Authors: D.A. Pereira, E.C. Teixeira Florian, M.A. Ono, C. Nachi Rossi, O. Vidotto and M.C. Vidotto
Porcine enteropathogenic Escherichia coli (PEPEC) produce an outer membrane protein (intimin) called Paa (porcine attaching and effacing-associated), which is involved in the pathogenesis of E. coli in piglets with diarrhea. The paa gene of a PEPEC strain isolated in Paraná, Brazil, was amplified by polymerase chain reaction, sequenced, and cloned .. Read More»
Authors: I. Ullah, A. Iram, M.Z. Iqbal, M. Nawaz, S.M. Hasni and S. Jamil
The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence.. Read More»
Authors: L.P. Wang
Prolactin receptor (PRLR) is the specific receptor for prolactin. Brain-specific control of PRLR gene expression is correlated with the induction of maternal behavior. The aim of this study was to investigate the dynamic changes in the PRLR mRNA level in the sheep pituitary gland and its effect on ewe maternal behavior. The mRNA expression of PRLR during .. Read More»
Authors: C.H. Melo, F.C. Sousa, R.I.P.T. Batista, D.J.D. Sanchez, J.M.G. Souza-Fabjan, V.J.F. Freitas, L.M. Melo and D.I.A. Teixeira
The present study aimed to compare laparoscopic (LP) and ultrasound-guided (US) biopsy methods to obtain either liver or splenic tissue samples for ectopic gene expression analysis in transgenic goats. Tissue samples were collected from human granulocyte colony stimulating factor (hG-CSF)-transgenic bucks and submitted to real-time PCR for the endogenous .. Read More»
Authors: J. Wang, C. Wang, R. Tian, Y.-Z. Huang, X.-S. Lai, X.-Y. Lan, J.-Q. Wang and H. Chen
As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms .. Read More»
Authors: L.N. Kong, D.X. Zhang, C.L. Ji, X.Q. Zhang and Q.B. Luo
Glucose-regulated protein 78 (GRP78) is a molecular chaperone in the endoplasmic reticulum and can be induced by different kinds of environmental and physiological stress. Thus far, the role of the GRP78 gene in thermotolerance in chickens has not been investigated. In the present study, we detected sequence variations in the 5ê�?�?�?�?-flanking reg.. Read More»
Authors: M.F. Santos, K.J. Damasceno-Silva, M.A. Carvalhaes and P.S.C. Lima
The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using randomamplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD .. Read More»
Authors: C.B.M. Cerqueira-Silva, E.S.L. Santos, L.D.H.C.S. Concei�?§�?£o, C.B. Cardoso-Silva, A.S. Pereira, A.C. Oliveira and R.X. Corr�?ªa
Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the ‘sleep’ passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the comple.. Read More»
Authors: Z. Noormohammadi, H. Samadi-Molayousefi and M. Sheidai
Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 .. Read More»
Authors: V.J. de Lima, A.T. do Amaral Junior, S.H. Kamphorst, G.F. Pena,J.T. Leite, K.F.M. Schmitt, C. Vittorazzi, J.E. de Almeida Filho and F. Mora
The successful development of hybrid cultivars depends on the reliability of estimated combining ability of the parent lines. The objectives of this study were to assess the combining ability of partially inbred S3 families of popcorn derived from the open-pollinated variety UENF 14, via top-crosses with four testers, and to c.. Read More»