A minimal DNA cassette as a vector for genetic transformation of soybean (Glycine max)
Authors: G.R. Vianna, F.J.L. Arag�£o and E.L. Rech
Currently, the market demands products committed to protecting human health and the environment, known as clean products. We developed a protocol using DNA fragments containing only the gene sequence of interest, to replace the circular vectors containing genes for antibiotic resistance and other undesirable sequences, for obt.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1058
- DOI:
- 10.4238/vol10-1gmr1058
Genetic diversity and differentiation in Prunus species (Rosaceae) using chloroplast and mitochondrial DNA CAPS markers
Authors: S. Ben Mustapha,H. Ben Tamarzizt,G. Baraket,D. Abdallah and A. Salhi Hannachi
Chloroplast (cpDNA) and mitochondrial DNA (mtDNA) were analyzed to establish genetic relationships among Tunisian plum cultivars using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Two mtDNA regions (nad 1 b/c and nad 4 1/2) and a cpDNA region (trnL-trnF) were amplified and digested using restriction enzymes... Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.33
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.33
Muscle pain, fever, cough, and progressive dyspnea in a woman with eosinophilic pneumonia
Authors: Z. Xu,Y. Fan,G.S. Wang and G.M. Wu
The diagnosis of eosinophilic pneumonia (EP) is rare and challenging. This condition is frequently misdiagnosed as pulmonary tuberculosis, lymphoma, schistosomiasis, Wegener’s granuloma, severe acute respiratory syndrome, or severe community-acquired pneumonia. Herein, we report a case in which computed tomography (CT)-guided percutaneous lung biops.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.28.1
- DOI:
- http://dx.doi.org/10.4238/2015.April.28.1
Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms
Authors: T.B.A. Pessoa, S.S. de Souza, A.F. Cerqueira, R.P. Rezende, C.P. Pirovani and J.C.T. Dias
Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collec.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.28.2
- DOI:
- 10.4238/2013.June.28.2
Phylogenetic relationships of leaf monkeys (Presbytis; Colobinae) based on cytochrome b and 12S rRNA genes
Authors: V.F. Vun, M.C. Mahani, M. Lakim, A. Ampeng and B.M. Md-Zain
Little is known about the classification and phylogenetic relationships of the leaf monkeys (Presbytis). We analyzed mitochondrial DNA sequences of cytochrome b (Cyt b) and 12S rRNA to determine the phylogenetic relationships of the genus Presbytis. Gene fragments of 388 and 371 bp of Cyt b and 12S rRNA, respectively, were seq.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1048
- DOI:
- 10.4238/vol10-1gmr1048
Biological and molecular characterization of silkworm strains from the Brazilian germplasm bank of Bombyx mori
Authors: N.C. Pereira, R.E.F. Munhoz, T.S. Bignotto, R. Bespalhuk, L.B. Garay, C.R.N. Saez, V.A. Fassina, A. Nembri and M.A. Fernandez
Brazil has only one public genetic pool of Bombyx mori strains, which was established in 2005 at Universidade Estadual de Maringá, Maringá, Paraná State. This genetic bank has been maintained, and the strains have been characterized using genetic and morphological tools. The quantitative and qualitative traits, directly or indirectly .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.28.1
- DOI:
- 10.4238/2013.June.28.1
Lack of association of single nucleotide polymorphisms of the bovine Flt-1 gene with growth traits in Chinese cattle breeds
Authors: Y.H. Pang, C.Z. Lei, C.L. Zhang, X.Y. Lan, S. Shao, X.M. Gao, J.Q. Wang and H. Chen
We analyzed 20 exons, with their intron-exon boundaries, of the bovine Flt-1 gene, using a strategy combining PCR amplification and single-strand conformational polymorphism analysis (PCR-SSCP), followed by nucleotide sequence analysis, in 675 cattle. We then looked for associations between polymorphisms and growth traits. Twe.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1041
- DOI:
- 10.4238/vol10-1gmr1041
Experimental optimization of the number of blocks by means of algorithms parameterized by confidence interval in popcorn breeding
Authors: T.O.M. Paula, C.D. Marinho, A.T. Amaral J�ºnior, L.A. Peternelli and L.S.A. Gon�§alves
The objective of this study was to determine the optimal number of repetitions to be used in competition trials of popcorn traits related to production and quality, including grain yield and expansion capacity. The experiments were conducted in 3 environments representative of the north and northwest regions of the State of Rio de Janeiro with 10 Brazilia.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.27.2
- DOI:
- 10.4238/2013.June.27.2
Effect of lipoic acid combined with paclitaxel on breast cancer cells
Authors: B.J. Li, X.Y. Hao, G.H. Ren and Y. Gong
Breast cancer is the most common gynecologic tumor globally that threatens women’s health. Lipoic acid is a type of antioxidant that can alleviate oxidative stress damage. Studies showed that lipoic acid could inhibit the proliferation of tumor cells in cervical cancer and colon cancer. This paper intends to explore the combined effect of lipoic aci.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.18
- DOI:
- 10.4238/2015.December.22.18
Chromosome stickiness impairs meiosis and influences reproductive success in Panicum maximum (Poaceae) hybrid plants
Authors: C. Pessim,M.S. Pagliarini,N. Silva and L. Jank
Chromosome stickiness has been studied in several species of higher plants and is characterized by sticky clumps of chromatin resulting in sterility. Chromosome stickiness was recorded in Panicum maximum hybrid plants that were cultivated in the field. In the meiocytes affected, chromosomes clumped into amorphous masses that did not orient themselves on t.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.28.2
- DOI:
- http://dx.doi.org/10.4238/2015.April.28.2