Microsatellites
Molecular characterization of the Andean blackberry, Rubus glaucus, using SSR markers
Authors: M. Marulanda, A.M. L�³pez and M. Uribe
The species Rubus glaucus, also known as the Andean or “Castilla” blackberry, is one of nine edible species of this genus that grow naturally in Central and South America. In Colombia, this species is the most important of all Rubus species for agricultural and commercial purposes. We used 20 SSRs developed for oth.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.10.3
- DOI:
- 10.4238/2012.February.10.3
Isolation and characterization of novel microsatellite markers in commercial selected golden Malaysian arowana fish, Scleropages formosus (Osteoglossidae)
Authors: B. Manoharan, Z. Sulaimen, F. Omar, R.Y. Othman, S.Z. Mohamed and S. Bhassu
Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr944
- DOI:
- 10.4238/vol10-2gmr944
Polymorphisms of the cocaine-amphetamine-regulated transcript (CART) gene and their association with reproductive traits in Chinese goats
Authors: P.Q. Wang, L.M. Deng, B.Y. Zhang, M.X. Chu and J.Z. Hou
Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorp.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1091
- DOI:
- 10.4238/vol10-2gmr1091
Isolation and characterization of 21 novel polymorphic microsatellite loci in the Chinese soft-shelled turtle Pelodiscus sinensis
Authors: X.J. Bu, L. Liu, L. Wang and L.W. Nie
Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and det.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1119
- DOI:
- 10.4238/vol10-2gmr1119
Segregation and genetic linkage analyses of river catfish, Mystus nemurus, based on microsatellite markers
Authors: B.P. Hoh, S.S. Siraj, S.G. Tan and K. Yusoff
The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedi.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.February.28.1
- DOI:
- 10.4238/2013.February.28.1
Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda
Authors: S.W. Jia, P. Liu, J. Li, J.T. Li and L.Q. Pan
The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.8.1
- DOI:
- 10.4238/2013.August.8.1
Population structure and genetic diversity of maize inbreds derived from tropical hybrids
Authors: E.C.M. Lanes, J.M.S. Viana, G.P. Paes, M.F.B. Paula, C. Maia, E.T. Caixeta and G.V. Miranda
The objectives of this study were to identify the population structure and to assess the genetic diversity of maize inbreds. We genotyped 81 microsatellite loci of 90 maize inbreds that were derived from tropical hybrids and populations. The population structure analysis was based on a Bayesian approach. Each subpopulation was.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.2
- DOI:
- 10.4238/2014.September.12.2
Isolation and characterization of microsatellite markers in Atrina vexillum Born
Authors: Y.F. Ning, Z.B. Li, Y.S. Huang, X.Q. Mao, B.B. Li, Y. Yuan and J.B. Shangguan
Atrina vexillum Born is an economically valuable species, widely distributed in the coastal waters of temperate and tropical areas of the Asia Pacific region. Twenty one novel microsatellite loci were identified in the genome of A. vexillum Born using the protocol for fast isolation by amplified fragment length polymorphism of.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027204
- DOI:
- 10.4238/gmr.15027204
Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines
Authors: A.A. Leal, C.A. Mangolin, A.T. do Amaral J�ºnior, L.S.A. Gon�§alves, C.A. Scapim, A.S. Mott, I.B.O. Eloi, V. Cordov�©s and M.F.P. da Silva
Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr692
- DOI:
- 10.4238/vol9-1gmr692
Microsatellite usefulness is independent of phylogenetic distance in Tyrant flycatchers (Aves: Tyrannidae): a test using two globally threatened species
Authors: B. Mahler, A.R.R. Schneider, A.S. Di Giacomo, A.G. Di Giacomo, J.C. Reboreda and R. Tiedemann
Tyrant flycatchers (Aves: Tyrannidae) are endemic to the New World, and many species of this group are threatened or near-threatened at the global level. The aim of this study was to test the 18 microsatellite markers that have been published for other Tyrant flycatchers in the Strange-tailed Tyrant (Alectrurus risora) and the Sharp-tailed Tyrant (Culiciv.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.12
- DOI:
- 10.4238/2013.August.12.12