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Gene

Medical Genetics   Research Article

Analysis of the genetic diversity of beach plums by simple sequence repeat markers

Authors: X.M. Wang, W.L. Wu, C.H. Zhang, Y.P. Zhang, W.L. Li and T. Huang

The purpose of this study was to measure the genetic diversity of wild beach plum and cultivated species, and to determine the species relationships using SSRs markers. An analysis of genetic diversity from ten beach plum germplasms was carried out using 11 simple sequence repeat (SSR) primers selected from 35 primers to generate distinct PCR products. Fr.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.2
DOI:
10.4238/2015.August.19.2
Microbial Genetics   Research Article

In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops

Authors: C. Bredow, J.L. Azevedo, J.A. Pamphile, C.A. Mangolin and S.A. Rhoden

Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.3
DOI:
10.4238/2015.August.19.3
Plant Genetics   Research Article

Genetic diversity and structure of the threatened species Sinopodophyllum hexandrum (Royle) Ying

Authors: W. Liu, J. Wang, D.X. Yin, M. Yang, P. Wang, Q.S. Han, Q.Q. Ma, J.J. Liu and J.X. Wang

Sinopodophyllum hexandrum is an important medicinal plant that has been listed as an endangered species, making the conservation of its genetic diversity a priority. Therefore, the genetic diversity and population structure of S. hexandrum was investigated through inter-simple sequence repeat analysis of eight natural populati.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028130
DOI:
10.4238/gmr.15028130
Microbial Genetics   Research Article

Identification of target genes for adenohypophysis-prefer miR-7 and miR-375 in cattle

Authors: B. Yuan, G.J. Sun, G.L. Zhang, J. Wu, C. Xu, L.S. Dai, J. Chen, X.F. Yu, Z.H. Zhao and J.B. Zhang

In this study, expression levels of miRNAs (miRNAs), miR-375 and miR-7, were detected in different tissues of cattle to determine whether adenohypophysis-prefer or exclusively expressed miRNAs, and target genes could be predicted by TargetScan, RNA22, and other software. Target genes related to pituitary function or reproductive traits were identified usi.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.8
DOI:
10.4238/2015.August.19.8
Medical Genetics   Research Article

Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids

Authors: M. Cervantes, N. Arce, H. Garc�­a, M. Cota, J.K. Htoo and A. Morales

Excess Leu reduces the availability of Ile and Val in pigs likely by reducing absorption of the latter amino acids (AA). Twenty-four crossbred pigs were used to evaluate the effect of excess Leu alone or with surplus Ile and Val on the expression of cationic (b0,+AT and CAT1) and neutral (B0AT1) AA transporters in the small intestine, liver, and skeletal .. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.11
DOI:
10.4238/2015.August.19.11
Microbial Genetics   Research Article

Population structure and genetic diversity in Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae): is there a correlation between genetic and geographic proximity?

Authors: A.L.S. Mascarenhas, A.M. Waldschmidt and J.C. Silva Jr.

Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae) is a minuscule insect species, which forms galls, is subsocial, and parthenogenetic. It is associated with Ficus benjamina L. (Moraceae) and has a pantropical occurrence. The paucity of genetic studies on the order Thysanoptera led us to use inter-simple sequence repeat molecular marker to assess intra- .. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.12
DOI:
10.4238/2015.August.19.12
Human Genetics   Research Article

Genetic polymorphisms of loci D18S53, D18S59, and D18S488 in fetuses from a Chinese Tianjin Han population

Authors: X.Z. Li, J. Liu, Y.F. Shi, D. Ju, Y. Zhang and T.F. Yue

We investigated the genetic polymorphisms of three short tandem repeat (STR) loci, D18S53, D18S59, and D18S488, on chromosome 18 in fetuses from a Chinese Tianjin Han population. Sixty-four villus samples and 374 amniotic fluid samples were collected from fetuses. Quantitative fluorescence polymerase chain reaction was perform.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027820
DOI:
10.4238/gmr.15027820
Microbial Genetics   Research Article

Improving production of extracellular proteases by random mutagenesis and biochemical characterization of a serine protease in Bacillus subtilis S1-4

Authors: X.C. Wang, H.Y. Zhao, G. Liu, X.J. Cheng and H. Feng

The feather is a valuable by-product with a huge annual yield produced by the poultry industry. Degradation of feathers by microorganisms is a prerequisite to utilize this insoluble protein resource. To improve the degrading efficiency of feathers, mutagenesis of the bacterium Bacillus subtilis S1-4 was performed. By combining.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027831
DOI:
10.4238/gmr.15027831
Bioinformatics   Research Article

Isolation and characterization of eight novel microsatellite markers in Acanthopagrus schlegelii

Authors: X.Q. Mao, Z.B. Li, Y. Yuan, Y.F. Ning, J.B. Shangguan,Y.S. Huang, M Yang and B.B. Li

Acanthopagrus schlegelii is a warm temperate demersal fish, which inhabits the sediment substrate or rocky reefs in shallow seas. As this fish is a nutritionally endowed species with good palatability, it is a highly valuable commercial species for aquaculture and has a long historical standing in Western Pacific countries. Be.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027902
DOI:
10.4238/gmr.15027902
Plant Genetics   Research Article

Genetic and epigenetic alterations induced by different levels of rye genome integration in wheat recipient

Authors: X.L. Zheng, J.P. Zhou, L.L. Zang, A.T. Tang, D.Q. Liu, K.J. Deng and Y. Zhang

The narrow genetic variation present in common wheat (Triticum aestivum) varieties has greatly restricted the improvement of crop yield in modern breeding systems. Alien addition lines have proven to be an effective means to broaden the genetic diversity of common wheat. Wheat-rye addition lines, which are the direct bridge ma.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028001
DOI:
10.4238/gmr.15028001