Bacteriophages and insertion sequences of Chromobacterium violaceum ATCC 12472
Authors: Rosana de Almeida, Peterson Beltramini Trevilato, Luciana Aparecida Bartoleti, Jos�© Luiz Proen�§a-M�³dena, Ebert Seixas Hanna, Gustavo Bueno Gregoracci, Marcelo Brocchi
A fluid genome is a great advantage to prokaryotes, enabling quick adaptation to various types of ecological niches and to diverse environmental selective pressures. A substantial portion of these sudden changes is mediated by lateral gene transfer (LGT), through genetic recombination mechanisms, such as transformation, conjug.. Read More»
- Genet. Mol. Res. 3(1):
A new set of bioinformatics tools for genome projects
Authors: Luiz G.P. Almeida, Roger Paix�£o, Rangel C. Souza, Gisele C. da Costa, Darcy F. de Almeida, Ana T.R. de Vasconcelos
A new tool called System for Automated Bacterial Integrated Annotation - SABIA (SABIÁ being a very well-known bird in Brazil) was developed for the assembly and annotation of bacterial genomes. This system performs automatic tasks of assembly analysis, ORFs identification/analysis, and extragenic region analyses. Genome.. Read More»
- Genet. Mol. Res. 3(1):
Occupational genotoxicity risk evaluation through the comet assay and the micronucleus test
Authors: M.G. Martino-Roth, J. Vi�©gas, D.M. Roth
The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exf.. Read More»
- Genet. Mol. Res. 2(4):
Behavioral genetics of Drosophila ananassae
Authors: Shree Ram Singh, Bashisth N. Singh
Drosophila ananassae has a unique status among Drosophila species because of certain peculiarities in its genetic behavior. The most unusual feature of this species is its relatively high frequency of spontaneous male recombination. The results of studies on non-sexual behavior, such as phototactic responses, eclosion rhythm, .. Read More»
- Genet. Mol. Res. 2(4):
Preferred amino acids and thermostability
Authors: S�¡vio T. Farias, Maria Christina M. Bonato
Most organisms grow at temperatures from 20 to 50°C, but some prokaryotes, including Archaea and Bacteria, are capable of withstanding higher temperatures, from 60 to >100°C. Their biomolecules, especially proteins, must be sufficiently stable to function under these extreme conditions; however, the basis for thermo.. Read More»
- Genet. Mol. Res. 2(4):
Putative pyrophosphate phosphofructose 1-kinase genes identified in sugar cane may be getting energy from pyrophosphate
Authors: S.J. Suzuki, M.A. Mutton, J.M. Pizauro, M. I.T. Ferro, M. V.F. Lemos, M. J.R. Mutton, S. M.Z. Di Mauro
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have identified both the α and β subunits of this e.. Read More»
- Genet. Mol. Res. 2(4):
Evolution without speciation but with selection: LUCA, the Last Universal Common Ancestor in Gilbert�s RNA world
Authors: Hugo Hoenigsberg
This is not an attempt to analyze the Last Universal Common Ancestor (LUCA) to understand the origin of living systems. We do not know what came before Gilberts’ RNA world. Our analysis starts with the RNA world and with genes (biological replicators alla Dawkings) made up of RNA proteins with enzymatic catalytic functio.. Read More»
- Genet. Mol. Res. 2(4):
Genetic polymorphism at spinocerebellar ataxia 1 and 2 loci in Brazil
Authors: Stenio F.P. Duarte, Raquel S. Gestinari, M�¡rio Campos-Jr, M�¡rcia M.G. Pimentel, Marcelo A. Costa Lima
Dynamic mutation involves the expansion of a tandem arrayed DNA sequence that is polymorphic in the population. This mechanism is associated with neurological/neuromuscular disorders and the pathology depends on the extension of the repeated tract, with a specific threshold for each disease. We made a PCR-based characterizatio.. Read More»
- Genet. Mol. Res. 2(4):
Oxidative stress in Lactococcus lactis
Authors: Anderson Miyoshi, Tatiana Rochat, Jean-Jacques Gratadoux, Yves Le Loir, Sergio Costa Oliveira, Philippe Langella Vasco Azevedo
Lactococcus lactis, the most extensively characterized lactic acid bacterium, is a mesophilic- and microaerophilic-fermenting microorganism widely used for the production of fermented food products. During industrial processes, L. lactis is often exposed to multiple environmental stresses (low and high temperature, low pH, hig.. Read More»
- Genet. Mol. Res. 2(4):
Gene flow between cassava, Manihot esculenta Crantz, and wild relatives
Authors: N.M.A. Nassar
Controlled and natural hybridization between cassava and wild relatives does occur. Barriers within the genus appear to be weak due to recent evolution of the group. All Manihot species examined cytogenetically have a chromosome number of 2n = 36. However, they behave meiotically as diploids. The weak interspecific barriers ha.. Read More»
- Genet. Mol. Res. 2(4):