Gene
Molecular cloning and bioinformatic analysis of the Streptococcus agalactiae neuA gene isolated from tilapia
Authors: E.L. Wang, K.Y. Wang, D.F. Chen, Y. Geng, L.Y. Huang, J. Wangand Y. He
Cytidine monophosphate (CMP) N-acetylneuraminic acid (NeuNAc) synthetase, which is encoded by the neuA gene, can catalyze the activation of sialic acid with CMP, and plays an important role in Streptococcus agalactiae infection pathogenesis. To study the structure and function of the S. agalactiae neuA gene, we isolated it from diseased tilapia, amplified.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.18
- DOI:
- 10.4238/2015.June.1.18
Genetic diversity and differentiation in Camellia reticulata (Theaceae) polyploid complex revealed by ISSR and ploidy
Authors: B.-Y. Wang and Z.-Y. Ruan
Camellia reticulata is a well-known ornamental and oil plant that is endemic to southwest China. This species shows three cell ploidies, i.e., diploidy, tetraploidy and hexaploidy. We made the first investigation of genetic diversity and differentiation of natural populations of C. reticulata, and 114 individuals from 6 popula.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.6.3
- DOI:
- 10.4238/2012.March.6.3
Integrated miRNA-mRNA analysis of Epstein-Barr virus-positive nasopharyngeal carcinoma
Authors: L.H. Zhu, X.T. Miao and N.Y. Wang
This study aims to identify the crucial miRNAs in Epstein-Barr virus-positive nasopharyngeal carcinoma (NPC) and their target genes. Gene expression profile data (GSE12452) that included 31 NPC and 10 normal nasopharyngeal tissue specimens were downloaded. Differentially expressed genes (DEGs) were identified using significance analysis of microarrays. Th.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.20
- DOI:
- 10.4238/2015.June.1.20
A cytological study of anther and pollen development in Camellia oleifera
Authors: C. Gao, D.Y. Yuan, B.F. Wang, Y. Yang, D.M. Liu and Z.Q. Han
The plant species Camellia oleifera is an important producer of edible oil in China. However, it suffers from a low fruit-setting rate. This study used high resolution scanning electron microscopy of semi-thin tissue sections to investigate anther development and pollen formation, and to determine whether problems in forming functional pollen cause low se.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.24
- DOI:
- 10.4238/2015.July.31.24
Expression and antibody generation of the cancer-testis antigen, BIOT2-S
Authors: J.-Y. Wang, M. Cao, M.-R. Guo, S. Li, X.-F. Yang, M. Wang, J. Fang and J. Zhao
Biot2-S is a mouse cancer-testis antigen gene that was identified using the cross-reactive serological analysis of recombinant cDNA expression libraries (SEREX) technique in the State Key Laboratory of Biotherapy, West China Hospital, Sichuan University. To express BIOT2-S and generate its antibody for further investigation, the Biot2-S prokaryotic recomb.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.17
- DOI:
- 10.4238/2015.July.31.17
Correlation analysis between single nucleotide polymorphism of DRD1 gene and stereotyped behavior of blue fox
Authors: Z.Y. Liu, E.J. Ren, H.X. Ba, Q. Wu, H.W. Zhu, X.M. Xing and F.H. Yang
This study was performed to investigate the correlation between stereotyped behavior of the blue fox and single nucleotide polymorphisms (SNPs) of the DRD1 gene. We choose the DRD1 gene as a major gene for investigating the correlation of gene polymorphism and self-biting disease by means of direct sequencing. Part of the DRD1 gene exon of the blue fox wa.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.1
- DOI:
- 10.4238/2015.June.8.1
Novel SNPs in the growth arrest and DNA damage-inducible protein 45 alpha gene (GADD45A) associated with meat quality traits in Berkshire pigs
Authors: E.S. Cho, K.T. Lee, J.W. Choi, H.J. Jeon, S.W. Lee, Y.M. Cho and T.H. Kim
This study was conducted to evaluate the porcine gene GADD45A (growth arrest and DNA-damage-inducible protein 45 alpha) as a positional candidate controlling quantitative trait loci (QTL) for meat quality traits on chromosome 6 (SSC6). Four exons of the porcine GADD45A gene were defined from cDNA and BAC clone sequences. A total of 4 single nucleotide pol.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.6
- DOI:
- 10.4238/2015.July.31.6
Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs
Authors: Z.C. Wu, Y. Liu, Q.H. Zhao, S.P. Zhu, Y.J. Huo, G.Q. Zhu, S.L. Wuand W.B. Bao
The bactericidal/permeability-increasing protein (BPI) gene has been identified as a candidate gene for disease-resistance breeding. We evaluated whether polymorphisms in exons 4 and 10 of the BPI gene are associated with immune indices [interleukin-2 (IL- 2), IL-4, IL-6, interferon-b (IFN-b), IL-10, and IL-12]. In this study, we identified one mutation (.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.2
- DOI:
- 10.4238/2015.June.8.2
Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes
Authors: M. Guo, Y.L. Zhang, Z.J. Meng and J. Jiang
Micro-Tom is the smallest known variety of tomatoes. An orthogonal experimental design L16 (45) was used to optimize Agrobacterium-mediated transformation of cotyledon explants of Lycopersicon esculentum cv. Micro-Tom. Four parameters were investigated to determine their effect on transformation frequency: the concentration of.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.16.4
- DOI:
- 10.4238/2012.March.16.4
Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China
Authors: Z.-W. Chen, R.-J. Lu, L. Zou, Z.-Z. Du, R.-H. Gao, T. He and J.-H. Huang
We analyzed the genetic diversity of 115 barley germplasms, including 112 landraces and three new barley cultivars grown in the Shanghai region, using a set of 11 SSR markers. Sixty-six alleles were observed at the 11 SSR loci, ranged from three to ten, with a mean of six alleles per locus. The polymorphism information content ranged from 0.568 to 0.853, .. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.16.2
- DOI:
- 10.4238/2012.March.16.2