Dna
Mitochondrial genome differences between the stingless bees Melipona rufiventris and Melipona mondury (Apidae: Meliponini)
Authors: Barni GS, Strapazzon R, Guerra Jr. JCV and Moretto G
Within the Meliponini, a widely distributed group of stingless bees, Melipona rufiventris has been considered as a single, cohesive species. Recently, analysis of morphological characters led to the splitting of this species into two species, M. mondury and M. rufiventris. The former occurs in the Atlantic Rain Forest ranging .. Read More»
- Genet. Mol. Res. 6(1):
Molecular study of Astyanax altiparanae (Osteichthyes, Characidae) as a probable species complex
Authors: I.C. Depr�¡, V.N. Gomes, G.C. Depr�¡, I.J. Oliveira, S.M.A.P. Prioli and A.J. Prioli
Astyanax altiparanae, belonging to the bimaculatus group, which includes species with similar colors and morphology, occurs in the upper Paraná River basin. As the use of mitochondrial DNA has made great strides in the diagnosis of species, in previous researches, two strains were detected in A. altiparanae.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.16
- DOI:
- 10.4238/2014.August.7.16
Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa
Authors: S.C.O. Melo, C. Pungartnik, J.C.M. Cascardo and M. Brendel
DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell d.. Read More»
- Genet. Mol. Res. 5(4):
Assessment of phylogenetic relationship of rare plant species collected from Saudi Arabia using internal transcribed spacer sequences of nuclear ribosomal DNA
Authors: F. Al-Qurainy, S. Khan, M. Nadeem, M. Tarroum and A. Alaklabi
The rare and endangered plants of any country are important genetic resources that often require urgent conservation measures. Assessment of phylogenetic relationships and evaluation of genetic diversity is very important prior to implementation of conservation strategies for saving rare and endangered plant species. We used internal transcribed spacer se.. Read More»
- Genet. Mol. Res. 12(1):
- 2013
- DOI:
- 10.4238/2013
The use of the acridine orange test and the TUNEL assay to assess the integrity of freeze-dried bovine spermatozoa DNA
Authors: Martins CF, Dode MN, Bao SN and Rumpf R
The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferasemediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. Th.. Read More»
- Genet. Mol. Res. 6(1):
Genetic variability in species of bats revealed by RAPD analysis
Authors: P.R.L. Moreira and E. Morielle-Versute
Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by .. Read More»
- Genet. Mol. Res. 5(4):
Associations of DNA polymorphisms in growth hormone and its transcriptional regulators with growth and carcass traits in two populations of Brangus bulls
Authors: Thomas MG, Enns RM, Shirley KL, Garcia MD, Garrett AJ, and Silver GA
Sequence polymorphisms in the growth hormone (GH) gene and its transcriptional regulators, Pit-1 and Prop-1, were evaluated for associations with growth and carcass traits in two populations of Brangus bulls Chihuahuan Desert Rangeland Research Center (CDRRC, N = 248 from 14 sires) and a cooperating breeding program (COOP, N =.. Read More»
- Genet. Mol. Res. 6(1):
Comparative analysis of different DNA extraction protocols in fresh and herbarium specimens of the genus Dalbergia
Authors: Ribeiro RA and Lovato MB
Five published DNA extraction protocols were compared for their ability to produce good quality DNA from fresh and herbarium leaves of several species of the genus Dalbergia. The leaves of these species contain high amounts of secondary metabolites, which make it difficult to perform a clean DNA extraction and thereby interfer.. Read More»
- Genet. Mol. Res. 6(1):
Screening for fragile X syndrome among Brazilian mentally retarded male patients using PCR from buccal cell DNA
Authors: D.M. Christofolini, M.V.N. Lipay, M.A.P. Ramos, D. Brunoni and M.I. Melaragno
Fragile X syndrome is one of the most frequent causes of mental retardation. Since the phenotype in this syndrome is quite variable, clinical diagnosis is not easy and molecular laboratory diagnosis is necessary. Usually DNA from blood cells is used in molecular tests to detect the fragile X mutation which is characterized by .. Read More»
- Genet. Mol. Res. 5(3):
Evaluation of DNA polymorphisms involving growth hormone relative to growth and carcass characteristics in Brahman steers
Authors: V.R. Beauchemin, M.G. Thomas, D.E. Franke2 and G.A. Silver
Associations of DNA polymorphisms in growth hormone (GH) relative to growth and carcass characteristics in growing Brahman steers (N = 324 from 68 sires) were evaluated. Polymorphisms were an Msp-I RFLP and a leucine/valine SNP in the GH gene as well as a Hinf-I RFLP and a histidine/arginine SNP in transcriptional regulators o.. Read More»
- Genet. Mol. Res. 5(3):