Authors: Y. Zhou, S.-J. Teng, L. Yang, S.-B. Li and Y. Xu
The aim of this study was to investigate the existence of a β-lactamase gene in a group of multi-drug resistant Acinetobacter baumannii. Twenty strains of multi-drug resistant A. baumannii were isolated. Thirty-four β-lactamase genes and the ISaba1- OXA-23 linkage were analyzed in these strains by polymerase chain reaction (PCR) and verified by .. Read More»
Authors: M.H. Deng, J.L. Huo, H.S. Zhu, H. Zhou and J.F. Wen
We isolated two transcription factor genes, heterotrimeric G protein beta 2 subunit (Gβ2) and ArcA1, from pepper (Capsicum annuum). The complete coding sequences were amplified using reversed transcriptase PCR based on conserved sequence information of Solanum lycopersicum and several other plant species. Nucleotide sequence analysis of these two gen.. Read More»
Authors: D.-X. Chen, L.-Y. Li, X. Zhang, Y. Wang and Z. Zhang
Wild Dipsacus chinensis plants in China have become endangered owing to over-harvesting and habitat fragmentation. We examined the genetic diversity and genetic structure of 90 individuals from three populations using inter-simple sequence repeat markers and found that 106 of 173 bands amplified by 22 informative and reliable primers were polymorphic. The.. Read More»
Authors: C.P. Coelho, A.P. Costa Netto, J. Colasanti and A. Chalfun-Junior
Molecular analysis of floral induction in Arabidopsis has identified several flowering time genes related to 4 response networks defined by the autonomous, gibberellin, photoperiod, and vernalization pathways. Although grass flowering processes include ancestral functions shared by both mono- and dicots, they have developed their own mechanisms to transmi.. Read More»
Authors: S. Simsek, S. Tekes, D. Oral, A. Turkyilmaz, B. Isik, M.R. Isik and H. Akkoc
An insertion/deletion (I/D) polymorphism was identified in intron 16 of the gene encoding the human angiotensin I-converting enzyme (ACE), a candidate gene for chronic obstructive pulmonary disease (COPD). We investigated the relationship between this polymorphism in the ACE gene and the risk of developing COPD. Sixty-six COPD in-patients and 40 non-smoki.. Read More»
Authors: L. Chen, Y.Q. Chen, A.M. Ding, H. Chen, F. Xia, W.F. Wang and Y.H. Sun
The TCP family is a transcription factor family, members of which are extensively involved in plant growth and development as well as in signal transduction in the response against many physiological and biochemical stimuli. In the present study, 61 TCP genes were identified in tobacco (Nicotiana tabacum) genome. Bioinformatic.. Read More»
Authors: Z.B. Li, Y.F. Ning, J.B. Shangguan, B.B. Li, X.Q. Mao, Y.S. Huang and Y. Yuan
Ruditapes philippinarum is considered a commercially valuable species, which is commonly found in tidal flats along West Pacific coasts. In China, it is mainly distributed in the southeast sea. In this study, 16 novel microsatellite loci from the R. philippinarum genome were developed, using the protocol of fast isolation by a.. Read More»
Authors: R.F. Peixoto-Junior, S. Creste, M.G.A. Landell, D.S. Nunes, A. Sanguino, M.F. Campos, R. Vencovsky, E.V. Tambarussi and A. Figueira
Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the gen.. Read More»
Authors: Q. Li, T.F. Lu, D. Liu, P.F. Hu, B. Sun, J.Z. Ma, W.J. Wang, K.F. Wang, W.X. Zhang, J. Chen, W.J. Guan, Y.H. Ma and M.H. Zhang
The Amur tiger is a unique endangered species in the world, and thus, protection of its genetic resources is extremely important. In this study, an Amur tiger placenta cDNA library was constructed using the SMART cDNA Library Construction kit. A total of 508 colonies were sequenced, in which 205 (76%) genes were annotated and .. Read More»
Authors: M.S. Reynoso, C.M. Alvarez, L. De la Cruz, M.D. Escoto and J.J.G. S�¡nchez
Corn is a major crop and various herbicides are used to maximize its production, which include a dicamba-atrazine mixture. This has great advantages, but can also induce DNA damage. Genotoxic activity was assessed by comet assay following application of two concentrations of dicamba-atrazine: 1000-2000 and 2000-4000 ppm. Apical meristem leaf nuclei from 1.. Read More»