Gene
Isolation and characterization of novel microsatellite markers in commercial selected golden Malaysian arowana fish, Scleropages formosus (Osteoglossidae)
Authors: B. Manoharan, Z. Sulaimen, F. Omar, R.Y. Othman, S.Z. Mohamed and S. Bhassu
Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr944
- DOI:
- 10.4238/vol10-2gmr944
Resistance of soybean genotypes to Sclerotinia sclerotiorum isolates in different incubation environments
Authors: L.H.S. Castro, A.A. Figueir�³, A.P.O. Nogueira, S.J. Clough and F.C. Juliatti
Sclerotinia sclerotiorum is an important soybean pathogen. The objectives of this study were to evaluate levels of resistance of soybean genotypes to the fungus, and to determine the effects of different incubation environments on host resistance and pathogen aggressiveness. Two experiments were conducted using 103 genotypes f.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049061
- DOI:
- 10.4238/gmr15049061
Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages
Authors: M.Q. Zhang, C.A. Ma, W.W. Lv, Y.H. Huang, D.L. Wang and Y.L. Zhao
To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.10
- DOI:
- 10.4238/2015.June.1.10
Determination of the optimal number of markers and individuals in a training population necessary for maximum prediction accuracy in F2 populations by using genomic selection models
Authors: L.A. Peixoto, L.L. Bhering and C.D. Cruz
Genomic selection is a useful technique to assist breeders in selecting the best genotypes accurately. Phenotypic selection in the F2 generation presents with low accuracy as each genotype is represented by one individual; thus, genomic selection can increase selection accuracy at this stage of the breeding program. This study.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048874
- DOI:
- 10.4238/gmr15048874
DNA barcoding and phylogenetic relationships in Timaliidae
Authors: Z.H. Huang and D.H. Ke
The Timaliidae, a diverse family of oscine passerine birds, has long been a subject of debate regarding its phylogeny. The mitochondrial cytochrome c oxidase subunit I (COI) gene has been used as a powerful marker for identification and phylogenetic studies of animal species. In the present study, we analyzed the COI barcodes of 71 species from 21 genera .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.11
- DOI:
- 10.4238/2015.June.1.11
Polymorphisms of the cocaine-amphetamine-regulated transcript (CART) gene and their association with reproductive traits in Chinese goats
Authors: P.Q. Wang, L.M. Deng, B.Y. Zhang, M.X. Chu and J.Z. Hou
Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorp.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1091
- DOI:
- 10.4238/vol10-2gmr1091
Population structure and genetic variation of the endangered species Elaeagnus mollis Diels (Elaeagnaceae)
Authors: J.M. Zhang and F. Zhang
Elaeagnus mollis Diels is a group of shrubs and dwarf trees endemic to China and are endangered plants. However, the reason why these plants are endangered remains controversial. The current study aimed to explore the endangered status of E. mollis from a genetic perspective and to propose conservation strategies for this species. Using 16 polymorphic all.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.12
- DOI:
- 10.4238/2015.June.1.12
Genetic relationships analysis of olive cultivars grown in China
Authors: M.M. Zhan, Z.Z. Cheng, G.C. Su, A.Y. Wang, H.P. Chen, Z.S. Yang, Z. Shan and Q.M. Huang
The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISS.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.13
- DOI:
- 10.4238/2015.June.1.13
Genetic variability of Myzus persicae nicotianae densovirus based on partial NS and VP gene sequences
Authors: X.R. Song, S.H. Tang, Z.Q. Tang, X.M. Yang, X.W. Wang, X.F. Wang, P.J. Xu and G.W. Ren
We previously described a novel densovirus [Myzus persicae nicotianae densovirus (MpnDV)] infecting M. persicae nicotianae (Hemiptera: Aphididae) with 34% prevalence. This single-stranded DNA virus has a 5480-nucleotide ambisense genome and belongs to the Densovirinae subfamily within the family Parvoviridae. In the present st.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049099
- DOI:
- 10.4238/gmr15049099
Genetic diversity among coffee tree progenies Big Coffee VL based on growth traits and production
Authors: J.A. Silva, S.P. Carvalho, A.T. Bruzi, R.J. Guimar�£es, L.L. Oliveira and L.C. Sim�µes
In a coffee plantation of a coffee ‘Acaiá’ cultivar (Coffea arabica), on the Midwest of Minas Gerais in Capitólio city, a different kind of coffee tree was found (1989), possibly due to a mutation. It presented larger leaves and grains than those of conventional coffee trees and was named as “Big Coffee VL.” The aim o.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049066
- DOI:
- 10.4238/gmr15049066