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Gene

Microbial Genetics   Research Article

Cloning and expression of the porcine attaching and effacing-associated (paa) gene of enteropathogenic Escherichia coli

Authors: D.A. Pereira, E.C. Teixeira Florian, M.A. Ono, C. Nachi Rossi, O. Vidotto and M.C. Vidotto

Porcine enteropathogenic Escherichia coli (PEPEC) produce an outer membrane protein (intimin) called Paa (porcine attaching and effacing-associated), which is involved in the pathogenesis of E. coli in piglets with diarrhea. The paa gene of a PEPEC strain isolated in Paraná, Brazil, was amplified by polymerase chain reaction, sequenced, and cloned .. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.5
DOI:
10.4238/2015.July.31.5
Plant Genetics   Research Article

Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers

Authors: I. Ullah, A. Iram, M.Z. Iqbal, M. Nawaz, S.M. Hasni and S. Jamil

The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence.. Read More»

Genet. Mol. Res. 11(1):
2012.March.14.3
DOI:
10.4238/2012.March.14.3
Microbial Genetics   Research Article

mRNA expression of prolactin receptor in sheep pituitary and its effect on maternal behavior

Authors: L.P. Wang

Prolactin receptor (PRLR) is the specific receptor for prolactin. Brain-specific control of PRLR gene expression is correlated with the induction of maternal behavior. The aim of this study was to investigate the dynamic changes in the PRLR mRNA level in the sheep pituitary gland and its effect on ewe maternal behavior. The mRNA expression of PRLR during .. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.13
DOI:
10.4238/2015.July.31.13
Animal Genetics   Research Article

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

Authors: C.H. Melo, F.C. Sousa, R.I.P.T. Batista, D.J.D. Sanchez, J.M.G. Souza-Fabjan, V.J.F. Freitas, L.M. Melo and D.I.A. Teixeira

The present study aimed to compare laparoscopic (LP) and ultrasound-guided (US) biopsy methods to obtain either liver or splenic tissue samples for ectopic gene expression analysis in transgenic goats. Tissue samples were collected from human granulocyte colony stimulating factor (hG-CSF)-transgenic bucks and submitted to real-time PCR for the endogenous .. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.16
DOI:
10.4238/2015.July.31.16
Human Genetics   Research Article

Sequence variants in the bovine PRDM16 gene associated with body weight in Chinese cattle breeds

Authors: J. Wang, C. Wang, R. Tian, Y.-Z. Huang, X.-S. Lai, X.-Y. Lan, J.-Q. Wang and H. Chen

As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms .. Read More»

Genet. Mol. Res. 11(1):
2012.March.22.5
DOI:
10.4238/2012.March.22.5
Bioinformatics   Research Article

Association analysis between SNPs in the 5'-flanking region of the chicken GRP78 gene, thermotolerance parameters, and tissue mRNA expression

Authors: L.N. Kong, D.X. Zhang, C.L. Ji, X.Q. Zhang and Q.B. Luo

Glucose-regulated protein 78 (GRP78) is a molecular chaperone in the endoplasmic reticulum and can be induced by different kinds of environmental and physiological stress. Thus far, the role of the GRP78 gene in thermotolerance in chickens has not been investigated. In the present study, we detected sequence variations in the 5ê�?�?�?�?-flanking reg.. Read More»

Genet. Mol. Res. 14(2):
2015.June.8.9
DOI:
10.4238/2015.June.8.9
Plant Genetics   Research Article

Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.)

Authors: M.F. Santos, K.J. Damasceno-Silva, M.A. Carvalhaes and P.S.C. Lima

The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using randomamplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD .. Read More»

Genet. Mol. Res. 14(2):
2015.June.8.10
DOI:
10.4238/2015.June.8.10
Plant Genetics   Research Article

Genetic variation in a wild population of the ��?sleep��? passion fruit (Passiflora setacea) based on molecular markers

Authors: C.B.M. Cerqueira-Silva, E.S.L. Santos, L.D.H.C.S. Concei�?§�?£o, C.B. Cardoso-Silva, A.S. Pereira, A.C. Oliveira and R.X. Corr�?ªa

Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the ‘sleep’ passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the comple.. Read More»

Genet. Mol. Res. 11(1):
2012.March.22.3
DOI:
10.4238/2012.March.22.3
Plant Genetics   Research Article

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Authors: Z. Noormohammadi, H. Samadi-Molayousefi and M. Sheidai

Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 .. Read More»

Genet. Mol. Res. 11(1):
2012.March.19.4
DOI:
10.4238/2012.March.19.4
Plant Genetics   Research Article

Combining ability of S3 progenies for key agronomic traits in popcorn: comparison of testers in top-crosses

Authors: V.J. de Lima, A.T. do Amaral Junior, S.H. Kamphorst, G.F. Pena,J.T. Leite, K.F.M. Schmitt, C. Vittorazzi, J.E. de Almeida Filho and F. Mora

The successful development of hybrid cultivars depends on the reliability of estimated combining ability of the parent lines. The objectives of this study were to assess the combining ability of partially inbred S3 families of popcorn derived from the open-pollinated variety UENF 14, via top-crosses with four testers, and to c.. Read More»

Genet. Mol. Res. 15(4):
gmr15049319
DOI:
10.4238/gmr15049319