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Gene

Animal Genetics   Research Article

Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae)

Authors: R.L.M. Arakaki, H.V. Souza, M.M.U. Castanhole, H.E.M.C. Bicudo and M.M. Itoyama

The nucleolar material of Chariesterus armatus was analyzed during spermiogenesis in cell preparations impregnated with silver nitrate. Nucleolar corpuscles were observed in spermatids at the beginning of the process, showing that this organoid is also maintained after meiosis. In addition, nucleoli were seen in the round sper.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr930
DOI:
10.4238/vol9-3gmr930
Medical Genetics   Research Article

EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis

Authors: E. Galeano, A.A.M. Barroso, T.S. Vasconcelos, A. L�³pez-Rubio, A.J.P. Albrecht, R. Victoria Filho and H. Carrer

Weed resistance to herbicides is a natural phenomenon that exerts selection on individuals in a population. In Brazil, glyphosate resistance was recently detected in Digitaria insularis. The objective of this study was to elucidate mechanisms of weed resistance in this plant, including genetic variability, allelism, amino acid substitutions, gene expressi.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038730
DOI:
10.4238/gmr.15038730
Animal Genetics   Research Article

Targeted gene therapy and in vivo bioluminescent imaging for monitoring postsurgical recurrence and metastasis in mouse models of liver cancer

Authors: Q. He, C.L. Yao, L. Li, Z. Xin, Z.K. Jing and L.X. Li

We investigated the effects of combined targeted gene therapy on recurrence and metastasis after liver cancer resection in nude mice. Twenty BALB/C mice were randomly divided into control and treatment groups with 10 mice in each group and a male/female ratio of 1:1. Luciferase gene-labeled human primary hepatic carcinoma cell line MHCC97-H was then used .. Read More»

Genet. Mol. Res. 15(3):
gmr.15037878
DOI:
10.4238/gmr.15037878
Plant Genetics   Research Article

Analysis of �Fuji� apple somatic variants from next-generation sequencing

Authors: H.S. Lee1, G.H. Kim1, S.I. Kwon2, J.H. Kim2, Y.S. Kwon2 and C. Choi1

The domesticated apple (Malus x domestica Borkh.) is a major fruit crop of temperate regions of the world. ‘Fuji’ apple (Ralls Genet x Delicious), a famous apple cultivar in Korea, has been very popular since its promotion in Japan in 1958. ‘Fuji’ and its bud mutant cultivars possess variable levels of genetic diversity. Nonetheles.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038185
DOI:
10.4238/gmr.15038185
Animal Genetics   Research Article

Random regression analysis of test-day milk yields in the first and second lactations of Brazilian Gyr cows

Authors: L.G. Gonzalez-Herrera, L. El Faro, A.B. Bignardi, R.J. Pereira4, C.H.C. Machado and L.G. Albuquerque

The objective of the present study was to estimate the genetic parameters for test-day milk yields (TDMY) in the first and second lactations using random regression models (RRM) in order to contribute to the application of these models in genetic evaluation of milk yield in Gyr cattle. A total of 53,328 TDMY records from 7118 lactations of 5853 Gyr cows w.. Read More»

Genet. Mol. Res. 14(4):
2015
DOI:
10.4238/2015
Plant Genetics   Research Article

Characterization of maize genotypes for genetic diversity on the basis of inter simple sequence repeats

Authors: R.W. Muhammad, A. Qayyum, M.Q. Ahmad, A. Hamza, M. Yousaf, B. Ahmad, M. Younas, W. Malik, S. Liaqat, E. Noor

Genetic diversity in crops is essential to make improvements related to superior germplasms. Implementation of molecular markers to identify suitable genotypes speeds up the breeding progress by enhancing selection efficiency. This study was carried out to probe genetic diversity among 21 maize genotypes using 20 inter simple .. Read More»

Genet. Mol. Res. 16(1):
gmr16019438
DOI:
10.4238/gmr16019438
Animal Genetics   Research Article

Evaluation of perfused bovine udder for gene expression studies in dairy cows

Authors: I.S.B. Pinto, I. Fonseca, H.M. Brand�£o, J.C. Gern3, A.S. Guimar�£es, W.A. Carvalho, M.A.V.P. Brito, L.F. Viccini, M.F. Martins

Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfuse.. Read More»

Genet. Mol. Res. 16(1):
gmr16019637
DOI:
10.4238/gmr16019637
Plant Genetics   Research Article

Genetic and molecular characterization of multiflorous spikelet in oat

Authors: C.M. Zimmer, I.P. Ubert, K. Pellizzaro, L.C. Federizzi, I.C. Nava

Multiflorous spikelets are found in several grass species of agricultural and economic interest. In oat, this morphological characteristic is associated with the production of naked grains. Although many genetic studies have been performed over the past century, the inheritance of the multiflorous spikelet trait is not fully u.. Read More»

Genet. Mol. Res. 16(1):
gmr16019588
DOI:
10.4238/gmr16019588
Plant Genetics   Research Article

Fengshui forests conserve genetic diversity: a case study of Phoebe bournei (Hemsl.) Yang in southern China

Authors: Y.J. Ge, Y.J. Liu, A.H. Shen and X.C. Lin

Fengshui forests (sacred groves) are important in traditional Chinese culture and home to many endangered species. These forests may provide protection for some endangered plant species outside the nature reserves, but little is known about their role in genetic conservation. Using inter-simple sequence repeat (ISSR) markers, we compared the genetic diver.. Read More»

Genet. Mol. Res. 14(1):
2015.March.20.8
DOI:
10.4238/2015.March.20.8
Human Genetics   Research Article

TNFR1 -383 AÃ?Â?C polymorphism and ankylosing spondylitis in a Russian Caucasian population: a preliminary study

Authors: V. Mordovskii, A. Semenchukov, S.Y. Nikulina, A.B. Salmina, A. Chernova, E. Kapustina, A. Kents, A. Ohapkina, E. Moskaleva, P.E. Maltese, P. Convertini, M. Bertelli

The aim of this study was to assess the association between the TNFR1 rs2234649 polymorphism and ankylosing spondylitis susceptibility in a Russian Caucasian population. A total of 41 ankylosing spondylitis patients and 43 healthy controls, matched according to age and sex, were enrolled, and polymerase chain reac.. Read More»

Genet. Mol. Res. 16(1):
gmr16019581
DOI:
10.4238/gmr16019581