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Dna

Cytogenomics   Research Article

Worldwide diversity of the Y-chromosome tetra-local microsatellite DYS464

Authors: F.S.G. Kehdy and S.D.J. Pena

Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 r.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr968
DOI:
10.4238/vol9-3gmr968
Medical Genetics   Research Article

Molecular identification of Amazonian stingless bees using polymerase chain reaction single-strand conformation polymorphism

Authors: M.T. Souza and G.A. Carvalho-Zilse

In countries containing a mega diversity of wildlife, such as Brazil, identifying and characterizing biological diversity is a continuous process for the scientific community, even in face of technological and scientific advances. This activity demands initiatives for the taxonomic identification of highly diverse groups, such.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.4
DOI:
10.4238/2014.July.25.4
Microbial Genetics   Research Article

Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens

Authors: J.C. Polonio, T.T. Almeida, A. Garcia, G.E.G. Mariucci, J.L. Azevedo, S.A. Rhoden and J.A. Pamphile

Mikania glomerata (Spreng.), popularly known as “guaco”, is a plant from the Asteraceae family that has many therapeutic properties. The use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. Microorganism-host complex interactions are rel.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.5
DOI:
10.4238/2015.July.3.5
Microbial Genetics   Research Article

Isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source

Authors: L.A. Cassa-Barbosa, R.E.L. Proc�³pio, I.T.S.R. Matos and S.A. Filho

Few yeasts have shown the potential to efficiently utilize hemicellulosic hydrolyzate as the carbon source. In this study, microorganisms isolated from the Manaus region in Amazonas, Brazil, were characterized based on their utilization of the pentoses, xylose, and arabinose. The yeasts that showed a potential to assimilate these sugars were selected for .. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.28.12
DOI:
http://dx.doi.org/10.4238/2015.September.28.12
Plant Genetics   Research Article

Molecular characterization of Ephedra species found in Pakistan

Authors: S. Ghafoor, M.M. Shah, H. Ahmad, Z.A. Swati, S.H. Shah, A. Pervez and U. Farooq

Ephedra, also known as “ma huang”, is a dioecious, drought- and frost-resistant, perennial, evergreen shrub with compelling medicinal value. The genus is represented by 42 species around the world, 9 of which were provisionally reported from Pakistan. Species of the genus have a controversial taxonomy due to their .. Read More»

Genet. Mol. Res. 6(4):
Plant Genetics   Research Article

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Authors: X. Cao, Z. Wu, R. Zhou, F.L. Jiang and Z.E. Yang

Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could .. Read More»

Genet. Mol. Res. 14(1):
2015.March.6.11
DOI:
10.4238/2015.March.6.11
Animal Genetics   Research Article

Quantitative changes in mitochondrial DNA copy number in various tissues of pigs during growth

Authors: Y.M. Xie, L. Jin, X.J. Chen, M.N. He, Y. Wang, R. Liu, M.Z. Li and X.W. Li

Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult.. Read More»

Genet. Mol. Res. 14(1):
2015.March.6.12
DOI:
10.4238/2015.March.6.12
Human Genetics   Research Article

Sex-biased gene flow in African Americans but not in American Caucasians

Authors: Goncalves VF, Prosdocimi F, Santos LS, Ortega JM and Pena SDJ

We have previously shown evidence of strong sex-biased genetic blending in the founding and ongoing history of the Brazilian population, with the African and Amerindian contribution being highest from maternal lineages (as measured by mitochondrial DNA) and the European contribution foremost from paternal lineages (estimated f.. Read More»

Genet. Mol. Res. 6(2):
Plant Genetics   Research Article

Lack of interpopulation genetic structure in the genus Stegastes (Perciformes) with indication of local introgression

Authors: E.A. Dias Jr., A.S. Souza and W.F. Molina

The family Pomacentridae comprises about 326 species belonging to 28 genera. The genus Stegastes is composed of nearly 33 species, and 8 are endemic to the Brazilian Province, inhabiting the Brazilian coast (Stegastes fuscus, S. variabilis, S. leucosticus, S. uenfi, and S. pictus) or Western Atlantic oceanic islands (S. trinda.. Read More»

Genet. Mol. Res. 6(4):
Plant Genetics   Research Article

Evaluation of five protocols for DNA extraction from leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris

Authors: K. Aubakirova1,2, M. Omasheva1, N. Ryabushkina1, T. Tazhibaev2,G. Kampitova2 and N. Galiakparov1

Leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality DNA extraction performance. In this study, five extraction protocols were compared for their ability to produce good quality DNA from fresh and dried (with silica gel) leaves of t.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.February.27.13
DOI:
http://dx.doi.org/10.4238/2014.February.27.13