Gene
Molecular analysis of apomixis in cassava
Authors: N.M.A. Nassar, D.C. Kalkmann and R. Collevatti
Cassava is the main staple for more than 800 million people in the tropics. It is propagated vegetatively by stem cuttings, which maintains superior genotypes but favors disease accumulation and spread. In this report, we present the results of the screening of the progeny and the second generation of the clone UnB 307 for apo.. Read More»
- Genet. Mol. Res. 5(3):
Identification of non-specific alkaline phosphatases in hyphal cells of the fungus Neurospora crassa by in situ histochemistry
Authors: G. Thedei Junior and A. Rossi
The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three nonspecific alkaline phosphatase activities, one of them possibly be.. Read More»
- Genet. Mol. Res. 5(3):
DGAT1 K232A polymorphism in Brazilian cattle breeds
Authors: G.A. Lacorte, M.A. Machado, M.L. Martinez, A.L. Campos, R.P. Maciel, R.S. Verneque, R.L. Teodoro, M.G.C.D. Peixoto, M.R.S. Carvalho and C.G. Fonseca
Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds a.. Read More»
- Genet. Mol. Res. 5(3):
Identification and mapping of resistance genes to Phakopsora pachyrhizi in soybean (Glycine max L.) accession PI 594767-A
Authors: G.A.F. Rocha, D.P. Alves, J.C. Oliveira and S.H. Brommonschenkel
The goal of this study was to study resistance inheritance in the soybean (Glycine max L.) accession PI 594767-A to the Phakopsora pachyrhizi isolate PPUFV02, and map the resistance gene(s) identified using microsatellite markers. Crosses between PI 594767-A and the susceptible cultivar ‘Conquista’ gave rise to the segregating subpopulations 2.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038475
- DOI:
- 10.4238/gmr.15038475
Effects of forkhead box C2 on carcinogenesis and lymphatic metastasis in endometrial carcinoma
Authors: Y.Y. Fan, W.G. Deng, Y.N. Liu, Y.Y. Li, S.L. Deng and Y. Fu
We investigated the distribution of endometrial lymphatic vessels and expression of forkhead box C2 (FOXC2) in normal endometrium during menstrual cycle and in endometrial adenocarcinoma. Full-thickness uterine samples and endometrial adenocarcinoma samples were collected for immunohistochemical analysis using D2-40 and FOXC2 mouse monoclonal antibodies. .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.5
- DOI:
- 10.4238/2015.May.25.5
Mapping QTLs for drought tolerance in an F2:3 population from an inter-specific cross between Gossypium tomentosum and Gossypium hirsutum
Authors: J.Y. Zheng, G. Oluoch, M.K. Riaz Khan, X.X. Wang, X.Y. Cai, Z.L. Zhou, C.Y. Wang, Y.H. Wang, X.Y. Li, F. Liu and K.B. Wang
Cotton is one of the most important natural fiber crops in the world. Its growth and yield is greatly limited by drought. A quantitative trait locus (QTL) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (Gossypium spp) using 188 F2:3 lines developed from an inter-specific cross between a wild cotton species.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038477
- DOI:
- 10.4238/gmr.15038477
Decreased risk of developing lung cancer in subjects carrying the CLPTM1L rs401681 (G>A) polymorphism: evidence from a meta-analysis
Authors: X.L. Zhang, X.J. Zhang, Y. Chen, Y.M. Zhang, Q. Zhang,C.X. Cao, D.Y. Gu, J.F. Shi, Y.L. Gong, J.F. Chen and C.J. Tang
A genome-wide association study revealed that a single nucleotide polymorphism, CLPTM1L - rs401681 (G>A), located at the 5p15.33 locus was signifi cantly associated with increased risk of various cancers; however, its association with lung cancer is currently inconclusive. In order to explore the relationship between this p.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.28.10
- DOI:
- http://dx.doi.org/10.4238/2014.February.28.10
Heterogeneity and diversity of ABO and Rh blood group genes in select Saudi Arabian populations
Authors: E.S. AlSuhaibani, N.A. Kizilbash and S. Malik
In order to investigate the diversity of ABO and Rh blood group genes in the Saudi Arabian population, we assembled the phenotypic data of approximately 66,000 subjects from ten representative Saudi populations: Al-Khobar, Riyadh, Tabuk/Madina Al-Munawaara, Jeddah, Abha, South region, Sakaka, Domah, Al-Qurayat, and Sweer. The frequencies of p[A], q[B], an.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.11
- DOI:
- 10.4238/2015.July.14.11
Leptin and leptin receptor gene polymorphisms are correlated with production performance in the Arctic fox
Authors: M. Zhang and X.J. Bai
The polymerase chain reaction-single-strand conformation polymorphism technique was employed to measure mononucleotide diversity in the coding region of the leptin and leptin receptor genes in the Arctic fox. The relationships between specific genetic mutations and reproductive performance in Arctic foxes were determined to improve breeding strategies. We.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.7
- DOI:
- 10.4238/2015.May.25.7
Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes
Authors: F.V.M. Rubatino, N.V. Carobin, M.L. Freitas, V.T. de Oliveira, R.X. Pietra, P.P.R. Oliveira, A.A. Bosco and F.S. Jehee
High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, prime.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.12
- DOI:
- 10.4238/2015.July.14.12