New genomic resources for the honey bee(Apis mellifera L.): development of a deep-coverage BAC library and a preliminary STC database
Authors: J.P. Tomkins, M. Luo, G.C. Fang1, D. Main1, J.L. Goicoechea,M. Atkins, D.A. Frisch, R.E. Page, E. Guzm�¡n-Novoa5, Y. Yu,G. Hunt6 and R.A. Wing
We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size o.. Read More»
- Genet. Mol. Res. 1(4):
Cassava, Manihot esculenta Crantz, genetic resources: origin of the crop, its evolution and relationships with wild relatives
Authors: N.M.A. Nassar
About 98 species of Manihot are known. All of them are native to the New World and are concentrated in four regions in Brazil and Central America. All the Manihot species so far examined have 2n = 36 chromosomes. Interspecific hybrids between cassava and its wild relatives show relatively normal meiosis, and further generation.. Read More»
- Genet. Mol. Res. 1(4):
(Co)variance components, genetic parameters and annual trends for calf weights in a Brahman herd kept on floodable savanna
Authors: Dieter Plasse1, Omar Verde, Jes�ºs Arango, Luis Camaripano, Hugo Fossi, Rafael Romero, Carlos Rodriguez M. and Jos�© L. Rumbos
(Co)variance components and genetic parameters were estimated for body weights of an elite Brahman herd under a designed, supervised management and genetic program, including strategic artificial insemination (AI). Restricted maximum likelihood methods were used with a univariate animal model for birth weight (BW) and a bivari.. Read More»
- Genet. Mol. Res. 1(4):
Ectodermal dysplasias (group A): elaborationof a computerized database to help withgenetic-clinical diagnosis(Displasias ectodÃ?©rmicas do grupo A:elaboraÃ?§Ã?£o de um banco de dadosinformatizado para auxÃ?Âlio ao diagnÃ?³sticogenÃ?©tico-clÃ?Ânico)
Authors: Toni Lisboa Costa
Ectodermal dysplasias are conditions that involve structures derived from the ectoderm, such as the epidermis and its enclosures, constituting a large and complex nosologic group, composed of rare entities with genetic etiology (in their majority). Freire-Maia, in 1971 and 1977, proposed a clinical definition and a clinical-mn.. Read More»
- Genet. Mol. Res. 1(3):
Inheritance of resistance to Meloidogyne incognita race 2 in the hot pepper cultivar Carolina Cayenne(Capsicum annuum L.)
Authors: Fausto de Souza-Sobrinho, Wilson Roberto Maluf, Luiz A.A. Gomes and Vicente Paulo Campos
Root-knot nematodes of the genus Meloidogyne are important pathogens affecting vegetable crop production in Brazil and worldwide. The pepper species Capsicum annuum includes both hot and sweet peppers; very little emphasis has been placed on breeding sweet peppers for nematode resistance. We report on the inheritance of resist.. Read More»
- Genet. Mol. Res. 1(3):
Molecular detection of XO - Turner syndrome
Authors: Carlos Alberto Longui, Mylene Neves Rocha,Liana Carla Albuquerque Peres Martinho,Gustavo Gir Gomes, Ricardo Eustachio de Miranda,Thomas Alves de Souza Lima, M�´nica Barbosa Melo and Osmar Monte
Turner syndrome is caused by haploinsufficiency of the short arm of X-chromosome, and is usually diagnosed by karyotyping. This procedure is time-consuming, expensive and unfeasible for population screening. We propose molecular detection of 45XO Turner patients based on the ability of HpaII, a methylation sensitive endonuclea.. Read More»
- Genet. Mol. Res. 1(3):
Greater genetic variability in Argentine Creole than in Thoroughbred horses based on serum protein polymorphisms
Authors: S. DÃ?Âaz, F.N. Dulout and P. Peral-GarcÃ?Âa
Genetic polymorphism was analyzed for five blood proteins: albumin - Al, esterase - Es, α1B-glycoprotein - Xk, transferrin - Tf and hemoglobin - Hb in 200 Thoroughbred (TB) and 124 Argentine Creole (AC) horses. Of the five systems examined, Tf and Hb were not in Hardy-Weinberg equilibrium in either breed and Es was not i.. Read More»
- Genet. Mol. Res. 1(3):
Micronucleus investigation of alcoholic patients with oral carcinomas
Authors: Andr�©a Ramirez, Pedro Henrique Saldanha
The micronucleus test (MN) is used as an indicator of genotoxic exposition, since it is associated with chromosome aberrations. An increased mutation rate in oral squamous cells, indicated by an increased MN frequency, is also related to the development of oral carcinomas. We evaluated the frequencies of MN and other metanucle.. Read More»
- Genet. Mol. Res. 1(3):
CENP-B is a conserved gene among vegetal
Authors: Olga Barbosa-Cisneros and Rafael Herrera-Esparza
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a .. Read More»
- Genet. Mol. Res. 1(3):
Frequencies of GSTM1, GSTT1, and GSTP1 polymorphisms in a Brazilian population
Authors: Ana Rossini, Davy C.M. Rapozo, LÃ?Âdia M.F. Amorim, Jacyara M.B. Macedo1, Raquel Medina, JosÃ?© F.N. Neto, ClÃ?¡udia V.M. Gallo and Luis F.R. Pinto
The glutathione S-transferase (GST) family of enzymes has a vital role in phase II of biotransformation of environmental carcinogens, pollutants, drugs and other xenobiotics. GSTs are polymorphic, with the type and frequency of polymorphism being ethnic dependent. Polymorphisms in GST genes have been shown to be associated wit.. Read More»
- Genet. Mol. Res. 1(3):