An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Authors: R.R. Oliveira, A.J.C. Viana, A.C.E. Re�¡tegui and M.G.A. Vincentz
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.32
- DOI:
- 10.4238/2015.December.28.32
Y-STR INRA189 polymorphisms in Chinese yak breeds
Authors: Z.J. Ma, S.M. Chen, Y.G. Sun, Y.L. Xi, R.Z. Li, J.T. Xu and C.Z. Lei
To further explore Y-STR INRA189 polymorphisms in the yak, and to determine the genetic differences among yak breeds, genotyping analysis of INRA189 in 102 male yak individuals from three yak breeds in Qinghai Province of China was performed. Genotyping revealed the presence of four alleles, with sizes of 149, 155, 157, and 159 bp, respectively. Of these,.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.35
- DOI:
- 10.4238/2015.December.28.35
Downregulation of serum DKK-1 predicts poor prognosis in patients with papillary thyroid cancer
Authors: Y.P. Zhao, W. Wang, X.H. Wang, Y. Xu, Y. Wang, Z.F. Dong and J.J. Zhang
The Wnt inhibitor dickkopf-1 (DKK-1) has been shown to be closely correlated with tumor initiation and progression in various types of cancers. However, the serum level of DKK-1 in patients with papillary thyroid cancer (PTC) and its potential clinical significance is poorly understood. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the le.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.38
- DOI:
- 10.4238/2015.December.28.38
Effect of sinomenine on the expression of rheumatoid arthritis fibroblast-like synoviocytes MyD88 and TRAF6
Authors: H.C. Zhang, M.X. Liu, E.P. Wang, Z. Lin, G.F. Lv and X. Chen
The effect of sinomenine (SIN) on the toll-like receptor (TLR) signal transduction pathway as well as the expression of myeloid differentiation factor 88 (MyD88) and tumor necrosis factor (TNF) receptor-associated factor-6 (TRAF6) was investigated. SIN inhibition of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) proliferation and RA cartilag.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.41
- DOI:
- 10.4238/2015.December.28.41
miR-96 inhibits cardiac hypertrophy by targeting growth factor receptor-bound 2
Authors: Y. Xia, J. Sheng, G.Y. Liang, D.X. Liu, Q. Tang and A.P. Cheng
Increasing evidence has indicated that microRNAs are involved in the pathogenesis of cardiac hypertrophy. However, whether miR-96 is involved in heart diseases, particularly cardiac hypertrophy, remains unclear. In this study, we found that miR-96 is a negative regulator of cardiac hypertrophy. In primary cardiomyocytes, overexpression of miR-96 inhibited.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.2
- DOI:
- 10.4238/2015.December.29.2
Analysis of specific sequences in mutant rice generated by introduction of exogenous corn DNA
Authors: W.C. Li, S.D. Ji, C.Z. Tian, Z.K. Li, X.C. Wang, H.C. Zhang, Y.L. Sun, X.Z. Shi, T.X. Wang and J.Y. Li
Rice variation induced by the introduction of exogenous DNA has become an important method of improving rice varieties and creating new germplasms. In this study, we transferred maize genomic DNA fragments to the receptor of Nipponbare rice using a modified “pollen-tube pathway” method. Material from mutant rice B1 and B2 were acquired and 14 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.5
- DOI:
- 10.4238/2015.December.29.5
Transplantation of erythropoietin gene-transfected umbilical cord mesenchymal stem cells as a treatment for limb ischemia in rats
Authors: J.P. Li, D.W. Wang and Q.H. Song
The aim of this study was to investigate the mechanisms of erythropoietin (EPO)-transfected umbilical cord mesenchymal stem cells (UC-MSCs) in the treatment of rats with ischemic limb and provide a theoretical basis for optimization of UC-MSC transplantation. Sixty SD rats were randomly divided into four groups: ischemia control group, EPO treatment group.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.8
- DOI:
- 10.4238/2015.December.29.8
Rutin, quercetin, and free amino acid analysis in buckwheat (Fagopyrum) seeds from different locations
Authors: C.Z. Bai, M.L. Feng, X.L. Hao, Q.M. Zhong, L.G. Tong and Z.H. Wang
In this study, five common buckwheats and nine tartary buckwheats grown at different locations were analyzed for the contents of rutin, quercetin, and amino acids by high-performance liquid chromatography and spectrophotometry. The rutin content was higher than quercetin in buckwheat seeds. Rutin content was in the range from 0.05 (0.05 g per 100 g dry se.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.11
- DOI:
- 10.4238/2015.December.29.11
Isolation, characterization, and multiplexing of novel microsatellite markers for the tropical scalloped spiny lobster (Panulirus homarus)
Authors: M. Delghandi, S. Goddard, D.R. Jerry, H.T. Dao, H. Afzal and S.S. Al-Jardani
Of the various spiny lobster species in the tropical and subtropical Indo-West Pacific region, the tropical scalloped spiny lobster (Panulirus homarus) supports one of the most commercially valuable fishery resources in many coastal African and Asian countries. The last decade has witnessed a serious decline in the wild populations of this species. Knowle.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.14
- DOI:
- 10.4238/2015.December.29.14
Glucagon-like peptide-1 receptor agonists inhibit hepatic stellate cell activation by blocking the p38 MAPK signaling pathway
Authors: L.K. Wu, Y.C. Liu, L.L. Shi and K.D. Lu
We investigated the effects of glucagon-like peptide-1 receptor (GLP-1R) agonists on p38 mitogen-activated protein kinase (MAPK) signaling during inhibition of hepatic stellate cell (HSC) activity. Human HSCs were cultured and morphologically identified. HSC samples were collected and randomly divided into three groups (N = 20 samples per group): a contro.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.17
- DOI:
- 10.4238/2015.December.29.17