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Plant Genetics   Research Article

Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)

Authors: S. Ghosh, P.B. Majumder and S. Sen Mandi

The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most imp.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1154
DOI:
10.4238/vol10-1gmr1154
Plant Genetics   Research Article

Agronomic characterization, genetic diversity and association analysis of cotton cultivars using simple sequence repeat molecular markers

Authors: A. Kalivas, F. Xanthopoulos, O. Kehagia and A.S. Tsaftaris

Cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. Because of its worldwide economic importance, new cultivars are constantly being released in the world and consequently in the Greek market, as Greece is the largest producer in Europe. We used simple s.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr998
DOI:
10.4238/vol10-1gmr998
Plant Genetics   Research Article

O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines

Authors: G.G.N. de S. Wisintainer, G. Scola, S. Moura, T.L.G. Lemos, C. Pessoa, M.O. de Moraes, L.G.S. Souza, M. Roesch-Ely and J.A.P. Henriques

Biflorin is an o-naphthoquinone isolated from the roots of the plant Capraria biflora L. (Scrophulariaceae). In this study, the cytotoxic effects of biflorin were verified, and late apoptosis was detected in various cancer cell lines by in situ analysis. The cytotoxicity was further evaluated exclusively for 48 h of treatment in different tumor and non-tu.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.18
DOI:
10.4238/2015.December.21.18
Bioinformatics   Short communication

Development and characterization of microsatellite markers for the walking goby (Scartelaos viridis; Gobiidae)

Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang

Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1015
DOI:
10.4238/vol10-1gmr1015
Animal Genetics   Research Article

Identification of SNPs within the PRLR gene and effects on maternal behavior in sheep

Authors: L.P. Wang, R.Q. Geng, X.N. Zhang and W. Sun

The prolactin receptor gene (PRLR) plays an essential role in maternal behavior. The aim of the study was to detect PRLR mutations in exon 10, using a polymerase chain reaction-single stranded conformation polymorphism method, and to determine the association between mutations in this region with maternal behavior traits in Chinese Hu sheep. Polymorphisms.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.26
DOI:
10.4238/2015.December.21.26
Animal Genetics   Research Article

Genetic parameters and mapping quantitative trait loci associated with tibia traits in broilers

Authors: B.N.N. Ragognetti, N.B. Stafuzza, T.B.R. Silva, T.C.S. Chud, N.V. Grupioni, V.A.R. Cruz, J.O. Peixoto, K. Nones, M.C. Ledur and D.P. Munari

Selection among broilers for performance traits is resulting in locomotion problems and bone disorders, once skeletal structure is not strong enough to support body weight in broilers with high growth rates. In this study, genetic parameters were estimated for body weight at 42 days of age (BW42), and tibia traits (length, width, and weight) in a populati.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.27
DOI:
10.4238/2015.December.21.27
Animal Genetics   Research Article

Expression characteristics of MMP-2 and MMP-9 in guinea pig ovaries during the estrous cycle

Authors: J.R. Li and T. Shen

We investigated the expression characteristics of matrix metalloproteinases (MMP)-2 and MMP-9 in the ovaries of normal adult guinea pigs during the estrous cycle. The estrous cycle was classified according to the appearance of the vaginal orifice and by vaginal exfoliated cell smears. Guinea pigs that had experienced at least two normal estrous cycles wer.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.1
DOI:
10.4238/2015.December.21.1
Microbial Genetics   Research Article

Polymorphisms of GSTM1, GSTT1, and p53 in Goi�¢nia, Goi�¡s

Authors: D.A. Rodrigues, I.R. Costa, J.V.M. Martins, K.S.F. e Silva and K.K.V.O. Moura

Genetic polymorphisms are defined as changes within the DNA sequences of genes that have frequencies in the population higher than 1%. The glutathione S-transferases play an important role in the cellular detoxification systems involved in oxidative stress that can lead to accumulation of reactive oxygen species. Epidemiological studies have suggested tha.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.4
DOI:
10.4238/2015.December.21.4
Plant Genetics   Research Article

Isolation of genomic DNA from defatted oil seed residue of rapeseed (Brassica napus)

Authors: M. Sadia, M.A. Rabban, S. Hameed, S.R. Pearce and S.A. Malik

A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual bioma.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1009
DOI:
10.4238/vol10-1gmr1009
Human Genetics   Research Article

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

Authors: M.M. Castro, I.P. Farias, C.M. Borborema-Santos, G. Correia and S. Astolfi-Filho

Infection by human papillomavirus (HPV) is one of the primary causes of mortality by cancer in northern Brazil. Sexually active women from Manaus, Amazonas, without cytological alterations and women with pre-malignant and malignant cytological alterations were examined for HPV virus, identified via PCR and sequencing. The targ.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr992
DOI:
10.4238/vol10-1gmr992