Effect of transvaginal ultrasound on human chorionic villus cell apoptosis during pregnancy
Authors: X.L. Qu, H.T. Wang, J.L. Zou, L. Cheng, F. Wang, L.L. Ma and J. Li
With the advancement of ultrasonic technology in recent years, sonography has become a common medical diagnostic tool, as it has elevated output sonic intensity and elongated exposure time. This study investigates the effect of ultrasound on human chorionic villus cell apoptosis during early pregnancy. Transvaginal ultrasound was performed for a total of .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.26
- DOI:
- 10.4238/2015.December.28.26
Cytogenetic evaluation of tannery workers in the city of Teresina, northeastern Brazil
Authors: F.P.M. do Amaral, E.J.L.S. do Amaral, M.P.M. do Amaral, L.S. Lopes, S.I. Lemos, R.V.G. Santos, T.V.S. Chaves, M.E.A. de Moraes and M.O.A. de Moraes Filho
Assessments of chromosomal integrity and structure enable the prevention of diseases associated with the work environment, with the frequencies of chromosomal aberrations and micronuclei often being used as markers in biomonitoring. Owing to their routine manipulation of potentially toxic chemicals, tannery workers as a group merit a more thorough evaluat.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.29
- DOI:
- 10.4238/2015.December.28.29
An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Authors: R.R. Oliveira, A.J.C. Viana, A.C.E. Re�¡tegui and M.G.A. Vincentz
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.32
- DOI:
- 10.4238/2015.December.28.32
Y-STR INRA189 polymorphisms in Chinese yak breeds
Authors: Z.J. Ma, S.M. Chen, Y.G. Sun, Y.L. Xi, R.Z. Li, J.T. Xu and C.Z. Lei
To further explore Y-STR INRA189 polymorphisms in the yak, and to determine the genetic differences among yak breeds, genotyping analysis of INRA189 in 102 male yak individuals from three yak breeds in Qinghai Province of China was performed. Genotyping revealed the presence of four alleles, with sizes of 149, 155, 157, and 159 bp, respectively. Of these,.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.35
- DOI:
- 10.4238/2015.December.28.35
Downregulation of serum DKK-1 predicts poor prognosis in patients with papillary thyroid cancer
Authors: Y.P. Zhao, W. Wang, X.H. Wang, Y. Xu, Y. Wang, Z.F. Dong and J.J. Zhang
The Wnt inhibitor dickkopf-1 (DKK-1) has been shown to be closely correlated with tumor initiation and progression in various types of cancers. However, the serum level of DKK-1 in patients with papillary thyroid cancer (PTC) and its potential clinical significance is poorly understood. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the le.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.38
- DOI:
- 10.4238/2015.December.28.38
Effect of sinomenine on the expression of rheumatoid arthritis fibroblast-like synoviocytes MyD88 and TRAF6
Authors: H.C. Zhang, M.X. Liu, E.P. Wang, Z. Lin, G.F. Lv and X. Chen
The effect of sinomenine (SIN) on the toll-like receptor (TLR) signal transduction pathway as well as the expression of myeloid differentiation factor 88 (MyD88) and tumor necrosis factor (TNF) receptor-associated factor-6 (TRAF6) was investigated. SIN inhibition of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) proliferation and RA cartilag.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.41
- DOI:
- 10.4238/2015.December.28.41
miR-96 inhibits cardiac hypertrophy by targeting growth factor receptor-bound 2
Authors: Y. Xia, J. Sheng, G.Y. Liang, D.X. Liu, Q. Tang and A.P. Cheng
Increasing evidence has indicated that microRNAs are involved in the pathogenesis of cardiac hypertrophy. However, whether miR-96 is involved in heart diseases, particularly cardiac hypertrophy, remains unclear. In this study, we found that miR-96 is a negative regulator of cardiac hypertrophy. In primary cardiomyocytes, overexpression of miR-96 inhibited.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.2
- DOI:
- 10.4238/2015.December.29.2
Analysis of specific sequences in mutant rice generated by introduction of exogenous corn DNA
Authors: W.C. Li, S.D. Ji, C.Z. Tian, Z.K. Li, X.C. Wang, H.C. Zhang, Y.L. Sun, X.Z. Shi, T.X. Wang and J.Y. Li
Rice variation induced by the introduction of exogenous DNA has become an important method of improving rice varieties and creating new germplasms. In this study, we transferred maize genomic DNA fragments to the receptor of Nipponbare rice using a modified “pollen-tube pathway” method. Material from mutant rice B1 and B2 were acquired and 14 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.5
- DOI:
- 10.4238/2015.December.29.5
Transplantation of erythropoietin gene-transfected umbilical cord mesenchymal stem cells as a treatment for limb ischemia in rats
Authors: J.P. Li, D.W. Wang and Q.H. Song
The aim of this study was to investigate the mechanisms of erythropoietin (EPO)-transfected umbilical cord mesenchymal stem cells (UC-MSCs) in the treatment of rats with ischemic limb and provide a theoretical basis for optimization of UC-MSC transplantation. Sixty SD rats were randomly divided into four groups: ischemia control group, EPO treatment group.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.8
- DOI:
- 10.4238/2015.December.29.8
Rutin, quercetin, and free amino acid analysis in buckwheat (Fagopyrum) seeds from different locations
Authors: C.Z. Bai, M.L. Feng, X.L. Hao, Q.M. Zhong, L.G. Tong and Z.H. Wang
In this study, five common buckwheats and nine tartary buckwheats grown at different locations were analyzed for the contents of rutin, quercetin, and amino acids by high-performance liquid chromatography and spectrophotometry. The rutin content was higher than quercetin in buckwheat seeds. Rutin content was in the range from 0.05 (0.05 g per 100 g dry se.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.11
- DOI:
- 10.4238/2015.December.29.11