Identifying significant pathways of hepatitis B virus-related hepatocellular carcinoma based on crosstalk and network pathways
Authors: L.M. Wen, W.Z. Wu and X.C. Peng
This study aims to identify significant pathways in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) based on the pathway network strategy. We proposed a pathway network where a protein-protein interaction (PPI) network was integrated with the crosstalk of pathways. Pathway data were first obtained from backgroun.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028343
- DOI:
- 10.4238/gmr.15028343
Molecular cloning and expression analysis of a pearl oyster (Pinctada martensii) heat shock protein 90 (HSP90)
Authors: H.Y. Liang, Z.X. Wang, Q.N. Lei, R.L. Huang, Y.W. Deng, Q.H. Wang, Y. Jiao and X.D. Du
Heat shock protein 90 (HSP90) is an important molecular chaperone required for proper folding of cellular proteins, and thus, it plays an essential role in protecting cells from damage during stress. In this study, an HSP90 cDNA designated PmHSP90 was cloned from the mantle tissue of the pearl oyster Pinctada martensii using reverse transcription polymera.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.27
- DOI:
- 10.4238/2015.December.28.27
Selecting the optimum conditions for two-dimensional difference gel electrophoresis of proteins expressed in Populus euphratica
Authors: J.Q. Hao, N. Yue and C.X. Zheng
We selected the optimum conditions for two-dimensional difference gel electrophoresis (2D-DIGE) of proteins expressed in the heteromorphic leaves of Populus euphratica Oliv. by adjusting the isoelectric focusing, the loading quantity, the concentration of the electrophoretic gel, and other parameters. The results of our study .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028360
- DOI:
- 10.4238/gmr.15028360
Data integration for identification of important transcription factors of STAT6-mediated cell fate decisions
Authors: M. Jargosch, S. Kr�¶ger, E. Gralinska, U. Klotz, Z. Fang, W. Chen, U. Leser, J. Selbig, D. Groth and R. Baumgrass
Data integration has become a useful strategy for uncovering new insights into complex biological networks. We studied whether this approach can help to delineate the signal transducer and activator of transcription 6 (STAT6)-mediated transcriptional network driving T helper (Th) 2 cell fate decisions. To this end, we performe.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028493
- DOI:
- 10.4238/gmr.15028493
miRNA-146a induces vascular smooth muscle cell apoptosis in a rat model of coronary heart disease via NF-�ºB pathway
Authors: Z.W. Wu, Y.F. Liu, S. Wang and B. Li
The aim of this study was to investigate the role of miRNA-146a in modulating the function of vascular smooth muscle cells in a rat model of coronary heart disease. Vascular smooth muscle cells were isolated and cultured from the rat coronary heart disease model and normal rats (controls). miRNA-146a levels were measured in vascular smooth muscle cells ob.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.19
- DOI:
- 10.4238/2015.December.28.19
Effect of uric acid on mitochondrial function and oxidative stress in hepatocytes
Authors: Y. Yang, Y. Zhou, S. Cheng, J.L. Sun, H. Yao and L. Ma
Here, we investigated the effect of uric acid (UA) on hepatocyte mitochondria. Hepatocytes cultured in vitro were treated with varying concentrations of UA. The change in apoptotic activity was detected by flow cytometry. The DNA damage index 8-hydroxy-deoxy-guanosine (8-OHdG) and mitochondrial function indices succinate dehyd.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028644
- DOI:
- 10.4238/gmr.15028644
Male carriers of balanced reciprocal translocations in Northeast China: sperm count, reproductive performance, and genetic counseling
Authors: H.G. Zhang, R.X. Wang, L.L. Li, W.T. Sun, H.Y. Zhang and R.Z. Liu
Balanced chromosomal translocations in men can cause failure of spermatogenesis owing to meiotic impairment. Male carriers may exhibit normozoospermia, although clinical manifestations can include oligozoospermia or azoospermia, oligozoospermia or normozoospermia. Here, we reported the characteristics of balanced reciprocal translocations in men from nort.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.28
- DOI:
- 10.4238/2015.December.28.28
Molecular tracing of white muscardine in Asian corn borer using inter-simple sequence repeat (ISSR) analysis
Authors: B.J. Hu, L.N. Xu, Z.Y. Zhou, F. Hu, F.G. Luan, X. Chen and Z.Z. Li
Beauveria bassiana is a soil fungus that parasitizes arthropod species, and is used to control the Asian corn borer in Northeast China. In this study, B. bassiana was investigated in Xiaoxian County and Baicheng City, and the results were compared with those of Gongzhuling City, where the fungus was not applied. Using the inter-simple sequence repeat (ISS.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.21
- DOI:
- 10.4238/2015.December.28.21
Correlation of increased MALAT1 expression with pathological features and prognosis in cancer patients: a meta-analysis
Authors: X.S. Shi, J. Li, R.H. Yang, G.R. Zhao, H.P. Zhou, W.X. Zeng and M. Zhou
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been identified as a potential cancer biomarker, yet the mechanism by which it influences the development of cancer remains unknown. In this study, we aimed to correlate MALAT1 expression with pathological features and prognosis in cancer patients. Several databases were searched using co.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.30
- DOI:
- 10.4238/2015.December.28.30
Transcriptomic analysis of Camellia ptilophylla and identification of genes associated with flavonoid and caffeine biosynthesis
Authors: M.M. Li, J.Y. Xue, Y.L. Wen, H.S. Guo, X.Q. Sun, Y.M. Zhang and Y.Y. Hang
Camellia ptilophylla, or cocoa tea, is naturally decaffeinated and its predominant catechins and purine alkaloids are trans-catechins and theobromine Regular tea [Camellia sinensis (L.) O. Ktze.] is evolutionarily close to cocoa tea and produces cis-catechins and caffeine. Here, the transcriptome of C. ptilophylla was sequenced using the 101-bp paired-end.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.22
- DOI:
- 10.4238/2015.December.28.22