Cloning and characterization of DELLA genes in Artemisia annua
Authors: Q. Shen, J. Cui, X.Q. Fu, T.X. Yan and K.X. Tang
Gibberellins (GA) are some of the most important phytohormones involved in plant development. DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed that AaDELLA1 and AaDELLA2 were located in the same cluster,.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.10
- DOI:
- 10.4238/2015.August.21.10
Association of TCF7L2 gene polymorphisms with susceptibility to type 2 diabetes mellitus in a Chinese Hui population
Authors: Y. Yang, J.R. Xu, Y.J. Wang and X.M. Li
Diabetes is one of costly chronic diseases. Previous studies across several ethnicities have shown that polymorphisms in the transcription factor 7-like 2 (TCF7L2) gene were strongly associated with susceptibility to type 2 diabetes (T2DM). In the present study, the association between the TCF7L2 gene and the susceptibility to T2DM in a Chinese Hui popula.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.13
- DOI:
- 10.4238/2015.August.21.13
Antiviral effect of hepatitis B virus S/C gene loci antisense locked nucleic acid on transgenic mice in vivo
Authors: Y.B. Deng, H.J. Qin, Y.H. Luo, Z.R. Liang and J.J. Zou
We investigated the effects of hepatitis B virus (HBV) S/C double gene loci antisense locked nucleic acid on replication and expression of HBV in hepatitis transgenic mice. HBV mice (N = 30) were randomly divided into five groups of six mice: 5% glucose solution control, empty liposome control, single-target S, single-target C, and dual-target SC groups. .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.16
- DOI:
- 10.4238/2015.August.21.16
Identification and analysis of the TIFY gene family in Gossypium raimondii
Authors: D.H. He, Z.P. Lei, B.S. Tang, H.Y. Xing, J.X. Zhao and Y.L. Jing
The highly conserved TIFY domain is included in the TIFY protein family of transcription factors, which is important in plant development. Here, 28 TIFY family genes were identified in the Gossypium raimondii genome and classified into JAZ (15 genes), ZML (8), PPD (3), and TIFY (2). The normal (TIF[F/Y]XG) motif was dominant in the TIFY family, excluding .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.19
- DOI:
- 10.4238/2015.August.21.19
Differential expression of TLP, ERF1, and R2R3MYB in annual Medicago species under salinity conditions
Authors: F. Gharaghani, F. Rafiei, N. Mirakhorli and E. Ebrahimie
The present study was conducted to evaluate the responses of three annual Medicago species (M. truncatula, M. laciniata, and M. polymorpha) to salinity. We analyzed publicly available microarray data in NCBI pertaining to salinity-response genes in M. truncatula. Our data search identified Tubby C2 (TLP) and ethylene responsive transcription factor 1 (ERF.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.22
- DOI:
- 10.4238/2015.August.21.22
Effect of DNase treatment on RNA extraction from preimplantation murine embryos
Authors: J. Norhazlin, M.N.K. Nor-Ashikin, B.P. Hoh, S.H. Sheikh Abdul Kadir, S. Norita, M. Mohd-Fazirul, W.J. Wan-Hafizah, D. Razif, M.H. Rajikin and B. Abdullah
The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.1
- DOI:
- 10.4238/2015.August.28.1
In vitro expansion and differentiation of rat pancreatic duct-derived stem cells into insulin secreting cells using a dynamic three-dimensional cell culture system
Authors: X.C. Chen, H. Liu, H. Li, Y. Cheng, L. Yang and Y.F. Liu
In this study, a dynamic three-dimensional cell culture technology was used to expand and differentiate rat pancreatic duct-derived stem cells (PDSCs) into islet-like cell clusters that can secrete insulin. PDSCs were isolated from rat pancreatic tissues by in situ collagenase digestion and density gradient centrifugation. Usi.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028808
- DOI:
- 10.4238/gmr.15028808
Transcriptomic differences of genes in the avian target of rapamycin (avTOR) pathway in a divergent line of meat-type chickens selected for feed efficiency
Authors: J. Lee and S.E. Aggrey
Avian target of rapamycin (avTOR) is a highly conserved serine-threonine kinase that serves as an intracellular energy and nutrient sensor and regulates cell division, growth, and apoptosis. The role of avTOR in mediating feed intake and growth in poultry is unknown. We studied avTOR signaling activities in duodenum and liver .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027120
- DOI:
- 10.4238/gmr.15027120
Investigation of genes in chronic and acute morphine-treated mice using microarray datasets
Authors: L. Ding, J.L. Zhang, S.H. Yu and L.F. Sheng
Morphine is a psychoactive medication that is used as a standard analgesic treatment to relieve pain in clinics. Many patients rely on chronic or acute treatment of morphine to treat pain. However, morphine is a narcotic and has a reverse effect when inappropriately used. Therefore, it is necessary to study chronic and acute morphine treatment to improve .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.3
- DOI:
- 10.4238/2015.August.28.3
Protein-protein interaction between ezrin and p65 in human breast cancer cells
Authors: R. Tang, F.X. Li, W.F. Shao, Q.S. Wen, X.R. Yu and J.B. Xiong
Our study aimed to investigate the co-localization and protein-protein interactions between ezrin and p65 in human breast cancer cells. Liquid chromatography-mass spectrometry (LCMS) was used to uncover novel protein interactions with ezrin in MDA-MB-231 cells. Endogenous co-immunoprecipitation was used to validate protein-pro.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028334
- DOI:
- 10.4238/gmr.15028334