Effect of Bifidobacterium on the mRNA expression levels of TRAF6, GSK-3�², and microRNA-146a in LPS-stimulated rat intestinal epithelial cells
Authors: W. Zhou, Y. Yuan, J. Li, W.M. Yuan, L.G. Huang and S.W. Zheng
We investigated the effect of inactivated Bifidobacterium on the mRNA expression of TRAF6, GSK-3β, and microRNA-146a in lipopolysaccharide (LPS)-stimulated rat small intestinal epithelial cells (IEC-6s). IEC-6s were randomly divided into an LPS group, a culture supernatant group, and an inactivated bacteria group. After stimulation with LPS for 5 h, .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.11
- DOI:
- 10.4238/2015.August.21.11
Analysis of the relationship between peripheral blood T lymphocyte subsets and HCV RNA levels in patients with chronic hepatitis C.
Authors: Y. Huang, M.J. Zheng and Y.H. Xu
We investigated the relationship between peripheral blood T lymphocyte subsets and hepatitis C virus (HCV) RNA levels in patients with hepatitis C. Samples from 69 chronic hepatitis C (CHC) patients and 20 healthy controls were analyzed using quantitative polymerase chain reaction (PCR) to detect HCV RNA and flow cytometry to determine the expression leve.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.12
- DOI:
- 10.4238/2015.August.21.12
Heterosis and correlation in interspecific and intraspecific hybrids of cotton
Authors: S. Munir, S.B. Hussain, H. Manzoor, M.K. Quereshi, M. Zubair,W. Nouman, A.N. Shehzad, S. Rasul and S.A. Manzoor
Interspecific and intraspecific hybrids show varying degrees of heterosis for yield and yield components. Yield-component traits have complex genetic relationships with each other. To determine the relationship of yield-component traits and fiber traits with seed cotton yield, six lines (Bt. CIM-599, CIM-573, MNH-786, CIM-554,.. Read More»
- Genet. Mol. Res. 15(2):
Establishment of a blue light damage model of human retinal pigment epithelial cells in vitro
Authors: G. Su, S.J. Cai, X. Gong, L.L. Wang, H.H. Li and L.M. Wang
To establish a blue-light damage model of human retinal pigment epithelium (RPE). Fourth-generation human RPE cells were randomly divided into two groups. In group A, cells were exposed to blue light (2000 ± 500 lux) for 0 (control), 3, 6, 9, and 12 h, and cell culture was stopped after 12 h. In group B, cells were expo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028092
- DOI:
- 10.4238/gmr.15028092
HPRTYale proposed as a pathogenic variant for Lesch-Nyhan syndrome: a case report
Authors: E. Stur, R.S. Reis, L.P. Agostini, A.M.A. Silva-Conforti and I.D. Louro
Table of Contents | Genet. Mol. Res. 2016 (2) Research Article Effect of uric acid on mitochondrial function and oxidative stress in hepatocytes Authors: Y. Yang Y. Zhou S. Cheng J.L. Sun H. Yao L. Ma Here, we investigated the effect of uric acid (UA) on hepatocyte mitochondria. Hepatocytes cultured i.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028251
- DOI:
- 10.4238/gmr.15028251
Genetic variation in wild populations of the tuber crop Amorphophallus konjac (Araceae) in central China as revealed by AFLP markers
Authors: C. Pan, A.W. Gichira and J.M. Chen
Amorphophallus konjac is an economically important crop. In order to provide baseline information for sustainable development and conservation of the wild plant resources of A. konjac, we studied the genetic diversity and population structure of this species using amplified fragment length polymorphism (AFLP) molecular markers. We sampled 139 individuals .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.24
- DOI:
- 10.4238/2015.December.28.24
High polymorphism at microsatellite loci in the Chinese donkey
Authors: R.F. Zhang, W.M. Xie, T. Zhang and C.Z. Lei
To reveal the genetic diversity and phylogenetic relationships between Chinese donkey breeds, 415 individuals representing ten breeds were investigated using ten microsatellite markers. The observed number of alleles, mean effective number of alleles (NE), mean expected heterozygosity (HE), and polymorphic information content .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028291
- DOI:
- 10.4238/gmr.15028291
Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization
Authors: Z.B. Yu, C.K. Mu, W.W. Song, R.H. Li, Y.E. Chen and C.L. Wang
The swimming crab, Portunus trituberculatus, is an important marine animal and is widely cultured in China. In the present study, suppression subtractive hybridization was applied to identify the differentially expressed genes in the ovaries of mature and immature P. trituberculatus. One hundred and seventy six expressed sequence tag (ESTs) were identifie.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.16
- DOI:
- 10.4238/2015.December.28.16
Effect of SNX-2112 on proliferation of esophageal cancer cells via regulation of excision repair cross-complementing 1, epidermal growth factor receptor, and p53 expression
Authors: C.Y. Li, Y.J. Ren and Y.D. Li
SNX-2112 is a potential molecular targeted therapeutic drug against esophageal cancer (EC). However, its exact mechanism of action remains to be explained. The aim of this study was to investigate the effect of SNX-2112 on excision repair cross- complementing 1 (ERCC1), epidermal growth factor receptor (EGFR), and p53, to eluc.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028318
- DOI:
- 10.4238/gmr.15028318
Analysis and application of ATP7B gene mutations in 35 patients with hepatolenticular degeneration
Authors: Y.N. Zong and X.D. Kong
We investigated the genetic mutations involved in Wilson’s disease to improve prenatal genetic diagnosis and presymptomatic diagnosis. The polymerase chain reaction (PCR) was used to amplify the exons and exon-intron boundaries of the ATP7B gene in 35 Wilson’s disease pedigrees. The PCR products were further analyzed by Sanger sequencing. Pren.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.25
- DOI:
- 10.4238/2015.December.28.25