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Gene

Microbial Genetics   Research Article

A novel variant of the �²-lactamase ADC-61 gene in multi-drug resistant Acinetobacter baumannii

Authors: Y. Zhou, S.-J. Teng, L. Yang, S.-B. Li and Y. Xu

The aim of this study was to investigate the existence of a β-lactamase gene in a group of multi-drug resistant Acinetobacter baumannii. Twenty strains of multi-drug resistant A. baumannii were isolated. Thirty-four β-lactamase genes and the ISaba1- OXA-23 linkage were analyzed in these strains by polymerase chain reaction (PCR) and verified by .. Read More»

Genet. Mol. Res. 14(2):
2015.June.29.2
DOI:
10.4238/2015.June.29.2
Microbial Genetics   Research Article

Molecular cloning and tissue expression analyses of two novel pepper genes: heterotrimeric G protein beta 2 subunit and ArcA1

Authors: M.H. Deng, J.L. Huo, H.S. Zhu, H. Zhou and J.F. Wen

We isolated two transcription factor genes, heterotrimeric G protein beta 2 subunit (Gβ2) and ArcA1, from pepper (Capsicum annuum). The complete coding sequences were amplified using reversed transcriptase PCR based on conserved sequence information of Solanum lycopersicum and several other plant species. Nucleotide sequence analysis of these two gen.. Read More»

Genet. Mol. Res. 12(2):
2013.April.17.1
DOI:
10.4238/2013.April.17.1
Cytogenomics   Research Article

Genetic diversity in wild Dipsacus chinensis populations from China based on ISSR markers

Authors: D.-X. Chen, L.-Y. Li, X. Zhang, Y. Wang and Z. Zhang

Wild Dipsacus chinensis plants in China have become endangered owing to over-harvesting and habitat fragmentation. We examined the genetic diversity and genetic structure of 90 individuals from three populations using inter-simple sequence repeat markers and found that 106 of 173 bands amplified by 22 informative and reliable primers were polymorphic. The.. Read More»

Genet. Mol. Res. 12(2):
2013.April.12.7
DOI:
10.4238/2013.April.12.7
Cytogenomics   Research Article

A proposed model for the flowering signaling pathway of sugarcane under photoperiodic control

Authors: C.P. Coelho, A.P. Costa Netto, J. Colasanti and A. Chalfun-Junior

Molecular analysis of floral induction in Arabidopsis has identified several flowering time genes related to 4 response networks defined by the autonomous, gibberellin, photoperiod, and vernalization pathways. Although grass flowering processes include ancestral functions shared by both mono- and dicots, they have developed their own mechanisms to transmi.. Read More»

Genet. Mol. Res. 12(2):
2013.April.25.6
DOI:
10.4238/2013.April.25.6
Medical Genetics   Research Article

The insertion/deletion polymorphism in the ACE gene and chronic obstructive pulmonary disease

Authors: S. Simsek, S. Tekes, D. Oral, A. Turkyilmaz, B. Isik, M.R. Isik and H. Akkoc

An insertion/deletion (I/D) polymorphism was identified in intron 16 of the gene encoding the human angiotensin I-converting enzyme (ACE), a candidate gene for chronic obstructive pulmonary disease (COPD). We investigated the relationship between this polymorphism in the ACE gene and the risk of developing COPD. Sixty-six COPD in-patients and 40 non-smoki.. Read More»

Genet. Mol. Res. 12(2):
2013.April.25.10
DOI:
10.4238/2013.April.25.10
Plant Genetics   Research Article

Genome-wide analysis of TCP family in tobacco

Authors: L. Chen, Y.Q. Chen, A.M. Ding, H. Chen, F. Xia, W.F. Wang and Y.H. Sun

The TCP family is a transcription factor family, members of which are extensively involved in plant growth and development as well as in signal transduction in the response against many physiological and biochemical stimuli. In the present study, 61 TCP genes were identified in tobacco (Nicotiana tabacum) genome. Bioinformatic.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027728
DOI:
10.4238/gmr.15027728
Microbial Genetics   Research Article

Isolation and characterization of microsatellite markers in Ruditapes philippinarum

Authors: Z.B. Li, Y.F. Ning, J.B. Shangguan, B.B. Li, X.Q. Mao, Y.S. Huang and Y. Yuan

Ruditapes philippinarum is considered a commercially valuable species, which is commonly found in tidal flats along West Pacific coasts. In China, it is mainly distributed in the southeast sea. In this study, 16 novel microsatellite loci from the R. philippinarum genome were developed, using the protocol of fast isolation by a.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027742
DOI:
10.4238/gmr.15027742
Plant Genetics   Research Article

Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

Authors: R.F. Peixoto-Junior, S. Creste, M.G.A. Landell, D.S. Nunes, A. Sanguino, M.F. Campos, R. Vencovsky, E.V. Tambarussi and A. Figueira

Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the gen.. Read More»

Genet. Mol. Res. 13(3):
2014.September.26.23
DOI:
10.4238/2014.September.26.23
Animal Genetics   Research Article

Screening and analyzing genes associated with Amur tiger placental development

Authors: Q. Li, T.F. Lu, D. Liu, P.F. Hu, B. Sun, J.Z. Ma, W.J. Wang, K.F. Wang, W.X. Zhang, J. Chen, W.J. Guan, Y.H. Ma and M.H. Zhang

The Amur tiger is a unique endangered species in the world, and thus, protection of its genetic resources is extremely important. In this study, an Amur tiger placenta cDNA library was constructed using the SMART cDNA Library Construction kit. A total of 508 colonies were sequenced, in which 205 (76%) genes were annotated and .. Read More»

Genet. Mol. Res. 13(3):
2014.September.26.25
DOI:
10.4238/2014.September.26.25
Plant Genetics   Research Article

Evaluation of the genotoxic activity of dicamba and atrazine herbicides in several Mexican and South American varieties of sweetcorn (Zea mays L.)

Authors: M.S. Reynoso, C.M. Alvarez, L. De la Cruz, M.D. Escoto and J.J.G. S�¡nchez

Corn is a major crop and various herbicides are used to maximize its production, which include a dicamba-atrazine mixture. This has great advantages, but can also induce DNA damage. Genotoxic activity was assessed by comet assay following application of two concentrations of dicamba-atrazine: 1000-2000 and 2000-4000 ppm. Apical meristem leaf nuclei from 1.. Read More»

Genet. Mol. Res. 14(4):
2015.December.11.5
DOI:
10.4238/2015.December.11.5