Molecular genetic map construction and QTL analysis of fruit maturation period in grapevine
Authors: Y.H. Zhao, K. Su, Y.H. Guo, H.F. Ma and X.W. Guo
In this study, we aimed at finding the genetic regularity of grape maturation period. Early-maturing grapevine, “87-1”, was used as the female parent and late-maturing, “9-22”, as the male parent, to create an F1 hybrid population. A total of 149 individual plants and their parents were selected as the .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028040
- DOI:
- 10.4238/gmr.15028040
Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein
Authors: Y. Yi, Y.-Y. Nian, H.-W. Ji, H. Zhang, L. Zhu and Z.-W. Xu
To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriaceti.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.14.1
- DOI:
- 10.4238/2013.June.14.1
Effects of cartilage-derived morphogenetic protein 1 (CDMP1) transgenic mesenchymal stem cell sheets in repairing rabbit cartilage defects
Authors: Y. Cui, M. Yao, Y. Liu, L. Mu, B. Zhang and G. Wu
The aim of this study was to investigate the abilities of cartilage-derived morphogenetic protein 1 (CDMP1) transgenic cell sheets in repairing rabbit cartilage defects. Rabbit CDMP1 transgenic bone marrow mesenchymal stem cell (BMSC) sheets (CDMP1-BMSCs) were cultured on temperature-sensitive culture dishes, and CDMP1 expression and type II collagen prot.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028058
- DOI:
- 10.4238/gmr.15028058
Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing
Authors: A.W. Gichira, Z.C. Long, G.W. Hu, R.W. Gituru, Q.F. Wang and J.M. Chen
Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individu.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028168
- DOI:
- 10.4238/gmr.15028168
Persistent glucocorticoid resistance in systemic lupus erythematosus patients during clinical remission
Authors: A.K.G. Melo, M.R. Melo, A.B.A. Saramago, G. Demartino, B.D.B. Souza and C.A. Longui
Glucocorticoids (GCs) are key drugs in the treatment of systemic lupus erythematosus (SLE). GC dose reduction during remission is related to disease activity, GC dose used, length of treatment, and individual GC sensitivity. We compared GC receptor α (GRα) isoform and nuclear factor kappaB (NF-κB) messenger RNA quantitation and in vivo G.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.February.19.1
- DOI:
- 10.4238/2013.February.19.1
Association between ERCC1 and ERCC2 gene polymorphisms and susceptibility to pancreatic cancer
Authors: M.G. He, K. Zheng, D. Tan and Z.X. Wang
We conducted a study to investigate the association between ERCC1 (rs3212986) and ERCC2 (rs13181) gene polymorphisms and the risk of pancreatic cancer in a Chinese population. A total of 217 pancreatic cancer patients and 244 control subjects were recruited from the Nuclear Industry 215 Hospital of Shaanxi Province between February 2013 and December 2014... Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017879
- DOI:
- 10.4238/gmr.15017879
Selecting key genes associated with osteosarcoma based on a differential expression network
Authors: Y.B. Wang, N. Jia, C.M. Xu, L. Zhao, Y. Zhao, X. Wang and T.H. Jia
Despite recent advances in osteosarcoma diagnosis and therapy, much remains unclear about the molecular mechanisms involved in the disorder, and the discovery of novel drug-targeted genes is essential. We explored the potential molecular mechanisms and target genes involved in the development and progression of osteosarcoma. First, we identified the diffe.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.44
- DOI:
- 10.4238/2015.December.21.44
Wnt1 and SFRP1 as potential prognostic factors and therapeutic targets in cutaneous squamous cell carcinoma
Authors: Y. Halifu, J.Q. Liang, X.W. Zeng, Y. Ding, X.Y. Zhang, T.B. Jin, B. Yakeya, D. Abudu, Y.M. Zhou, X.M. Liu, F.X. Hu, L. Chai and X.J. Kang
The Wnt signaling pathway plays a key role in insurgence and progression of many different forms of cancer. Some crucial components of the Wnt pathway have been proposed to be novel targets for cancer therapy. To date, the Wnt signaling pathway has not been studied in cutaneous squamous cell carcinoma (CSCC). This study was de.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028187
- DOI:
- 10.4238/gmr.15028187
Correlations between traits in soybean (Glycine max L.) naturally infected with Asian rust (Phakopsora pachyrhizi)
Authors: B. Rodrigues, F. Serafim, A.P.O. Nogueira, O.T. Hamawaki, L.B. de Sousa and R.L. Hamawaki
Soybean (Glycine max L.)-breeding programs aim to develop cultivars with high grain yields and high tolerance to Asian soybean rust (Phakopsora pachyrhizi). Considering that the traits targeted for breeding are mainly quantitative in nature, knowledge of associations between traits allows the breeder to formulate indirect selection strategies. In this stu.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.45
- DOI:
- 10.4238/2015.December.21.45
Cobalt chloride induces metaphase when topically applied to larvae and pupae of the stingless bee Melipona scutellaris (Hymenoptera, Apidae, Meliponini)
Authors: C. Ueira-Vieira, R.R. Tavares, S. Morelli, B.B. Pereira, R.P. Silva, A.R. Torres-Mariano, W.E. Kerr and A.M. Bonetti
In order to optimize preparations of bee metaphases, we tested cobalt chloride, which has been used as a metaphase inducer in other organisms, such as hamsters and fish. Four microliters of 65 mM cobalt chloride aqueous solution was topically applied to larval and pupal stages of the stingless bee Melipona scutellaris. The cerebral ganglion was removed af.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.February.6.1
- DOI:
- 10.4238/2013.February.6.1