Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue
Authors: L. Liu, C.L. Wang, W.Y. Peng, J. Yang, M.Q. Lan, B. Zhang, J.B. Li, Y.Y. Zhu and C.Y. Li
Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimeth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.1
- DOI:
- 10.4238/2015.December.28.1
Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids
Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu
Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»
- Genet. Mol. Res. 15(2):
Influence of sirolimus-induced TGF-�² secretion on mouse Treg cell proliferation
Authors: J.N. Li, J.X. Li, H.L. Huang, X.L. Zhu, H. Ding, C.F. Huang, J. Lin, J.G. Huang, Z.C. Wu, M. Ashraf, Y.G. Wang, X.K. Li, S.Y. Zheng, J.M. Chen, H.M. Guo, J. Zhuang and P. Zhu
We examined the effects of co-culturing CD4+ CD25+ Treg cells with sirolimus or cyclosporin A on Treg cell proliferation and differentiation and on transforming growth factor-β (TGF-β) and Foxp3 expression. CD4+ CD25+ Treg cells were harvested from mononuclear cells of spleens of C57BL/6 mice using immunomagnetic beads and divided into control, .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.4
- DOI:
- 10.4238/2015.December.28.4
Mitochondrial tRNA mutations may be infrequent in hepatocellular carcinoma patients
Authors: G. Li, Y.X. Duan, X.B. Zhang and F. Wu
Mitochondrial DNA mutations have been shown to play important roles in the pathogenesis of hepatocellular carcinoma (HCC). In particular, genes encoding mitochondrial tRNA (mt-tRNA) are hotspots for pathogenic mutations associated with HCC. Recently, an increasing number of studies have reported the involvement of such mutatio.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027665
- DOI:
- 10.4238/gmr.15027665
Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP
Authors: Y. Zhang, X. Yang, F.Y. Zhou, A.F. Zhang, X.F. Zhu, Y. Chen, M.G. Zhou and T.C. Gao
The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.6
- DOI:
- 10.4238/2015.December.28.6
Effect of MSTN propeptide protein on the growth and development of Altay lamb muscle
Authors: W. Du, Y. Zhang, J.Z. Yang, H.B. Li, J. Xia, N. Li, J.S. Zhang, X.M. Yan and Z.Y. Zhou
Prokaryotic expression technology was used to express maltose-binding protein binding myostatin (MSTN) propeptide fusion protein. Six disease-free Altay lambs were used in this study. The right leg gastrocnemii were injected with MSTN recombinant propeptide protein. The left leg gastrocnemii (the control group) were injected w.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027778
- DOI:
- 10.4238/gmr.15027778
Evaluation of novel assays for the detection of human papilloma virus in self-collected samples for cervical cancer screening
Authors: Q. Chen, H. Du, R. Zhang, J.H. Zhao, Q.C. Hu, C. Wang, G.X. Wang, J.L. Tang and R.F. Wu
The aim of this study was to evaluate the performance of three new high-risk human papillomavirus (HPV) assays for primary cervical cancer screening, by using self-collected samples, and to identify an HPV assay that could overcome the major obstacles faced during large-scale population-based screening. Two hundred and ten wom.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027896
- DOI:
- 10.4238/gmr.15027896
Association of LT�²R gene polymorphisms with prostate volume in benign prostatic hyperplasia in the Korean population
Authors: S.H. Lee, S.K. Kim, K.H. Yoo, J.H. Chung and S.G. Chang
The lymphotoxin-β receptor (LTβR) gene is involved in autoimmune disease and inflammatory disorder development, but the relationship between LTβR and benign prostatic hyperplasia (BPH) is unclear. In total, 222 with BPH were examined for 3 single nucleotide polymorphisms [rs3759333 (-1387C/T), rs3759334 (-1326A/G), and rs2364480 (Ala172Ala).. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.9
- DOI:
- 10.4238/2015.December.28.9
Sex- and age-dependent expression of Pax7, Myf 5, MyoG, and Myostatin in yak skeletal muscles
Authors: G. Wu, J. Zhang, L. Wang, S. Xu, J. Zhou, A. Xiang and C. Yang
The aim of this study was to investigate the myogenic factor mRNA expression pattern of Pax7, Myf5, MyoG, and Myostatin mRNA at different ages, sexes, and muscle tissues of Datong yaks. The expression patterns in semimembranosus (SM), quadriceps femoris (QF), and triceps muscle of arm (TM) were detected by quantitative real-ti.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028020
- DOI:
- 10.4238/gmr.15028020
In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes
Authors: W. Ji, G.R. Li , Y.X. Luo, X.H. Ma, M. Wang and R. Ren
Crossing different ploidy grapes is an effective way to obtain new seedless cultivars. Although embryo rescue has been extensively applied in breeding seedless and triploid grapes, only a few improved cultivars have been developed. Based on preliminary studies, we set five crosses between tetraploid and diploid grape varieties to obtain new hybrid triploi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.10
- DOI:
- 10.4238/2015.December.28.10