GAL4 causes developmental defects and apoptosis when expressed in the developing eye of Drosophila melanogaster
Authors: Jamie M. Kramer, Brian E. Staveley
The UAS/GAL4 ectopic expression system is widely used in Drosophila melanogaster for the overexpression of transgenes. This system operates under the assumption that the yeast transcription factor, GAL4, is inactive in D. melanogaster. Thus, GAL4 can be expressed under the control of D. melanogaster-specific promoters with lit.. Read More»
- Genet. Mol. Res. 2(1):
The influence of brood comb cell size on the reproductive behavior of the ectoparasitic mite Varroa destructor in Africanized honey bee colonies
Authors: Giancarlo A. Piccirillo, D. De Jong
Africanized honey bees (Apis mellifera, Hymenoptera: Apidae) in Brazil are tolerant of infestations with the exotic ectoparasitic mite, Varroa destructor (Mesostigmata: Varroidae), while the European honey bees used in apiculture throughout most of the world are severely affected. Africanized honey bees are normally kept in hi.. Read More»
- Genet. Mol. Res. 2(1):
Association between diabetes type 1 and DQB1* alleles in a case-control study conducted in Montevideo, Uruguay
Authors: Adriana Mimbacas, Francisco P�?©rez-Bravo, Pedro C. Hidalgo, Gerardo Javiel, Carmen Pisciottano, Rosario Grignola, Ana Mar�?a Jorge, Juan Pablo Gallino, Jackeline Gasagoite, Horacio Cardoso
We studied HLA DQB1 allele frequencies and the relative risk (RR) of various genotypes in 72 type 1 diabetic patients and 40 control individuals in Uruguay. This is a tri-racial (Caucasian, Black and Indo-American) mixed population. The products of the polymerase chain reaction amplifications were hybridized with oligonucleoti.. Read More»
- Genet. Mol. Res. 2(1):
Cell biology, molecular embryology, Lamarckian and Darwinian selection as evolvability
Authors: H. Hoenigsberg
The evolvability of vertebrate systems involves various mechanisms that eventually generate cooperative and nonlethal functional variation on which Darwinian selection can operate. It is a truism that to get vertebrate animals to develop a coherent machine they first had to inherit the right multicellular ontogeny. The ontogen.. Read More»
- Genet. Mol. Res. 2(1):
Comparing data on the reproduction of Varroa destructor
Authors: Maria H. Corr�?ªa-Marques, Luis Medina Medina, Stephen J. Martin, D. De Jong
Varroa destructor reproductive success is considered an important character for determining the resistance of honey bees to this mite parasite. However, most of the published data are not comparable due to the different methods of ascertaining and reporting reproduction. A recently published technique that involves reconstruct.. Read More»
- Genet. Mol. Res. 2(1):
RAPD analysis of herbicide-resistant Brasilian rice lines produced via mutagenesis
Authors: S.S. Sandhu, C�?¢ndido R. Bastos, Luiz Ernesto Azini, Augusto Tulmann Neto, Carlos Colombo
Over the last two decades, mutational techniques have become one of the most important tools available to progressive rice- breeding programs. In a mutation-breeding program initiated in 1999 at the Instituto Agronômico of Campinas, SP, Brazil, a rice line, IAC103, was selected for mutational studies with gamma radiation.. Read More»
- Genet. Mol. Res. 1(4):
Intraspecific variability of Bipolaris sorokiniana isolates determined by random-amplified polymorphic DNA (RAPD)
Authors: Aida T.S. Matsumura, Ariano M. Prestes, Sueli T. Van Der Sand, Andr�?©ia M.R. de Oliveir
Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original .. Read More»
- Genet. Mol. Res. 1(4):
Molecular characterization of ABC transporter-encoding genes in Aspergillus nidulans
Authors: Adriana Mendes do Nascimento, Maria Helena S. Goldman, Gustavo H. Goldman
As a preliminary step towards characterizing genes encoding ATP-binding cassette (ABC) transporters that confer pleiotropic drug resistance in Aspergillus, we used a PCR-based approach to isolate four DNA fragments corresponding to different ABC type transporter genes. DNA sequencing and Southern blot analysis confirmed that t.. Read More»
- Genet. Mol. Res. 1(4):
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin
Authors: S.M.Z. Di Mauro, L.R. Furlan, M.I.T. Ferro, M. Macari andJ.A. Ferro
The effects of breed and of recombinant bovine somatotropin (rbST) treatment on growth hormone gene expression were studied in young bulls. The experiment was completely randomized in a [2 x 2]-factorial arrangement, using two levels of rbST (0 or 250 mg/animal/14 days), and two breed groups (Nelore and Simmental x Nelore cros.. Read More»
- Genet. Mol. Res. 1(4):
No evidence for minority male mating advantage in wild type strains of Drosophila ananassae tested in multiple-choice experiments
Authors: Arundhati Som and Bashisth N. Singh
Minority male mating advantage was tested in wild type strains of Drosophila ananassae through multiple-choice experiments. Mating success of two types of flies present in five different ratios was scored by direct observation in an Elens-Wattiaux mating chamber. We found no evidence for minority male mating advantage in wild .. Read More»
- Genet. Mol. Res. 1(4):