Gene
Y-STR genetic screening by high-resolution melting analysis
Authors: J.Q. Deng1*, B.Q. Liu2*, Y. Wang3, W. Liu4, J.F. Cai5, R. Long1 and W.H. Li1
Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are requi.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.1501726605/02/2016
- DOI:
- 10.4238/gmr.1501726605/02/2016
Specific BRCA1 gene variations amongst young Moroccan breast cancer patients
Authors: A. Tazzite, S. Nadiffi, D. Kottwitz, M. El Amrani, H. Jouhadi,A. Benider, A. Moumen4 and H. Sefrioui
Germline mutations in the BRCA1 gene are known predictive markers for the development of hereditary breast cancer. Nevertheless, no comprehensive study has been performed targeting the presence and relevance of BRCA1 mutations in Moroccan breast cancer patients. We here present an analysis of BRCA1 gene regions (exon 2 and exo.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.January.31.5
- DOI:
- http://dx.doi.org/10.4238/2014.January.31.5
Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita
Authors: S.P. Ling, S.S. Su, H.M. Zhang, X.S. Zhang, X.Y. Liu, G.F. Pan and Y. Yuan
Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this.. Read More»
- Genet. Mol. Res. 13(4):
- 2014
- DOI:
- 10.4238/2014
Development of cassava periclinal chimera may boost production
Authors: N. Bomfim and N.M.A. Nassar
Plant periclinal chimeras are genotypicmosaics arranged concentrically. Trials to produce them to combine different species have been done, but pratical results have not been achieved. We report for the second time the development of a very productive interspecific periclinal chimera in cassava. It has very large edible roots .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.10.1
- DOI:
- 10.4238/2014.February.10.1
A novel missense mutation in exon 7 of the ECM1 gene in an Iranian lipoid proteinosis patient
Authors: F. Izadi F. Mahjoubi M. Farhadi M.M. Tavakoli S. Samanian
Lipoid proteinosis (LP) is a rare autosomal recessive disorder. Classical clinical features include warty skin infiltration, papules on the eyelids, skin scarring, as well as extracutaneous abnormalities such as hoarseness of the voice, epilepsy, and neuropsychiatric abnormalities. A defect in the ECM1 gene is responsible for this disease. A 21-year-old fema.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.14.2
- DOI:
- 10.4238/2012.November.14.2
Differential expression of the MHM region and of sex-determining-related genes during gonadal development in chicken embryos
Authors: L.C. Caetano, F.G.O. Gennaro, K. Coelho, F.M Ara�ºjo, R.A. Vila,A. Ara�ºjo, A. de Melo Bernardo, C.R. Marcondes, S.M. Chuva de Sousa Lopes, and E.S. Ramos
The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.13.2
- DOI:
- 10.4238/2014.February.13.2
A novel COL1A1 gene-splicing mutation (c.1875+1G>C) in a Brazilian patient with osteogenesis imperfecta
Authors: C. Barbirato M.G. Almeida M. Milanez V. Sipolatti M.R.G.O. Rebou�§as A.N. Akel Jr. V.R.R. Nunes A.M.S. Perrone M. Zatz I.D. Louro F. Paula
Osteogenesis imperfecta is a heterogeneous genetic disorder characterized by bone fragility and deformity, recurrent fractures, blue sclera, short stature, and dentinogenesis imperfecta. Most cases are caused by mutations in COL1A1 and COL1A2 genes. We present a novel splicing mutation in the COL1A1 gene (c.1875+1G>C) in a .. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr563
- DOI:
- 10.4238/vol8-1gmr563
Selection and validation of reference houseÃ?Âkeeping genes in the J774A1 macrophage cell line for quantitative real-time PCR
Authors: F.B. Ferraz and J.H. Fernandez
Macrophages are essential components of the innate and adaptive immune responses, playing a decisive role in atherosclerosis, asthma, obesity, and cancer. The differential gene expression resulting from adhesion of macrophages to the extra-cellular matrix (ECM) has been studied in the J774A1 murine macrophage cell line using quantitative polymerase chain .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017720
- DOI:
- 10.4238/gmr.15017720
Association between the epithelial cadherin -160C/A gene polymorphism and diffuse gastric cancer risk: a meta-analysis.
Authors: X.W. Chen, J.X. Sun, Z.N. Wang, P. Gao, Y.X. Song, J.F. Cao, B. Liu and H.M. Xu
Several previous studies have investigated whether the -160C/A epithelial cadherin promoter polymorphism confers an increased risk of diffuse gastric cancer (DGC), but conflicting results have been reported. To explore further the association of this polymorphism with DGC susceptibility, we performed an extensive search of rel.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.13.3
- DOI:
- http://dx.doi.org/10.4238/2014.February.13.3
Analysis of POU1F1 gene DdeI polymorphism in Chinese goats
Authors: M.J. Li1,2, C.M. Zhang2, X.Y. Lan1, X.T. Fang3, C.Z. Lei1 and H. Chen1
As a member of the POU-domain family, the POU1F1 is a positive regulator for growth hormone, prolactin and thyroid-stimulating hormone b, by binding to target DNA promoters as a dimer in mammals. This study described the polymorphisms at the goat POU1F1-DdeI locus and analyzed the distribution of alleles in 15 indigenous Chinese goat breeds. The PCR-RFLP .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017747
- DOI:
- 10.4238/gmr.15017747