Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease
Authors: C.H. Shan, F.X. Tang, W. Chen and W.R. Ma
Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.10
- DOI:
- 10.4238/2015.December.23.10
Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil
Authors: R.R. Simplicio, A.M. Waldschmidt, M.B. Amorim, B.S. Almeida and D.G. Pereira
Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.11
- DOI:
- 10.4238/2015.December.23.11
Expression of GFP can influence aging and climbing ability in Drosophila
Authors: R.M.S. Mawhinney and B.E. Staveley
Green fluorescent protein (GFP) is widely used as a reporter transgene in a variety of organisms. Some of the advantages of using GFP include non-invasive visualization of biological events and/or tissues in live specimens and its benign nature. When GFP is expressed throughout the organism, in neurons and eyes, lifespan and c.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1023
- DOI:
- 10.4238/vol10-1gmr1023
An outbreak of Candida parapsilosis fungemia among preterm infants
Authors: W. Guo, H.F. Gu, H.G. Zhang, S.B. Chen, J.Q. Wang, S.X. Geng, L. Li, P. Liu, X. Liu, Y.R. Ji, S.W. Li and L. Yang
In this article, we describe the first outbreak of Candida parapsilosis fungemia in our hospital. We examined a cluster of four nosocomial cases of C. parapsilosis fungemia that occurred in the neonatal intensive care unit (NICU) of the Affiliated Xingtai People’s Hospital of Hebei Medical University over a two-week period. We ascertained patient pa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.13
- DOI:
- 10.4238/2015.December.23.13
Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data
Authors: W.Q. Song, W.Q. Gu, Y.B. Qian, X. Ma, Y.J. Mao and W.J. Liu
The aim of this study was to identify long non-coding RNA (lncRNA) associated with osteogenic differentiation from mesenchymal stem cells (MSCs) using high-throughput RNA sequencing (RNA-Seq) data. RNA-Seq dataset was obtained from the European Bioinformatics Institute (accession No. PRJEB4496), including two replicates each for immortalized mesenchymal s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.14
- DOI:
- 10.4238/2015.December.23.14
Phylogenetic relationships of Malayan gaur with other species of the genus Bos based on cytochrome b gene DNA sequences
Authors: M.K.A. Rosli, S.S. Zakaria, S.M.F. Syed-Shabthar, Z.Z. Zainal, M.N. Shukor, M.C. Mahani, O. Abas-Mazni and B.M. Md-Zain
The Malayan gaur (Bos gaurus hubbacki) is one of the three subspecies of gaurs that can be found in Malaysia. We examined the phylogenetic relationships of this subspecies with other species of the genus Bos (B. javanicus, B. indicus, B. taurus, and B. grunniens). The sequence of a key gene, cytochrome b, was compared among 20.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1002
- DOI:
- 10.4238/vol10-1gmr1002
Effects of CD3McAb and rhIL-2 activated bone marrow on the killing and purging of leukemia cells
Authors: X.C. Wei, D.D. Yang, X.R. Han, Y.A. Zhao, Y.C. Li, L.J. Zhang and J.J. Wang
We investigated the roles of CD3McAb and rhIL-2 activated bone marrow in the killing and purging of leukemia cells. Cytotoxicity of activated bone marrow was detected with MTT assay. CFU-GM level in activated bone marrow and the destruction of leukemia cells were measured using the semi-solid cell culture. Immune activation markers in activated bone marro.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.16
- DOI:
- 10.4238/2015.December.23.16
Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus
Authors: J. Tavares, J.P. Bravo, F. Gimenes, Q.A.L. Neto, A. Fiorini and M.A. Fernandez
Previous reports demonstrated that actin is necessary for nucleocapsid transport and viral gene expression during nucleopolyhedrovirus infection of Bombyx mori. The first intron of B. mori A3 actin contains a cryptic promoter that drives expression of a rare isoform. We detected differences in the size and nucleotide compositi.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr978
- DOI:
- 10.4238/vol10-1gmr978
Study of the relationship between the expression of nerve growth factor and aneurysm formation and prognosis
Authors: C.D. Wang,D.K. Wang,P.C. Cao,Z.W. Wang,Z. Wang and Y.T. Wang
We sought to investigate the effect of nerve growth factor (NGF) expression on the formation and prognosis of cerebral aneurysms. Forty-eight cases were selected following a diagnosis of cerebral aneurysm using computed tomography angiography and surgical confirmation. Thirty-four cases of healthy deaths were also chosen. The tissue was tested for NGF exp.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.28.8
- DOI:
- http://dx.doi.org/10.4238/2015.April.28.8
RsaI polymorphism of the ER�² gene in women with endometriosis
Authors: R.C.P.C. Silva, I.R. Costa, B.M Bordin, C.T.X. Silva, S.R. Souza, C.L.R. J�ºnior, A.B. Frare and K.K.V.O. Moura
We examined the frequency of RsaI polymorphism of the ERβ gene in 54 patients diagnosed with endometriosis and 46 controls. Peripheral blood was collected from women undergoing laparoscopy with a confirmed diagnosis of endometriosis. Polymorphisms of the ERβ gene and p53 were assessed by PCR and analyzed on 2% agaros.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr940
- DOI:
- 10.4238/vol10-1gmr940