/MD-2 receptor complex involved in microbial cell wall component recognition during infection. Genetic variations in the MD-2 gene may influence human susceptibility to infectious diseases. To date, a predisposition of MD-2 gene variants to contract tub.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr771
- DOI:
- 10.4238/vol9-3gmr771
Genetic diversity and population structure of Kazakh horses (Equus caballus) inferred from mtDNA sequences
Authors: M. Gemingguli, K.R. Iskhan, Y. Li, A. Qi, W. Wunirifu, L.Y. Ding and A. Wumaierjiang
The Kazakh horse is an important old horse breed in Xinjiang. They have contributed greatly to the breeding and improvement of other local horse breeds, yet their genetic diversity and population structure are not well understood. In the present study, we evaluated the genetic diversity of Kazakh horses and their relationship .. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048618
- DOI:
- 10.4238/gmr.15048618
Synergistic effect of BMP9 and TGF-�² in the proliferation and differentiation of osteoblasts
Authors: X.L. Li, Y.B. Liu, E.G. Ma, W.X. Shen, H. Li and Y.N. Zhang
We investigated the synergistic effect of bone morphogenetic protein 9 (BMP9) and transforming growth factor (TGF)-b in the transformation of mesenchymal stem cells into osteoblasts. We evaluated the effect of BMP9 and TGF-b on the induction of osteoblast formation. Mitogen-activated protein kinase (MAPK) pathway-related proteins such as p38, extracellula.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.4
- DOI:
- 10.4238/2015.July.13.4
Radiofrequency thermocoagulation combined with pulsed radiofrequency helps relieve postoperative complications of trigeminal neuralgia
Authors: W.-X. Zhao, Q. Wang, M.-W. He, L.-Q. Yang, B.-S. Wu and J.-X. Ni
Trigeminal neuralgia is a sudden, severe condition characterized by stabbing and recurrent pain. Radiofrequency thermocoagulation (RFT) and pulsed radiofrequency (PRF) are common surgical interventions used to treat trigeminal neuralgia. This study aimed to investigate the therapeutic effects and associated complications of a combination of RFT and PRF in.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.5
- DOI:
- 10.4238/2015.July.13.5
Role of APN and TNF-�± in type 2 diabetes mellitus complicated by nonalcoholic fatty liver disease
Authors: X. Lin, Z. Zhang, J.M. Chen, Y.Y. Xu, H.R. Ye, J. Cui, Y. Fang,Y. Jin, D.R. Zhu and L. Yuan
Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by non-excessive alcohol consumption and is the most common cause of elevated levels of serum liver enzymes. We examined changes in adiponectin (APN) and tumor necrosis factor-a (TNF-a) in type 2 diabetes mellitus (T2DM) complicated by NAFLD and their relationships with insulin resis.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.1
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.1