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Plant Genetics   Research Article

Characterization of Genetic Diversity and Linkage Disequilibrium of ZmNAC33 in Maize

Authors: Wenping Liu, Chunxiao Zhang, Shufang Li, Fengxue Jin, Wanjun Li, Xiaohui Li

The maize NAC gene, encoding a NAC domain transcription factor, plays vital roles in the growth process of maize under abiotic stresses. To investigate the response of ZmNAC33 to salinity stress, we analyzed the expression of the ZmNAC33 gene in roots and leaves of three-leaf stage seedlings among maize inbred lines. The resul.. Read More»

Genet. Mol. Res. 17(1):
gmr16039879
DOI:
10.4238/gmr16039879
Plant Genetics   Research Article

Genome-wide analysis of salinity-stress induced DNA methylation alterations in cotton (Gossypium hirsutum L.) using the Me-DIP sequencing technology

Authors: X.K. Lu, N. Shu, J.J. Wang, X.G. Chen, D.L. Wang, S. Wang, W.L. Fan, X.N. Guo, L.X. Guo and W.W. Ye

Cytosine DNA methylation is a significant form of DNA modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. Although the reference genomes of cotton (Gossypium hirsutum L.) have been publically available, the salinity-stress-induced DNA methylome alterations in cotton are not well unde.. Read More»

Genet. Mol. Res. 16(2):
gmr16029673
DOI:
10.4238/gmr16029673
Animal Genetics   Research Article

Effects of various salinities on Na+-K+-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis

Authors: M. Sun, K. Jiang, F. Zhang, D. Zhang, A. Shen, M. Jiang, X. Shen and L. Ma

Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0, 2, 5, 10, a.. Read More»

Genet. Mol. Res. 11(2):
2012.April.19.3
DOI:
10.4238/2012.April.19.3
Plant Genetics   Research Article

Digital expression analysis of the genes associated with salinity resistance after overexpression of a stress-responsive small GTP-binding RabG protein in peanut

Authors: J.M. Sui, G. Li, G.X. Chen, M.Y. Yu, S.T. Ding, J.S. Wang and L.X. Qiao

The Rab protein family is the largest family of the small GTP-binding proteins. Among them, the RabG genes are known to be responsive to abiotic stresses, but the molecular mechanisms of the stress responses mediated by RabG genes in plants is poorly understood. To investigate the molecular mechanism of AhRabG gene in peanut, .. Read More»

Genet. Mol. Res. 16(1):
gmr16019432
DOI:
10.4238/gmr16019432
Human Genetics   Research Article

Gene expressions levels of 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in gill tissue of Mugil cephalus acclimated to low salinity

Authors: L. Li, M. Jiang and X.Q. Shen

Fishes adapt to salinity changes primarily through osmotic pressure regulation, a process often associated with several genes, including 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ. The present study investigated the differential expression of genes 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in the gill tissue of Mu.. Read More»

Genet. Mol. Res. 16(1):
gmr16019444
DOI:
10.4238/gmr16019444
Plant Genetics   Research Article

Salt stress alters DNA methylation levels in alfalfa (Medicago spp)

Authors: A. Al-Lawati1, S. Al-Bahry1, R. Victor1, A.H. Al-Lawati2 and M.W. Yaish1

Modification of DNA methylation status is one of the mechanisms used by plants to adjust gene expression at both the transcriptional and posttranscriptional levels when plants are exposed to suboptimal conditions. Under abiotic stress, different cultivars often show heritable phenotypic variation accompanied by epigenetic polymorphisms at the DNA methylat.. Read More»

Genet. Mol. Res. 15(1):
0.4238/gmr.15018299
DOI:
0.4238/gmr.15018299
Plant Genetics   Research Article

Computational prediction and experimental validation of a novel miRNA in Suaeda maritima, a halophyte

Authors: S.A. Gharat and B.P. Shaw

The lack of available transcriptome data for plants of no economic or agronomic importance limits the identification of miRNAs in many species. Considering the possible similarity of the transcriptome between related species, the present study used expressed sequence tags (ESTs) of Suaeda salsa and Suaeda glauca to identify conserved miRNAs, which were va.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017527
DOI:
10.4238/gmr.15017527
Microbial Genetics   Research Article

Molecular cloning, characterization, and expression of vacuolar-type-H+-ATPase B1 (VHAB1) gene in the gill of Anguilla marmorata

Authors: L. Li, Y.H. Jia, P. Li, S.W. Yin, G.S. Zhang, X.L. Wang, Y.Y. Wang and X.J. Wang

We explored the molecular mechanism of the regulation of vacuolar-type-H+-ATPase B1 (VHAB1) in elvers in the response to salinity. The full-length cDNA of VHAB1 in Anguilla marmorata (designated as AmVHAB1), which was 1741 base pairs (bp) in length, was found to encompass a 1512-bp open reading frame encoding a polypeptide with 503 amino acids (55.9 kDa),.. Read More»

Genet. Mol. Res. 14(3):
2015.July.17.9
DOI:
10.4238/2015.July.17.9
Plant Genetics   Research Article

Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress

Authors: G.P. Moraes, L.C. Benitez, M.N. do Amaral, I.L. Vighi, P.A. Auler, L.C. da Maia, V.J. Bianchi and E.J.B. Braga

To obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (RT-qPCR) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions. In this study, the stability of expression was evalu.. Read More»

Genet. Mol. Res. 14(1):
2015.March.27.24
DOI:
10.4238/2015.March.27.24
Plant Genetics   Research Article

Changes in gene expression and catalase activity in Oryza sativa L. under abiotic stress

Authors: I.L. Vighi, L.C. Benitez, M.N. do Amaral, P.A. Auler, G.P. Moraes, G.S. Rodrigues, L.C. da Maia, L.S. Pinto and E.J.B. Braga

Different rice (Oryza sativa L.) genotypes were subjected to high salinity and low temperature (150 mM NaCl and 13°C, respectively) for 0, 6, 24, 48, or 72 h. We evaluated the simultaneous expression of the genes OsCATA, OsCATB, and OsCATC, correlated gene expression with enzyme activity, and verified the regulation of the.. Read More»

Genet. Mol. Res. 15(4):
gmr15048977
DOI:
10.4238/gmr15048977