Animal Genetics   Research Article

Comparing KISS1 and GPR54 genes between the two breeds of goats with low and high prolificacy

Authors: Jie Zheng, Sayed Haidar Abbas Raza, Xiang-Dong Zi, Jian-Yuan Lu

There is increasing evidence that kisspeptin, the peptide product of KISS1 gene and its receptor (GPR54), regulate reproductive processes through actions on other organs than hypothalamus. Therefore, the study for the first time, was conducted to investigate the nucleotide sequences and mRNA expression levels of KISS1 and GPR54 genes in pituitary, endomet.. Read More»

Genet. Mol. Res. 17(4):
http://dx.doi.org/gmr16039922
DOI:
http://dx.doi.org/10.4238/gmr16039922
Animal Genetics   Research Article

Construction and functional study of pGN, a mammary gland-specific expression plasmid

Authors: J. Lin, Q.H. Yu, Q. Zhang and Q. Yang

The aim of this study was to construct a mammary gland-specific expression vector, pGN, and to validate its function in expressing growth hormone (GH) both in vitro and in vivo. First, the GH gene was amplified and inserted downstream of the b-casein 5'-arm. Next, the neo gene was cloned downstream of the b-casein 3'-arm as a .. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.30.1
DOI:
http://dx.doi.org/10.4238/2014.May.30.1
Animal Genetics   Research Article

Molecular cloning and characterization of KISS1 promoter and effect of KISS1 gene mutations on litter size in the goat

Authors: X.P. An, P. Han, J.X. Hou, H.B. Zhao, Y. Yan, T. Ma, F. Fang, F.X. Meng, Y.X. Song, J.G. Wang and B.Y. Cao

Kisspeptins, the product of the KISS1 gene, play an essential role in the regulation of reproductive functions, acting primarily at the hypothalamic level of the gonadotropic axis. We detected polymorphisms of the goat KISS1 gene in 723 individuals from three goat breeds (Xinong Saanen, Guanzhong, and Boer) by DNA pooling, PCR-RFLP, and DNA sequencing met.. Read More»

Genet. Mol. Res. 12(4):
2013.February.28.13
DOI:
10.4238/2013.February.28.13
Animal Genetics   Research Article

Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of Liaoning cashmere goats

Authors: Q.L. Zhang, J.P Li, Y.M. Li, Q. Chang, Y. Chen, H.Z. Jiang, Z.H. Zhao and D. Guo

Vascular endothelial growth factors (VEGFs) play important roles in neovascularization, tissue development, and angiogenesis. In this study, changes in VEGF expression patterns and microvessel density (MVD), and their correlations, were investigated during hair follicle development in epidermal appendages of Liaoning cashmere goats. Polyclonal antibodies .. Read More»

Genet. Mol. Res. 12(4):
2013.December.10.3
DOI:
10.4238/2013.December.10.3
Animal Genetics   Research Article

Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

Authors: E.M.S. Dorneles, J.A. Santana, G.I. Andrade, E.L.S. Santos, A.S. GuimarĂ£es, R.A. Mota, A.S. Santos, A. Miyoshi, V. Azevedo, A.M.G. Gouveia, A.P. Lage and M.B. Heinemann

Corynebacterium pseudotuberculosis, the infectious agent of caseous lymphadenitis (CLA), is responsible for substantial economic losses in goat and sheep production. Molecular characterization of C. pseudotuberculosis isolates by enterobacterial repetitive intergenic consensus (ERIC)-PCR has shown promising results in genotyping strains isolated from shee.. Read More»

Genet. Mol. Res. 11(3):
2012.August.6.9
DOI:
10.4238/2012.August.6.9
Animal Genetics   Research Article

Molecular cloning, polymorphisms, and expression analysis of the RERG gene in indigenous Chinese goats

Authors: M.X. Sui, H.H. Wang and Z.W. Wang

The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations .. Read More»

Genet. Mol. Res. 14(4):
2015.November.24.1
DOI:
10.4238/2015.November.24.1
Animal Genetics   Research Article

Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats

Authors: S.Y. Zhan, L. Chen, L. Li, L.J. Wang, T. Zhong and H.P. Zhang

Insulin-like growth factor-binding proteins (IGFBPs) play a key role in modulating insulin-like growth factors (IGFs), and are considered candidate genes for growth traits in livestock. In this study, we identified the complete coding sequences of IGFBP-1 to -6 in the Nanjiang brown goat, and assessed gene tissue expression pa.. Read More»

Genet. Mol. Res. 14(4):
2015.October.16.22
DOI:
10.4238/2015.October.16.22
Animal Genetics   Research Article

Variability with altitude of major histocompatibility complex-related microsatellite loci in goats from Southwest China

Authors: G.X. E, Y.F. Huang, Y.J. Zhao and R.S. Na

We aimed to use microsatellite BM1258 loci of the major histocompatibility complex (MHC) as an indicator of the influence of genetic diversity of immunity in goats (Dazu Black, Hechuan White, Meigu, and Tibetan goat). In total, 132 animals comprising 50 Dazu Black goats, 24 Hechuan White goats, 34 Meigu goats, and 24 Tibetan goats were examined. Collectiv.. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.26
DOI:
10.4238/2015.November.18.26
Animal Genetics   Research Article

Changes in cFLIP expression in the corpus luteum throughout the estrous cycle of Shibagoats

Authors: H.Z. Jin, Y. Wang, Y. Liu, X.P. Li and Y. Jin

Changes in the expression of the anti-apoptotic protein cFLIP (cellular FLICE inhibitory protein) were examined in the caprine corpus luteum (CL), during development and subsequent maintenance. Corpora lutea at four different stages were collected from Shiba goats, to measure the expression of cFLIP mRNA, protein and immunolocalization. Expression of shor.. Read More»

Genet. Mol. Res. 14(4):
2015.October.21.16
DOI:
10.4238/2015.October.21.16
Animal Genetics   Research Article

Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats

Authors: X.Y. Yin, G.H. Cheng, H.Y. Guo, Q. Wang, Y.J. Li and H. Zhang

Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for lives.. Read More»

Genet. Mol. Res. 16(1):
gmr16019564
DOI:
10.4238/gmr16019564