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Medical Genetics   Research Article

Influence of vascular endothelial growth factor and alpha-fetoprotein on hepatocellular carcinoma

Authors: E.Y. Yvamoto, R.F. Ferreira, V. Nogueira, M.A.S. Pinhel, G.D. Tenani, J.G.S.C. Andrade, M.E.L. Baitello, M.L. Greg�³rio, P.S. Fucuta, R.F. Silva, D.R.S. Souza and R.C.M.A. Silva

We evaluated the influence of the vascular endothelial growth factor (VEGF) -C936T polymorphism on prognosis of hepatocellular carcinoma (HCC), cirrhosis, and hepatitis C virus (HCV) infection. Serum VEGF and alpha-fetoprotein (AFP) levels were determined and used to characterize sensitivity and specificity. A total of 285 subjects were studied: 68 HCC, 1.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.16
DOI:
10.4238/2015.December.21.16
Human Genetics   Research Article

Differentiation between Triatoma arthurneivai and Triatoma wygodzinskyi (Hemiptera: Reduviidae: Triatominae) using cytotaxonomy

Authors: K.C.C. Alevi, C.H.L. Imperador, F.F.F. Moreira, J. Jurberg and M.T.V. Azeredo-Oliveira

Using classic morphometric techniques to examine the head and thorax of Triatoma specimens, researchers identified a possible taxonomic problem involving T. arthurneivai (Lent & Martins) and T. wygodzinskyi (Lent). A recent geometric morphometric study indicated that the insects captured outside the Serra do Cipó re.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027869
DOI:
10.4238/gmr.15027869
Animal Genetics   Research Article

Silkworm salivary glands are not susceptible to Bombyx mori nuclear polyhedrosis virus

Authors: L.A. Dourado, L.F.C. Ribeiro, R.M.C. Brancalh�£o, J. Tavares, A.R. Borges and M.A. Fernandez

A nuclear polyhedrosis virus isolated from infected Bombyx mori, BmNPV, was used to inoculate silkworms to determine salivary gland cell susceptibility. The salivary gland was removed from infected silkworms at different times post-inoculation and examined by light microscopy. The salivary gland cells did not exhibit any signs.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr996
DOI:
10.4238/vol10-1gmr996
Plant Genetics   Research Article

Sequence variations in the FAD2 gene in seeded pumpkins

Authors: Y. Ge, Y. Chang, W.L. Xu, C.S. Cui and S.P. Qu

Seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. The fatty acid desaturase-2 (FAD2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. However, little is known about sequence variations in FAD2 .. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.19
DOI:
10.4238/2015.December.21.19
Human Genetics   Research Article

Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)

Authors: X.J. Zhang, H.Y. Jiang, L.M. Li, L.H. Yuan and J.P. Chen

The aim of this study was to provide comprehensive insights into the genetic background of sturgeon by transcriptome study. We performed a de novo assembly of the Amur sturgeon Acipenser schrenckii transcriptome using Illumina Hiseq 2000 sequencing. A total of 148,817 non-redundant unigenes with base length of approximately 12.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027999
DOI:
10.4238/gmr.15027999
Microbial Genetics   Research Article

Endophytic and pathogenic isolates of the cacao fungal pathogen Moniliophthora perniciosa (Tricholomataceae) are indistinguishable based on genetic and physiological analysis

Authors: T.G. Lana, J.L. Azevedo, A.W.V. Pomella, R.T.R. Monteiro, C.B. Silva and W.L. Ara�ºjo

We evaluated the genetic and physiological variability of Moniliophthora perniciosa obtained from healthy and diseased branches of cacao (Theobroma cacao) plants. The diversity of the isolates was evaluated by RAPD technique and by studies of virulence and exoenzyme production. The genetic variability of endophytic and pathogenic M. perniciosa was evaluat.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr895
DOI:
10.4238/vol10-1gmr895
Human Genetics   Research Article

Genetic diversity and morphological characterization of half-sib families of Heliconia bihai L., H. chartacea Lane ex Barreiros, and H. wagneriana Peterson

Authors: F.R.A. Pereira, R.M. Moraes Filho, L.S.S. Martins, A.V.V. Montarroyos and V. Loges

The Neotropical Heliconia genus contains highly diversified plants and up to 220 species have been reported from the north of Mexico to the South of Brazil. Heliconia are cultivated as ornamental garden plants and as cut flowers. All species can be propagated by seeds or vegetatively, through rhizomes. Depending on the species.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028003
DOI:
10.4238/gmr.15028003
Bioinformatics   Research Article

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

Authors: M.P.C. Telles, F.P. Peixoto, J.S. Lima, L.V. Resende, R.P. Vianello, M.E.M.T. Walter and R.G. Collevatti

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer desig.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1026
DOI:
10.4238/vol10-1gmr1026
Microbial Genetics   Research Article

Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil

Authors: M.X. Vila Nova, L.R. Borges, A.C.B. de Sousa, B.T.R.V. Brasileiro, E.A.L.A. Lima, A.F. da Costa and N.T. de Oliveira

Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal transcribed spacers (ITS) of the rDNA region. Ten of the strains were obta.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1014
DOI:
10.4238/vol10-1gmr1014
Plant Genetics   Research Article

Differential gene expression patterns in the autogamous plant Hordeum euclaston (Poaceae)

Authors: J.E. Georg-Kraemer, C.A.S. Ferreira and S.S. Cavalli

Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carri.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1017
DOI:
10.4238/vol10-1gmr1017