Can Spirulina maxima reduce the mutagenic potential of sibutramine?
Authors: R.P. Araldi, N.P. Santos, T.B. Mendes, L.B. Carvalho, E.T. Ito, P.L. de-S?¡-J?ºnior and EB Souza
The worldwide obesity pandemic requires the use of anti-obesity drugs. Sibutramine is an anti-obesity drug that has been used worldwide but is indiscriminately consumed in Brazil. Several studies have demonstrated that sibutramine promotes weight loss and weight maintenance, but several side effects have been associated with its systematic consumption. Fo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.33
- DOI:
- 10.4238/2015.December.23.33
Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)
Authors: Z.Z. Xiao, W.W. Chen, W. Bao, R. Wang and Y.Y. Li
Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populatio.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028322
- DOI:
- 10.4238/gmr.15028322
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
Authors: M.S.P. De Azevedo, C. Santos Rocha, V.B. Pereira, A.F. De Oliveira Junior, C.S. De Sousa, V. Azevedo, J.G. LeBlanc, J.M. Chatel and A. Miyoshi
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with hig.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.36
- DOI:
- 10.4238/2015.December.23.36
Screening of basidiomycetes in submerged cultivation based on antioxidant activity
Authors: S.H. Umeo, G.P.N. Souza, P.M. Rapachi, D.M. Garcia, L.D. Paccola-Meirelles, J.S. Valle, N.B. Colauto and G.A. Linde
Submerged cultivation of medicinal basidiomycetes is a reproducible and efficient method of producing mycelia and metabolites. The antioxidant activity indicates its medicinal properties and is an important tool for basidiomycete screening. In this study, we analyzed the production of mycelial biomass and exopolysaccharides and the antioxidant activity of.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.25
- DOI:
- 10.4238/2015.August.19.25
Effect of acute/subchronic samarium exposure on the concentration, motility, and morphology of sperm in male mice
Authors: D.Y. Zhang, X.Y. Shen, X.L. Xu, Q. Ruan, SS. Hu, Y.Y. Chen and Z.M. Wang
Male ICR mice were orally administered samarium nitrate [Sm(NO3)3] to investigate its effects on sperm concentration and sperm quality. After acute exposure to ≥2880.00 mg/kg Sm(NO3)3 via intragastric gavage, sperm motility and acrosome integrity were decreased, and the sperm malformation percentage was increased (P 3)3 adm.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028807
- DOI:
- 10.4238/gmr.15028807
Operational taxonomic units (OTUs) of endophytic bacteria isolated from banana cultivars in the Amazon
Authors: ?. Souza, V.B. Nogueira, J.C. Cruz, N.R. Sousa, A.R.L. Proc?³pio and G.F. Silva
Endophytic microorganisms colonize plants, inhibit the growth of pathogens (by competing for nutrients and/or space), or produce antagonistic substances. Fifty-five endophytic bacteria were isolated from the leaf tissue of the FHIA 18 banana cultivar. Genetic diversity analyses were performed using the enterobacterial repetitive intergenic consensus seque.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.28
- DOI:
- 10.4238/2015.August.19.28
HVSI polymorphism indicates multiple origins of mtDNA in the Hazarewal population of Northern Pakistan
Authors: N. Akbar, H. Ahmad, M.S. Nadeem, B.E. Hemphill, K. Muhammad, W. Ahmad and M. Ilyas
Mitochondrial DNA (mtDNA) is an important tool used to explore ethnogenetics and the evolutionary history of human populations. In this study, hypervariable segment I (HVSI) from mtDNA was analyzed to establish the genetic lineage of the Hazarewal populations residing in the Mansehra and Abbottabad districts of Northern Pakist.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027167
- DOI:
- 10.4238/gmr.15027167
Association between ARNTL (BMAL1) rs2278749 polymorphism T >C and susceptibility to Alzheimer disease in a Chinese population
Authors: Q. Chen, X.D. Peng, C.Q. Huang, X.Y. Hu and X.M. Zhang
In the present study, we examined whether the ARNTL (BMAL1) rs2278749 T/C polymorphism was associated with the susceptibility to Alzheimer disease (AD). This case-control study examined the genotypes of apolipoprotein E (APOE e4) and BMAL1 rs2278749 T/C using restriction fragment length polymorphism and the TaqMan assay, respectively. A total of 296 unrel.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.39
- DOI:
- 10.4238/2015.December.23.39
Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue
Authors: L. Liu, C.L. Wang, W.Y. Peng, J. Yang, M.Q. Lan, B. Zhang, J.B. Li, Y.Y. Zhu and C.Y. Li
Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimeth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.1
- DOI:
- 10.4238/2015.December.28.1
Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids
Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu
Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»
- Genet. Mol. Res. 15(2):