Gene
Expression differences in TEL-AML1 fusion gene in leukemia glucocorticoid-sensitive and -resistant cell lines
Authors: X.W. Wang and Y.H. Xu
We investigated the expression differences of the TEL-AML1 fusion gene in a leukemia glucocorticoid (GC)-sensitive cell line (CEM) and a GC-resistant cell line (Jurkat). Changes in TEL-AML1 expression before and after GC exposure were analyzed. Expression of GC-sensitive and GC-resistant leukemia cells following initial diagnosis and during treatment was .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.14
- DOI:
- 10.4238/2015.July.14.14
Association between genetic polymorphisms of PTGS2 and glioma in a Chinese population
Authors: R.P. Lin, C.Y. Yao and D.X. Ren
Several previous studies indicated that genetic polymorphisms in inflammatory factor genes were associated with glioma risk. However, the relationship between the prostaglandin-endoperoxide synthase 2 (PTGS2) genetic polymorphism and glioma remains unclear in the Chinese population. We selected 199 histologically confirmed adult glioma patients and 199 ca.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.25
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.25
Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango
Authors: C. Luo, H.X. Wu, Q.S. Yao, S.B. Wang and W.T. Xu
Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.17
- DOI:
- 10.4238/2015.July.14.17
Comparisons of DNA marker-based genetic diversity with phenotypic estimates in maize grown in Pakistan
Authors: M.M. Shah, S.W. Hassan, K. Maqbool, I. Shahzadi and A. Pervez
We compared DNA-based genetic diversity estimates with conventional estimates by investigating agronomically important traits in maize grown in the northwestern region of Pakistan. RAPD markers were used to characterize 10 commonly cultivated maize genotypes. The same material was tested for phenotypic variation of quantitativ.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr964
- DOI:
- 10.4238/vol9-3gmr964
Occurrence of natural triploidy in Rhamdia quelen (Siluriformes, Heptapteridae)
Authors: J.R. Tsuda, V.P.O. de Moraes, L. Giuliano-Caetano and A.L. Dias
Five specimens of Rhamdia quelen collected from the Lindóia Stream, PR, Brazil, were cytogenetically analyzed. The diploid chromosome number found was 58, including 30 metacentric, 16 submetacentric, 10 subtelocentric, and 2 acrocentric chromosomes. Supernumerary or B chromosomes, frequently observed in this fish group,.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr949
- DOI:
- 10.4238/vol9-3gmr949
Genetic progress resulting from forty-three years of breeding of the carioca common bean in Brazil
Authors: L.D. Barili, N.M. Vale, L.M. Moura, R.G. Paula, F.F. Silva and J.E.S. Carneiro
We aimed to evaluate 40 common bean cultivars recommended by various Brazilian research institutions between 1970 and 2013 and estimate the genetic progress obtained for grain yield and other agronomic traits. Additionally, we proposed a bi-segmented nonlinear regression model to infer the year in which breeding began to show significant gains in Brazil. .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038523
- DOI:
- 10.4238/gmr.15038523
Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa
Authors: B.L. Huang, X.K. Zhang, Y.Y. Li, D.Y. Li, M.Y. Ma, D.T. Cai, W.H. Wu and B.Q. Huang
Eruca vesicaria subsp sativa is one of the most tolerant Cruciferae species to drought, and dehydration-responsive element-binding protein 2A (DREB2A) is involved in responses to salinity, heat, and particularly drought. In this study, a gene encoding EvDREB2A was cloned and characterized in E. vesicaria subsp sativa. The full-length EvDREB2A cDNA sequenc.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038540
- DOI:
- 10.4238/gmr.15038540
Cut-and-paste-based cloning strategy for large gene site-directed mutagenesis
Authors: C. Wang, T.Y. Wang, L.Y. Zhang, X.J. Gao, X.W. Wang and C.J. Jin
Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be perf.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.10
- DOI:
- 10.4238/2015.May.25.10
Lack of association between SNP rs3914132 of the RELN gene and otosclerosis in India
Authors: S. Priyadarshi, K.C. Panda, A.K. Panda and P.V. Ramchander
Otosclerosis (MIM 166800) is primarily a metabolic bone disorder of the otic capsule, which leads to bony fixation of the stapedial footplate in the oval window; it is among the most common causes of acquired hearing loss. The etiology of this disease is largely unknown, although epidemiological studies suggest the involvement.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr890
- DOI:
- 10.4238/vol9-3gmr890
Proteomic and gene expression analyses during bolting-related leaf color change in Brassica rapa
Authors: Y.W. Zhang, M.H. Guo, X.B. Tang, D. Jin and Z.Y. Fang
Bolting and flowering are key processes during the growth and development of Chinese cabbage (Brassica rapa L. ssp pekinensis). Understanding the molecular mechanisms underlying bolting and flowering is of significance for improving production of the vegetable. A leaf-color change from bright green to gray-green has been observed following differentiation.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038584
- DOI:
- 10.4238/gmr.15038584