Gene
Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.)
Authors: L.-L. Guan, W. Wu, B. Hu, D. Li, J.-W. Chen, K. Hou and L. Wang
Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.7
- DOI:
- 10.4238/2014.August.28.7
Chromosomal localization and partial sequencing of the 18S and 28S ribosomal genes from Bradysia hygida (Diptera: Sciaridae)
Authors: V.P. Gaspar, E.L.T. Shimauti and M.A. Fernandez
In insects, ribosomal genes are usually detected in sex chromosomes, but have also or only been detected in autosomal chromosomes in some cases. Previous results from our research group indicated that in Bradysia hygida, nucleolus organizer regions were associated with heterochromatic regions of the autosomal C chromosome, usi.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.26.6
- DOI:
- 10.4238/2014.March.26.6
Locally linear embedding and neighborhood rough set-based gene selection for gene expression data classification
Authors: L. Sun, J.-C. Xu, W. Wang and Y. Yin
Cancer subtype recognition and feature selection are important problems in the diagnosis and treatment of tumors. Here, we propose a novel gene selection approach applied to gene expression data classification. First, two classical feature reduction methods including locally linear embedding (LLE) and rough set (RS) are summar.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038990
- DOI:
- 10.4238/gmr.15038990
Building the SeqChromMM Markov property atlas of the human genome by analyzing the 200-bp units of the 15 different chromatin regions of ENCODE
Authors: K.-E. Lee and H.-S. Park
We analyzed the publicly available ChromHMM BED files of the ENCODE project and tested the Markov properties of the different chromatin states in the human genome. Nucleotide frequency profiles of regional chromatin segmentations were analyzed, and Markov chains were built to detect Markov properties in the chromatin states of different ChromHMM regions. .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038992
- DOI:
- 10.4238/gmr.15038992
Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase
Authors: H. Liu, R. Ye and Y.Y. Wang
Gene mutation plays an important role in molecular biological studies. A highly efficient one-step polymerase chain reaction-based mutagenesis technique for site-directed mutagenesis was developed in this study. One complementary pair of primers was designed that contained the desired mutations in the middle of the primers. The amplification products of m.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.10
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.10
Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines
Authors: J. Ogorevc and P. Dov?
Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.12
Regulators of G-protein signaling 9 genetic variations in Chinese subjects with schizophrenia
Authors: Y.S. Zhu, Y.X. Li, X.M. Qiao and H.B. Zhang
To identify single-nucleotide polymorphisms that contribute to the genetic susceptibility to schizophrenia, we examined the potential association between schizophrenia and 9 single nucleotide polymorphisms (rs1530351, rs4791230, rs2869577, rs8077696, rs8070231, rs2292592, rs9916525, rs1122079, and rs4790953) in the G-protein signaling 9 gene. The particip.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.13
- DOI:
- 10.4238/2015.July.28.13
Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA
Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.20
- DOI:
- 10.4238/2015.July.28.20
Fragmentation effects and genetic diversity of the key semidecidual forest species Metrodorea nigra in Southwestern Brazil
Authors: R.M. Moraes Filho, F. Bonif?¡cio-Anacleto and A.L. Alzate-Marin
Studies of genetic diversity in plant species present in the remaining fragments of the Atlantic Forest are very important for understanding their resilience to such a degraded ecosystem. We analyzed the genetic diversity of 3 populations of the high-density understory species Metrodorea nigra St. Hill. (Rutaceae) located in forest remnants in the region .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.15
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.15
Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers
Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang
Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.7
- DOI:
- 10.4238/2015.July.28.7