Microsatellites
New microsatellite markers for bananas (Musa spp)
Authors: E.P. Amorim, P.H. Silva, C.F. Ferreira, V.B.O. Amorim, V.J. Santos, A.D. Vilarinhos, C.M.R. Santos, M.T. Souza J�ºnior and R.N.G. Miller
Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.8
- DOI:
- 10.4238/2012.April.27.8
First isolation and characterization of genomic SSR markers for the giant red shrimp Aristaeomorpha foliacea (Risso, 1827)
Authors: R. Cannas S. Marcias F. Sacco A. Cau A.M. Deiana
Fourteen microsatellite markers were isolated from the giant red shrimp Aristaeomorpha foliacea (Risso, 1827) using the FIASCO protocol (fast isolation by AFLP of sequences containing repeats). Polymorphism was assessed in 30 individuals from two localities of the western Mediterranean basin (N = 20 from Sardinia and N = 10 from Sicily); nine loci showed .. Read More»
- Genet. Mol. Res. 3(4):
- 2012.August.17.1
- DOI:
- 10.4238/2012.August.17.1
Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil
Authors: R. Strapazzon, F.E. Carneiro, J.C.V. Guerra Jr. and G. Moretto
The mite Varroa destructor is an ectoparasite that is considered a major pest for beekeeping with European honey bees. However, Africanized bee colonies are less threatened by this ectoparasite, because infestation levels remain low in these bees. The low reproductive ability of female mites of the Japanese biotype (J), introduced to Brazil early in .. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr567
- DOI:
- 10.4238/vol8-3gmr567
Selecting soybean resistant to the cyst nematode Heterodera glycines using simple sequence repeat (microssatellite) markers
Authors: S.M.C.G. Espindola O.T. Hamawaki A.P. Oliveira C.D.L. Hamawaki R.L. Hamawaki L.M. Takahashi
The soybean cyst nematode (SCN) is a major cause of soybean yield reduction. The objective of this study was to evaluate the efficiency of marker-assisted selection to identify genotypes resistant to SCN race 3 infection, using Sat_168 and Sat-141 resistance quantitative trait loci. The experiment was carried out under greenhouse conditions, using soybean.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016850
- DOI:
- 10.4238/gmr.15016850
Cross-species amplification of selected zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations
Authors: D. Kaczmarczyk
Fifteen sets of PCR primers designed for the amplification of microsatellite loci from Danio rerio and Phoxinus neogaeus Campostoma DNA that have been proven applicable for molecular studies of several species of cyprinids were tested for amplification of microsatellites from lake minnow DNA. The samples were taken from 298 fish inhabiting 6 distinct popu.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.7
- DOI:
- 10.4238/2013.January.24.7
Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing
Authors: L.A. Fusinatto S. Lopes A. Silva-Ferreira J. Alexandrino C.F.B. Haddad C.F.D. Rocha F. Sequeira
The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.15
- DOI:
- 10.4238/2013.January.24.15
Chloroplast diversity of Casearia grandiflora in the Cerrado of Piau� State
Authors: M.F. Costa, A.A. Pereira, J.B. Pinheiro, M.I. Zucchi, A.S.F. Ara�ºjo, R.L.F. Gomes, S.E.S. Valente, M.E.A. Oliveira and A.C.A. Lopes
Casearia grandiflora (Salicaceae) is a typical Cerrado species adapted to disturbed environments, making it useful for restoration projects. Knowledge of genetic diversity is important for establishing conservation strategies for this species. This study aimed to compare chloroplast haplotype diversity and structure of C. gran.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019572
- DOI:
- 10.4238/gmr16019572
Characterization of microsatellite markers in cassava based on microsatellite-AFLP technique
Authors: S. Whankaew, S. Sraphet, R. Thaikert, D.R. Smith and K. Triwitayakorn
We developed molecular markers for cassava based on the microsatellite-amplified fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were developed and used for the analysis of 48 samples of Manihot species, consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthagine.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.2
- DOI:
- 10.4238/2012.May.15.2
Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda)
Authors: Q.H. Gu, B.X. Xiong, Y.T. Zhu, Q. Wang and P.S. Shi
Although it is a major freshwater gastropod species, genetic diversity of Bellamya aeruginosa was completely unknown. Eighteen microsatellite loci were isolated and characterized from (AC)15-enriched genomic libraries of the freshwater snail B. aeruginosa. Most of the 18 loci were successfully amplified and high polymorphic information content values were.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.18.4
- DOI:
- 10.4238/2012.May.18.4
Characterization of the resistance gradient of cacao genotypes (F2 Sca6 x ICS1) to black pod disease using phytopathometric, genetic and molecular approaches
Authors: E.S.L. Santos
-.. Read More»
- Genet. Mol. Res. 8(4):
- vol8-4ta023
- DOI:
- 10.4238/vol8-4ta023