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Gene

Plant Genetics   Research Article

Regulation of bolting and identification of the �±-tubulin gene family in Brassica rapa L. ssp pekinensis

Authors: Y.W. Zhang1,2, D. Jin1, C. Xu1, L. Zhang1, M.H. Guo1 and Z.Y. Fang3

Microtubules are important components of eukaryotic cells, and they play vital roles in cell morphogenesis, carrying of signaling molecules, transport of materials, and establishing the cell polarity. During bolting of biennial plants, cell division and elongation are involved, and cell elongation inevitably involves the microtubules arrangement and expre.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017507
DOI:
10.4238/gmr.15017507
Animal Genetics   Research Article

Selection of an effective microsatellite marker system for genetic control and analysis of gerbil populations in China

Authors: X.Y. Du, W. Li, X.Y. Sa, C.L. Li, J. Lu, Y.Z. Wang and Z.W. Chen

Although gerbils have been widely used in many areas of biological research over many years, there is currently no effective genetic quality control system available. In the present study, we sought to establish a microsatellite marker system for quality control and conducted an optimized analysis of 137 microsatellite loci in two laboratory gerbil popula.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.21.16
DOI:
http://dx.doi.org/10.4238/2015.September.21.16
Medical Genetics   Research Article

Chemical synthesis and improved expression of recombinant human granulocyte colony-stimulating factor cDNA

Authors: S. Alrokayan

Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as a useful molecule for the treatment of a wide range of complex ailments, such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. The vast therapeutic potential of G-CSF has induced scientists to develop biotechnological approaches f.. Read More»

Genet. Mol. Res. 10(4):
2011.October.31.18
DOI:
10.4238/2011.October.31.18
Human Genetics   Research Article

Exhaustive search for conservation networks of populations representing genetic diversity

Authors: J.A.F. Diniz-Filho1, J.V.B.P.L. Diniz2 and M.P.C. Telles3

Conservation strategies routinely use optimization methods to identify the smallest number of units required to represent a set of features that need to be conserved, including biomes, species, and populations. In this study, we provide R scripts to facilitate exhaustive search for solutions that represent all of the alleles in networks with the smallest .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017525
DOI:
10.4238/gmr.15017525
Microbial Genetics   Research Article

Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains

Authors: F.A. Oliveira, K.S. Paludo, L.N.V.S. Arend, S.M.S.S. Farah, F.O. Pedrosa, E.M. Souza, M. Surek, G. Picheth and C.M.T. Fadel-Picheth

Eight virulence factors associated with uropathogenic Escherichia coli (UPEC) were investigated in 204 clinical isolates of E. coli recovered from urine cultures at counts ≥105. The bacteria were classified into two groups according to the number of leukocytes in urine samples from which they were isolated: group I ≤8 le.. Read More»

Genet. Mol. Res. 10(4):
2011.October.31.5
DOI:
10.4238/2011.October.31.5
Animal Genetics   Research Article

Genetic and environmental effects on age at first farrowing in sows in southeastern Brazil

Authors: A. Cavalcante-Neto, J.F. Lui, J.L.R. Sarmento, M.N. Ribeiro, C. Fonseca, H. Tonhati, J.M.C. Monteiro, M.A.C. Lara and S.A. Queiroz

We evaluated genetic and environmental factors affecting age at first farrowing of sows in the Brazilian southeast. For this purpose, 466 observations regarding the age at first farrowing were made for Dalland-C40© animals belonging to two herds. The effects of the environmental factors on this trait were assessed by mean.. Read More»

Genet. Mol. Res. 10(4):
2011.October.31.9
DOI:
10.4238/2011.October.31.9
Plant Genetics   Research Article

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Authors: A.C.P. Silveira, R.C. Antunes, J.F. Almeida, T.F. Braga, P.F.A. Freitas, A.S.M. C�©sar and E.C. Guimar�£es

The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fra.. Read More»

Genet. Mol. Res. 7(4):
vol7-4gmr511
DOI:
10.4238/vol7-4gmr511
Human Genetics   Research Article

Lack of association between catalase gene polymorphism (T/C exon 9) and susceptibility to vitiligo in a Turkish population

Authors: H. Bulut, M. Pehlivan, S. Alper, A.G. Tomatir, H. Onay, S.E. Y�¼ksel, F. ��zkinay and S. Pehlivan

Accumulation of hydrogen peroxide (H2O2) and low catalase (CAT) activity have been demonstrated in the epidermis of vitiligo patients. We investigated a possible association between the CAT exon 9 (Asp-389) gene and vitiligo susceptibility in the Turkish population. Thirty-four patients with vitiligo and 49 gender, age and eth.. Read More»

Genet. Mol. Res. 10(4):
2011.October.31.13
DOI:
10.4238/2011.October.31.13
Microbial Genetics   Research Article

A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene

Authors: M.J. Liu, G.M. Du, F.F. Bai, Y.Z. Wu, Q.Y. Xiong, Z.X. Feng, B. Liand G.Q. Shao

The aim of this study was to establish a method for sensitive and rapid diagnosis of Mycoplasma hyopneumoniae in clinical specimens. To this effect, we employed three sets of primers specifically designed for amplification of nucleic acids under isothermal conditions. After optimization of reaction conditions, M. hyopneumoniae could be successfully detect.. Read More»

Genet. Mol. Res. 14(2):
2015.May.4.27
DOI:
10.4238/2015.May.4.27
Human Genetics   Research Article

Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues

Authors: X.G. Han, D.K. Wang, F. Gao, R.H. Liu and Z.G. Bi

Bone morphogenetic protein 2 (BMP-2) can promote fracture healing. Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear. Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis. In this study, tissue composition and express.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.21.19
DOI:
http://dx.doi.org/10.4238/2015.September.21.19