Abstract

Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita

Author(s): S.P. Ling, S.S. Su, H.M. Zhang, X.S. Zhang, X.Y. Liu, G.F. Pan and Y. Yuan

Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this species. Using rapid amplification of cDNA ends technologies with 2 degenerate primers that were designed based on the hsp90 gene sequence from other members of Asteraceae, we isolated and characterized an Hsp90 homolog gene from M. recutita (Mr-Hsp90).

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