Dna

Analysis of DNA polymerase activity in vitro using non-radioactive primer extension assay in an automated DNA sequencer

Authors: Lopes DO, Regis-da-Silva CG, Machado-Silva A, Macedo AM, Franco GR, Hoffmann JS, Cazaux C, Pena SDJ, Teixeira SMR and Machado CR

Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used routinely for many years for DNA sequ.. Read More»

Genet. Mol. Res. 6(2):

Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines

Authors: E.M. Santos, J.F.R. Paula, P.M.C. Motta, M.B. Heinemann, R.C. Leite, J.P.A. Haddad, H.L. Del Puerto and J.K.P. Reis

We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using β-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to.. Read More»

Genet. Mol. Res. 9(3):

vol9-3gmr818

DOI:

10.4238/vol9-3gmr818

Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene

Authors: Q.Q. Song, Z.X. Chai, J.W. Xin, S.J. Zhao, Q.M. Ji, C.F. Zhang, Z.J. Ma and J.C. Zhong

To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide .. Read More»

Genet. Mol. Res. 14(1):

2015.March.13.3

DOI:

10.4238/2015.March.13.3

A multiplex panel of short-amplicon insertion-deletion DNA polymorphisms for forensic analysis

Authors: V.R.D. Santos, H.B. Pena and S.D.J. Pena

We have previously developed a panel of 40 insertion-deletion (INDEL) human DNA polymorphisms that was proven to adequately cover the span of global human genetic diversity. The panel was found to have very low matching probabilities with respect to both the global and Brazilian populations. To optimize the panel for application with degraded DNA samples,.. Read More»

Genet. Mol. Res. 14(2):

http://dx.doi.org/2015.April.10.2

DOI:

http://dx.doi.org/10.4238/2015.April.10.2

Germline HVR-II mitochondrial polymorphisms associated with breast cancer in Tunisian women

Authors: B. Yacoubi Loueslati, W. Troudi, L. Cherni, K.B. Rhomdhane and L. Mota-Vieira

A high incidence of somatic mtDNA polymorphisms has been reported in a wide variety of human cancers; some of them have been proposed as markers for the early detection of breast cancer. However, little attention has been paid to the potential of germline mitochondrial sequence variations as genetic risk factors for cancer. We.. Read More»

Genet. Mol. Res. 9(3):

vol9-3gmr778

DOI:

10.4238/vol9-3gmr778

DNA barcoding for efficient identification of Ixiolirion species (Ixioliriaceae)

Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang

Ixiolirion is a genus of unresolved taxonomy. DNA barcoding is a technique that allows species identification using standardized DNA sequences. In this study, a total of 23 individuals, representing 2 Chinese Ixiolirion species, were sampled to test the effectiveness of 3 DNA barcodes [internal transcribed spacer (ITS), chlorop.. Read More»

Genet. Mol. Res. 14(1):

2015.March.13.19

DOI:

10.4238/2015.March.13.19

A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon

Authors: G. Meru, D. McDowell, V. Waters, A. Seibel, J. Davis and C. McGregor

Genet. Mol. Res. 12(1):

Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers

Authors: S. Nawaz, F.A. Khan, S. Tabasum, M. Zakria, A. Saeed and M.Z. Iqbal

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dend.. Read More»

Genet. Mol. Res. 9(3):

vol9-3gmr903

DOI:

10.4238/vol9-3gmr903

Complete mitochondrial genome of a natural triploid crucian carp mutant, Carassius auratus var. pingxiangnensis, and phylogenetic analysis of different ploidies in crucian carp

Authors: J.Q. Sheng, J.H. Wang, S.H. He, L.G. Zeng, K. Peng and Y.J. Hong

Carassius auratus var. pingxiangnensis is a natural triploid crucian carp mutant. In order to understand its placement and genetic background at the gene level, the characteristics of mitochondrial DNA sequences and phylogenetic relationship were examined. The results showed that the mitochondrial DNA is a ci.. Read More»

Genet. Mol. Res. 13(3):

2014.August.1.3

DOI:

10.4238/2014.August.1.3

Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.

Authors: G.Q. Wen, J. Li, X.H. Liu, Y.S. Zhang and S.S. Wen

Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (R.. Read More»

Genet. Mol. Res. 13(1):

http://dx.doi.org/2014.February.28.6

DOI:

http://dx.doi.org/10.4238/2014.February.28.6