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Microsatellites

Animal Genetics   Research Article

Study of closely related species within the Physalaemus cuvieri group (Anura): contribution of microsatellite markers

Authors: M. Conte, M.I. Zucchi, G.V. Andrade, A.P. Souza and S.M. Recco-Pimentel

Various species of the Physalaemus cuvieri group of frogs are difficult to distinguish morphologically, making molecular analysis an attractive alternative for indentifying members of this group, which is considered to be at risk because of loss of habitat. The genetic structure of natural populations of P. ephippifer and P. a.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1136
DOI:
10.4238/vol10-3gmr1136
Animal Genetics   Research Article

Allele frequencies and genetic diversity in two groups of wild tufted capuchin monkeys (Cebus apella nigritus) living in an urban forest fragment

Authors: Jeanne Margareth Jimenes Amaral, Aguinaldo Luiz Sim�µes and David De Jong

There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part,.. Read More»

Genet. Mol. Res. 4(4):
Microbial Genetics   Research Article

Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae)

Authors: R.O. Manoel, M.L.M. Freitas, E.V. Tambarussi, J. Cambuim, M.L.T. Moraes and A.M. Sebbenn

Genipa americana is a tropical tree species that is widely distributed in the humid tropical and subtropical regions of Central and South America. This study investigated Mendelian inheritance, genetic linkage, and genotypic disequilibrium at six microsatellite loci developed for G. americana. Adult trees (188) and regenerants (163) were sampled and genot.. Read More»

Genet. Mol. Res. 14(3):
2015.July.27.4
DOI:
10.4238/2015.July.27.4
Medical Genetics   Research Article

Development of polymorphic microsatellite loci in Odontobutis obscura using Illumina paired-end sequencing and the test of cross-species amplification

Authors: H.-G. Bae, J. An, J. Jun, S.-M. Kweon, S. Kim, J.-N. Yu, B.-J. Kim, H. Sakai, Y.J. Lee and H.Y. Suk

Odontobutis obscura is a bottom-dwelling freshwater fish native to East Asia. Its range encompasses southwest China, western Japan, and Geoje Island in South Korea. Despite its widespread range in China and Japan, only a small and spatially isolated population is found in South Korea. We developed a total of 23 novel and polym.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038405
DOI:
10.4238/gmr.15038405
Cytogenomics   Research Article

Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites

Authors: I.G. Hamoy, E.J.M. Santos and S.E.B. Santos

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtain.. Read More»

Genet. Mol. Res. 7(1):
vol7-1gmr394
DOI:
10.4238/vol7-1gmr394
Plant Genetics   Research Article

Isolation, characterization, and cross-transferability of microsatellite markers from the whitebacked planthopper (Sogatella furcifera)

Authors: L. Zhang, G. Li, G.C. He, L.L. Zhu, R. Qin and S.L. Jing

The whitebacked planthopper Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is one of the most harmful pests of rice. In this study, 18 polymorphic microsatellite markers were developed from S. furcifera genomic libraries using the fast isolation by amplified fragment length polymorphism of sequences containing r.. Read More»

Genet. Mol. Res. 13(3):
2014.August.15.7
DOI:
10.4238/2014.August.15.7
Animal Genetics   Short commentary

Multiplex PCR panel of microsatellite markers for the tambaqui, Colossoma macropomum, developed as a tool for use in conservation and broodstock management

Authors: I.G. Hamoy and S. Santos

The tambaqui, Colossoma macropomum, native to Brazil, is widely used in aquaculture systems. We developed a multiplex PCR panel for this species, comprising 12 microsatellite loci. This panel was used to genotype 73 specimens collected from Juruti, a city in the Brazilian Amazon. The mean number of alleles per locus was 8.8, t.. Read More»

Genet. Mol. Res. 11(1):
http://dx.doi.org/2012.January.26.1
DOI:
http://dx.doi.org/10.4238/2012.January.26.1
Plant Genetics   Research Article

Genetic relationship of cowpea (Vigna unguiculata) varieties from Senegal based on SSR markers

Authors: F.A. Badiane, B.S. Gowda, N. Ciss�©, D. Diouf, O. Sadio and M.P. Timko

Genetic diversity and phylogenetic relationships among 22 local cowpea (Vigna unguiculata) varieties and inbred lines collected throughout Senegal were evaluated using simple sequence repeat molecular markers. A set of 49 primer combinations were developed from cowpea genomic/expressed sequence tags and evaluated for their abi.. Read More»

Genet. Mol. Res. 11(1):
http://dx.doi.org/2012.February.8.4
DOI:
http://dx.doi.org/10.4238/2012.February.8.4
Animal Genetics   Research Article

Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers

Authors: E.H. Giacomoni, G.P. Fern�¡ndez-Stolz and T.R.O. Freitas

The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mea.. Read More»

Genet. Mol. Res. 7(1):
Animal Genetics   Research Article

Estimation of taurindicine hybridization of American Zebu cattle in Brazil

Authors: P. Ripamonte, M. Baccaglini, A.S.M. Cesar, L.G.G. Figueiredo, J.C.C. Balieiro, A.R. Caetano and F.V. Meirelles

Our objective was to estimate Bos primigenius taurus introgression in American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance component com.. Read More»

Genet. Mol. Res. 11(1):
2012.February.17.1
DOI:
10.4238/2012.February.17.1