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Gene

Animal Genetics   Research Article

Genetic diversity and assessment of 23 microsatellite markers for parentage testing of Santa In�ªs hair sheep in Brazil

Authors: C.A. Souza, S.R. Paiva, C.M. McManus, H.C. Azevedo, A.S. Mariante and D. Grattapaglia

Santa Inês is the most common hair sheep breed in Brazil and probably has the highest genetic diversity among sheep breeds in this country. Successful breeding programs for Brazilian sheep breeds are not common for various reasons, including a lack of control of parentage in the flocks. We developed an allele frequency d.. Read More»

Genet. Mol. Res. 11(2):
2012.May.8.4
DOI:
10.4238/2012.May.8.4
Human Genetics   Research Article

Comparative study of 13 candidate genes applying multi-reference normalization to detect the expression of different fineness in skin tissues of wool sheep

Authors: Y.Z. Tian, T. Usman, K.C. Tian, J. Di, X.X. Huang, X.M. Xu, H. Tulafu, W.W. Wu, X.F. Fu, Y. Bai, M. Tuerxun and A. Maimaiti

Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provid.. Read More»

Genet. Mol. Res. 16(1):
gmr16018905
DOI:
10.4238/gmr16018905
Plant Genetics   Research Article

Use of simple sequence repeat markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp) cultivars resistant and susceptible to red rot

Authors: U. Hameed, Y.-B. Pan, K. Muhammad, S. Afghan and J. Iqbal

Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers w.. Read More»

Genet. Mol. Res. 11(2):
2012.May.8.1
DOI:
10.4238/2012.May.8.1
Plant Genetics   Research Article

Development and validation of the first SSR markers for Mimosa scabrella Benth

Authors: F.A. Saiki, A.P. Bernardi, M.S. Reis, H. Faoro, E.M. Souza, F.O. Pedrosa, A. Mantovani and A.F. Guidolin

Mimosa scabrella Benth., popularly known as ‘‘bracatinga’’, is a pioneer and endemic species of Brazil, occurring in Mixed Ombrophilous Forest associated with Brazilian Atlantic Rainforest biomes. It is a fast-growing tree of the Fabaceae family that facilitates the dynamics of ecological succession. SS.. Read More»

Genet. Mol. Res. 16(1):
gmr16019571
DOI:
10.4238/gmr16019571
Human Genetics   Research Article

Frequency of alterations in the MEFV gene and clinical signs in familial Mediterranean fever in Central Anatolia, Turkey

Authors: G.G. Ceylan, C. Ceylan and E. Ozturk

Familial Mediterranean fever is a recessive autoinflammatory disease that is frequent in Armenians, Jews, Arabs, and Turks. The MEFV gene is responsible for this disease. We looked for MEFV gene variations (polymorphism and mutations) in a population that resides in Central Anatolia, Turkey. DNA was extracted from peripheral b.. Read More»

Genet. Mol. Res. 11(2):
2012.May.7.4
DOI:
10.4238/2012.May.7.4
Plant Genetics   Research Article

Chloroplast diversity of Casearia grandiflora in the Cerrado of Piau�­ State

Authors: M.F. Costa, A.A. Pereira, J.B. Pinheiro, M.I. Zucchi, A.S.F. Ara�ºjo, R.L.F. Gomes, S.E.S. Valente, M.E.A. Oliveira and A.C.A. Lopes

Casearia grandiflora (Salicaceae) is a typical Cerrado species adapted to disturbed environments, making it useful for restoration projects. Knowledge of genetic diversity is important for establishing conservation strategies for this species. This study aimed to compare chloroplast haplotype diversity and structure of C. gran.. Read More»

Genet. Mol. Res. 16(1):
gmr16019572
DOI:
10.4238/gmr16019572
Human Genetics   Research Article

Dysregulation of gene expression in a patient with depressive disorder after transient ischemic attack confirmed by a neurophysiological neuromarker

Authors: M. Trystu��a, M. �»ychowska, M. Wilk-Fra��czuk, J.D. Kropotov and M. P��chalska

The aim of this study was to evaluate dysregulation of gene expression associated with the cellular stress response in a patient with a post-“warning stroke” depressive disorder confirmed by the presence of a neurophysiological neuromarker through the use of quantitative EEG and event-related potentials. The patien.. Read More»

Genet. Mol. Res. 16(1):
gmr16019532
DOI:
10.4238/gmr16019532
Plant Genetics   Research Article

Genetic relationships in a germplasm collection of Camellia japonica and Camellia oleifera using SSR analysis

Authors: Y. Zhao, C.J. Ruan, G.J. Ding and S. Mopper

Camellia japonica produces different color and bigger flowers, widely being used for gardening green in southern China. However, cultivars were introduced from different regions, but their origin and pedigree information is either not available poorly documented, causing problems in authentication. Many low-yield trees in Came.. Read More»

Genet. Mol. Res. 16(1):
gmr16019526
DOI:
10.4238/gmr16019526
Plant Genetics   Research Article

Comparative evaluation of total RNA extraction methods in Theobroma cacao using shoot apical meristems

Authors: D.V. Silva1, S.M.J. Branco1, I.S.A. Holanda2, S. Royaert3, J.C. Motamayor4,J.P. Marelli4 and R.X. Corr�ªa5

Theobroma cacao is a species of great economic importance with its beans used for chocolate production. The tree has been a target of various molecular studies. It contains many polyphenols, which complicate the extraction of nucleic acids with the extraction protocols requiring a large amount of plant material. These issues, therefore, necessitate the op.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017364
DOI:
10.4238/gmr.15017364
Human Genetics   Research Article

CsSAD: a fatty acid desaturase gene involved in abiotic resistance in Camellia sinensis (L.)

Authors: Z.T. Ding1,2*, J.Z. Shen1,2*, L.L. Pan1,2, Y.U. Wang1,2, Y.S. Li3, Y. Wang4 andH.W. Sun5

Tea (Camellia sinensis L.) is a thermophilic evergreen woody plant that has poor cold tolerance. The SAD gene plays a key role in regulating fatty acid synthesis and membrane lipid fluidity in response to temperature change. In this study, full-length SAD cDNA was cloned from tea leaves using rapid amplification of cDNA ends and polymerase chain reaction .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017512
DOI:
10.4238/gmr.15017512