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Gene

Plant Genetics   Research Article

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

Authors: S. Djebbi, D. Bouktila, H. Makni, M. Makni and M. Mezghani-Khemakhem1

Pea (Pisum sativum) is one of the most cultivated legumes in the world, and its yield and seed quality are affected by a variety of pathogens. In plants, NBS-LRR (nucleotide binding siteleucine-rich repeat) is the main class of disease resistance genes. Using degenerate primers deduced from conserved motifs in the NBS domain of known resistance genes, we .. Read More»

Genet. Mol. Res. 14(2):
2015.June.11.18
DOI:
10.4238/2015.June.11.18
Plant Genetics   Research Article

Study of the obp5 gene in Apis mellifera ligustica and Apis cerana cerana

Authors: H.X. Zhao, X.N. Zeng, Q. Liang, X.F. Zhang, W.Z. Huang, H.S. Chenand Y.X. Luo

Apis mellifera ligustica and A. cerana cerana exhibit differences in olfactory sensitivity to odors from nectariferous plants and diseased broods. It is presumed that the differences in odorant-binding proteins (OBPs) between these 2 species contribute to their olfactory sensitivity. We compared the sequences, temporal expression pattern, and binding prop.. Read More»

Genet. Mol. Res. 14(2):
2015.June.12.1
DOI:
10.4238/2015.June.12.1
Human Genetics   Research Article

Expression profiles of the apoptosis signaling pathway mediated by death receptor and endoplasmic reticulum in rat liver regeneration

Authors: X.K. Xing, M.H. Li, X.S. Zhu and C.S. Xu

The death receptor and endoplasmic reticulum (ER) are closely related to cell apoptosis, and it is worth studying whether the apoptosis pathways mediated by them are involved in liver regeneration. To understand the mechanism underlying death receptor- and ER-mediated apoptosis during rat liver regeneration, we used the Rat Ge.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028288
DOI:
10.4238/gmr.15028288
Plant Genetics   Research Article

Genetic diversity in elite inbred lines of maize and its association with heterosis

Authors: E.H. Fernandes, I. Schuster, C.A. Scapim, E.S.N. Vieira and M.M.D. Coan

The objective of the current study was to apply molecular markers (microsatellites) in the analysis of genetic diversity of 48 lines of the elite maize germplasm stored in the bank of the Cooperativa Central de Pesquisa Agrícola - Coodetec, PR, Brazil, and estimate the correlation between genetic distance and heterosis and hybrid performance from t.. Read More»

Genet. Mol. Res. 14(2):
2015.June.12.3
DOI:
10.4238/2015.June.12.3
Plant Genetics   Research Article

Molecular characterization of the myosatin gene and the effect of fasting on its expression in Chinese perch (Siniperca chuatsi)

Authors: L. Liu, Y.L. Li, S.D. Xu, K.Z. Wang, P. Wu, W.Y. Chu and X.Q. Wang

Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including .. Read More»

Genet. Mol. Res. 15(2):
gmr.15028354
DOI:
10.4238/gmr.15028354
Animal Genetics   Research Article

Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes

Authors: R.L. Yang, A.X. Wang, J. Zhang, Y. Dong, M.S. Yang and J.M. Wang

We characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. Two insect-resistant Bacillus thuringiensis (Bt) genes, Cry1Ac and Cry3A, and a salt-tolerant gene, Betaine aldehyde dehydrogenase (BADH) were inserted into a vector, p209-Cry1Ac-Cry3A-BADH. The clone of Populus x.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028635
DOI:
10.4238/gmr.15028635
Human Genetics   Research Article

Genetic structure of the threatened Hopea chinensis in the Quang Ninh Province, Vietnam

Authors: N.T.P. Trang and L. Triest

Hopea chinensis Hand-Mazz (synonym H. hongayensis Tardieu), is a wind and insect pollinated species. It is a threatened species known only from two locations: Quang Ninh (Vietnam) and Guangxi (China). As an endemic species, it is worth preserving both for dipterocarp biodiversity, as well as for its medicinal use and economic .. Read More»

Genet. Mol. Res. 15(2):
gmr.15028103
DOI:
10.4238/gmr.15028103
Medical Genetics   Research Article

Novel and recurrent COL7A1 mutations in Chinese patients with dystrophic epidermolysis bullosa pruriginosa

Authors: K.J. Zhu, C.Y. Zhu*, Y. Zhou and Y.M. Fan

Dystrophic epidermolysis bullosa pruriginosa (DEB-Pr) is a rare subtype of dystrophic epidermolysis bullosa (DEB). This disease is characterized by severe itching, lichenoid nodules or prurigo-like lesions, and linear scarring with a predilection for the extensor limbs. Pathogenic mutations in the type VII collagen alpha 1 (CO.. Read More»

Genet. Mol. Res. 13(3):
2014.September.12.26
DOI:
10.4238/2014.September.12.26
Human Genetics   Research Article

Reference gene selection for gene expression studies in lily using quantitative real-time PCR

Authors: M.F. Zhang, Q. Liu and G.X. Jia

Quantitative real-time polymerase chain reaction (qRT-PCR) is an important technology used to analyze gene-expression levels. Reference genes, which are assumed to be expressed consistently across various developmental stages and in different tissues, were selected for expression level analysis. Using digital gene expression t.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027982
DOI:
10.4238/gmr.15027982
Plant Genetics   Research Article

Characterization and genetic diversity of pepper (Capsicum spp) parents and interspecific hybrids

Authors: M.P.S.D. Costa, M.M. do R�ªgo, A.P.G. da Silva, E.R. do R�ªgo and P.A. Barroso

Pepper species exhibit broad genetic diversity, which enables their use in breeding programs. The objective of this study was to characterize the diversity between the parents of different species and their interspecific hybrids using morphological and molecular markers. The parents of Capsicum annuum (UFPB-01 and -137), C. ba.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027652
DOI:
10.4238/gmr.15027652