Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil
Authors: Q.L. Barcelos, E.A. Souza and K.J. Damasceno e Silva
The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibili.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr907
- DOI:
- 10.4238/vol10-1gmr907
Expression of recombinant human anti-TNF-�± scFv-Fc in Arabidopsis thaliana seeds
Authors: N. Yao, L. Ai, Y.Y. Dong, X.M. Liu, D.Z. Wang, N. Wang, X.W. Li,F.W. Wang, XK. Li, H.Y. Li and C. Jiang
Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest si.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027726
- DOI:
- 10.4238/gmr.15027726
Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)
Authors: S. Ghosh, P.B. Majumder and S. Sen Mandi
The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most imp.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1154
- DOI:
- 10.4238/vol10-1gmr1154
Agronomic characterization, genetic diversity and association analysis of cotton cultivars using simple sequence repeat molecular markers
Authors: A. Kalivas, F. Xanthopoulos, O. Kehagia and A.S. Tsaftaris
Cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. Because of its worldwide economic importance, new cultivars are constantly being released in the world and consequently in the Greek market, as Greece is the largest producer in Europe. We used simple s.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr998
- DOI:
- 10.4238/vol10-1gmr998
O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines
Authors: G.G.N. de S. Wisintainer, G. Scola, S. Moura, T.L.G. Lemos, C. Pessoa, M.O. de Moraes, L.G.S. Souza, M. Roesch-Ely and J.A.P. Henriques
Biflorin is an o-naphthoquinone isolated from the roots of the plant Capraria biflora L. (Scrophulariaceae). In this study, the cytotoxic effects of biflorin were verified, and late apoptosis was detected in various cancer cell lines by in situ analysis. The cytotoxicity was further evaluated exclusively for 48 h of treatment in different tumor and non-tu.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.18
- DOI:
- 10.4238/2015.December.21.18
Development and characterization of microsatellite markers for the walking goby (Scartelaos viridis; Gobiidae)
Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang
Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1015
- DOI:
- 10.4238/vol10-1gmr1015
Identification of SNPs within the PRLR gene and effects on maternal behavior in sheep
Authors: L.P. Wang, R.Q. Geng, X.N. Zhang and W. Sun
The prolactin receptor gene (PRLR) plays an essential role in maternal behavior. The aim of the study was to detect PRLR mutations in exon 10, using a polymerase chain reaction-single stranded conformation polymorphism method, and to determine the association between mutations in this region with maternal behavior traits in Chinese Hu sheep. Polymorphisms.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.26
- DOI:
- 10.4238/2015.December.21.26
Genetic parameters and mapping quantitative trait loci associated with tibia traits in broilers
Authors: B.N.N. Ragognetti, N.B. Stafuzza, T.B.R. Silva, T.C.S. Chud, N.V. Grupioni, V.A.R. Cruz, J.O. Peixoto, K. Nones, M.C. Ledur and D.P. Munari
Selection among broilers for performance traits is resulting in locomotion problems and bone disorders, once skeletal structure is not strong enough to support body weight in broilers with high growth rates. In this study, genetic parameters were estimated for body weight at 42 days of age (BW42), and tibia traits (length, width, and weight) in a populati.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.27
- DOI:
- 10.4238/2015.December.21.27
Expression characteristics of MMP-2 and MMP-9 in guinea pig ovaries during the estrous cycle
Authors: J.R. Li and T. Shen
We investigated the expression characteristics of matrix metalloproteinases (MMP)-2 and MMP-9 in the ovaries of normal adult guinea pigs during the estrous cycle. The estrous cycle was classified according to the appearance of the vaginal orifice and by vaginal exfoliated cell smears. Guinea pigs that had experienced at least two normal estrous cycles wer.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.1
- DOI:
- 10.4238/2015.December.21.1
Polymorphisms of GSTM1, GSTT1, and p53 in Goi�¢nia, Goi�¡s
Authors: D.A. Rodrigues, I.R. Costa, J.V.M. Martins, K.S.F. e Silva and K.K.V.O. Moura
Genetic polymorphisms are defined as changes within the DNA sequences of genes that have frequencies in the population higher than 1%. The glutathione S-transferases play an important role in the cellular detoxification systems involved in oxidative stress that can lead to accumulation of reactive oxygen species. Epidemiological studies have suggested tha.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.4
- DOI:
- 10.4238/2015.December.21.4