Authors: G.S.C. Buso, A.M.M. Reis, Z.P.S. Amaral and M.E. Ferreira
This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 1.. Read More»
Authors: E.G. Cothran, J.L. Canelon, C. Luis, E. Conant and R. Juras
Various horse populations in the Americas have an origin in Spain; they are remnants of the first livestock introduced to the continent early in the colonial period (16th and 17th centuries). We evaluated genetic variability within the Venezuelan Criollo horse and its relationship with other horse breeds. We observed high leve.. Read More»
Authors: P.P. Wang, M. Ren, S.Q. Chen, S.W. Yin, C. Zhao, H.Y. Zhang, X.R. Li, Q.Q. Cao and G.Q. Zhou
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequen.. Read More»
Authors: M.R. Bakhtiarizadeh, B. Arefnejad, E. Ebrahimie and M. Ebrahimi
Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity.. Read More»
Authors: D.Y. Kim1*, D. Kim2*, S.K. Park2, H. Lee1, H.Y. Suk2 and M.S. Min1
The wrinkled frog, Rana rugosa, is a species complex that inhabits plains and mountains near freshwater bodies throughout East Asia, encompassing China, Korea, Japan, and the Russian Primorye region. Although extensive efforts are required to estimate cryptic diversity in the R. rugosa complex, no specifically designed microsatellite loci are available. H.. Read More»
Authors: Q.H. Wang, Y.S. Guo, Z.D. Wang, X.D. Du and Y.W. Deng
Sipunculus nudus, the peanut worm, is the best known species in its genus. This unsegmented subtidal marine worm is consumed in some parts of Asia and is also used as fish bait. We found 20 microsatellite DNA markers for S. nudus. The number of alleles per polymorphic locus ranged from two to seven in a sample of 39 individual.. Read More»
Authors: J.B. Leal, R.P. Santos and F.A. Gaiotto
The fragments of the Atlantic Forest of southern Bahia have a long history of intense logging and selective cutting. Some tree species, such as jequitibá rosa (Cariniana legalis), have experienced a reduction in their populations with respect to both area and density. To evaluate the possible effects of selective loggin.. Read More»
Authors: J.A.F. Diniz-Filho, R.G. Collevatti, L.J. Chaves, T.N. Soares, J.C. Nabout, T.F. Rangel, D.B. Melo, J.S. Lima and M.P.C. Telles
Many species are expected to suffer strong shifts in their geographic ranges due to climate changes in the next 50 years, with severe consequences for biodiversity patterns and population structure. We used here an ensemble forecast approach for obtaining species’ range in which multiple species distribution models and c.. Read More»
Authors: Yazbeck GM and Kalapothakis E
We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitiv.. Read More»
Authors: X.Y. Du, W. Li, X.Y. Sa, C.L. Li, J. Lu, Y.Z. Wang and Z.W. Chen
Although gerbils have been widely used in many areas of biological research over many years, there is currently no effective genetic quality control system available. In the present study, we sought to establish a microsatellite marker system for quality control and conducted an optimized analysis of 137 microsatellite loci in two laboratory gerbil popula.. Read More»