Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice
Authors: X.L. Yang, Y.J. Liu, L.Q. Hu, L.W. Guo, G.P. Liu, B. Wu and H.C. Chen
The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.29
- DOI:
- 10.4238/2015.December.22.29
Genes related to high temperature tolerance during maize seed germination
Authors: S.M.F. Dutra, E.V.R. Von Pinho, H.O. Santos, A.C. Lima, R.G. Von Pinho and M.L.M. Carvalho
The identification of genes related to heat tolerance is fundamental for the development of high-quality seeds that are tolerant to heat stress condition. The objective of this study was to evaluate maize lineages and the gene expression involved in high temperature tolerance during germination using physiological tests, proteomics, and transcriptome anal.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.31
- DOI:
- 10.4238/2015.December.22.31
Sevoflurane induces neurotoxicity in young mice through FAS/FASL signaling
Authors: Q. Song, Y.L. Ma, J.Q. Song, Q. Chen, G.S. Xia, J.Y. Ma, F. Feng, X.J. Fei and Q.M. Wang
Sevoflurane, the most widely used anesthetic in clinical practice, has been shown to induce apoptosis, inhibit neurogenesis, and cause learning and memory impairment in young mice. However, the underlying mechanism is still unknown. In this study, wild-type and the FAS- or FAS ligand (FASL)-knockout mice (age 7 days) were exposed to sevoflurane or pure ox.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.32
- DOI:
- 10.4238/2015.December.22.32
Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women
Authors: W.C. Yang, L. Zhu, Y.M. Qiu, B.X. Zhou, J.L. Cheng, C.L. Wei, H.C. Chen, L.Y. Li, X.D. Fu and J.J. Fu
Non-invasive prenatal diagnosis is used to detect the genetic material of the fetus by isolating the cell-free fetal DNA (cffDNA) from maternal peripheral blood. In order to establish an isolation method for cffDNA from maternal peripheral blood in Chinese women, the cffDNA was acquired with a two-step centrifugation using a QlAamp DNA Blood mini kit. The.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.34
- DOI:
- 10.4238/2015.December.22.34
Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica)
Authors: Z.Q. Yuan, B.S. Zhao and J.Y. Zhang
We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputa.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1042
- DOI:
- 10.4238/vol10-1gmr1042
Physical location of the carotenoid biosynthesis genes Psy and �²-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae)
Authors: V. Andrade-Souza, M.G.C. Costa, C.X. Chen, F.G. Gmitter Jr. and M.A. Costa
Carotenoids are responsible for a range of fruit colors in different hot pepper (Capsicum) varieties, from white to deep red. Color traits are genetically determined by three loci, Y, C1, and C2, which are associated with carotenogenic genes. Although such genes have been localized on genetic maps of Capsicum and anchored in L.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1025
- DOI:
- 10.4238/vol10-1gmr1025
Genetic variant screening of MC3R and MC4R genes in early-onset obese children and their relatives among a Thai population: family-based study
Authors: S. Wannaiampikul, B. Phonrat, A. Tungtrongchitr, C. Limwongse, N. Chongviriyaphan, J. Santiprabhob and R. Tungtrongchitr
MC3R (melanocortin-3 receptor) and MC4R (melanocortin-4 receptor) play important roles in energy homeostasis. Severe early-onset obesity, known as monogenic obesity when it is the consequence of a mutation in a single-gene product, may result when energy homeostasis is disrupted. The purpose of our study was to screen for variations of the MC3R and MC4R g.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.35
- DOI:
- 10.4238/2015.December.22.35
Genetic characterization of papaya plants (Carica papaya L.) derived from the first backcross generation
Authors: H.C.C. Ramos, M.G. Pereira, F.F. Silva, L.S.A. Gon�§alves, F.O. Pinto, G.A. de Souza Filho and T.S.N. Pereira
The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time req.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1004
- DOI:
- 10.4238/vol10-1gmr1004
High expression of HIF-2�± and its anti-radiotherapy effect in lung cancer stem cells
Authors: J.C. Sun, F. He, W. Yi, M.H. Wan, R. Li, X. Wei, R. Wu and D.L. Niu
Hypoxia-inducible factor-2 alpha (HIF-2α) has been shown to regulate cell stemness, although the expression and effects of HIF-2α in lung cancer stem cells remained unclear. This study investigated HIF-2α expression in lung cancer stem cells, as well as the relationship between HIF-2α expression and radioresistance in lung cancer c.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.37
- DOI:
- 10.4238/2015.December.22.37
Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa
Authors: B.L. Huang, C. Cheng, G.Y. Zhang, J.J. Su, Y. Zhi, S.S. Xu, D.T. Cai, X.K. Zhang and B.Q. Huang
Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.38
- DOI:
- 10.4238/2015.December.22.38