Human kallikrein 5 as a novel prognostic biomarker for triple-negative breast cancer: tissue expression analysis and relationship with disease course
Authors: F. Yang, J.Y. Li, Q.N. Yin, K. Yang, S.N. Dong, L.J. Bai, P. Liu and X.W. Tong
The purposes of this study were to analyze the expression and distribution of human kallikrein 5 (hK5) in triple-negative breast cancer (TNBC) tissues, to establish a standard operating procedure (SOP) for its immunohistochemical assay, and to evaluate the possibility of hK5 being a prognostic biomarker for TNBC. Recombinant hK5 protein and specific antib.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.28
- DOI:
- 10.4238/2015.August.14.28
Genetic parameters and breeding strategies for high levels of iron and zinc in Phaseolus vulgaris L.
Authors: S.M. Martins, P.G.S. Melo, L.C. Faria, T.L.P.O. Souza, L.C. Melo and H.S. Pereira
One of the current focus of common bean breeding programs in Brazil is to increase iron (FeC) and zinc content (ZnC) in grains. The objectives of this study were to estimate genetic parameters for FeC and ZnC in common bean, verify the need for conducting multi-site evaluation tests, identify elite lines that combine high FeC .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028011
- DOI:
- 10.4238/gmr.15028011
Analysis of the genetic diversity of beach plums by simple sequence repeat markers
Authors: X.M. Wang, W.L. Wu, C.H. Zhang, Y.P. Zhang, W.L. Li and T. Huang
The purpose of this study was to measure the genetic diversity of wild beach plum and cultivated species, and to determine the species relationships using SSRs markers. An analysis of genetic diversity from ten beach plum germplasms was carried out using 11 simple sequence repeat (SSR) primers selected from 35 primers to generate distinct PCR products. Fr.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.2
- DOI:
- 10.4238/2015.August.19.2
In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops
Authors: C. Bredow, J.L. Azevedo, J.A. Pamphile, C.A. Mangolin and S.A. Rhoden
Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.3
- DOI:
- 10.4238/2015.August.19.3
Isolation and characterization of a novel paraffin wax-degrading bacterium, Pseudomonas sp strain PW-1, from petroleum-contaminated sites
Authors: Y.L. Zhang, Z. Liu, and T. Liu
An isolate capable of degrading paraffin wax was isolated from petroleum-contaminated sites in Daqing, China, and identified as Pseudomonas sp strain PW-1 by analyzing the 16S rDNA sequence (GenBank accession No.: KF529529) as well as the biochemical and physiological characteristics. The optimized degradation conditions of th.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028021
- DOI:
- 10.4238/gmr.15028021
Relationship between zinc and the growth and development of young children
Authors: S. Gao, D.N. Tu, H. Li, X. Cao, J.X. Jiang, Y. Shi, X.Q. Zhou1and J.B. You
The purpose of this study was to evaluate the relationship between zinc and the growth and development of young children. The parents of 8102 young children were surveyed in person by a trained surveyor using structured questionnaires. The hair zinc concentration of the children was determined using an atomic absorption spectrophotometer. The height, weig.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.5
- DOI:
- 10.4238/2015.August.19.5
Genetic diversity and structure of the threatened species Sinopodophyllum hexandrum (Royle) Ying
Authors: W. Liu, J. Wang, D.X. Yin, M. Yang, P. Wang, Q.S. Han, Q.Q. Ma, J.J. Liu and J.X. Wang
Sinopodophyllum hexandrum is an important medicinal plant that has been listed as an endangered species, making the conservation of its genetic diversity a priority. Therefore, the genetic diversity and population structure of S. hexandrum was investigated through inter-simple sequence repeat analysis of eight natural populati.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028130
- DOI:
- 10.4238/gmr.15028130
Effects of acupotomylysis on basic fibroblast growth factor and CD34 levels in rabbits with third lumbar vertebral transverse foramen syndrome
Authors: X.-H. Li, N.-G. Liu, C.-Q. Guo, H.-M. Sun, H.-X. Wu, H. Xu and Y. Zhang
This study observed the local tissue homogenates in rabbits with third lumbar vertebral transverse foramen syndrome and explored the mechanism of acupotomylysis in local tissue revascularization. Thirty Japanese white rabbits were randomly divided into the following 5 groups of 6 rabbits each: normal, model, acupotomy, electroacupuncture (EA), and acupoto.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.6
- DOI:
- 10.4238/2015.August.19.6
Association between transcription factor 7-like 2 genetic polymorphisms and development of type 2 diabetes in a Chinese population
Authors: H.Y. Jia, Q.Z. Li and L.F. Lv
We conducted a hospital-based case-control study to evaluate the relationship between the transcription factor 7-like 2 (TCF7L2) rs7903146 polymorphism and type 2 diabetes mellitus risk in a Chinese population. Genotyping of TCF7L2 rs7903146 was carried out using the polymerase chain reaction-restriction fragment length polymo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028223
- DOI:
- 10.4238/gmr.15028223
Identification of target genes for adenohypophysis-prefer miR-7 and miR-375 in cattle
Authors: B. Yuan, G.J. Sun, G.L. Zhang, J. Wu, C. Xu, L.S. Dai, J. Chen, X.F. Yu, Z.H. Zhao and J.B. Zhang
In this study, expression levels of miRNAs (miRNAs), miR-375 and miR-7, were detected in different tissues of cattle to determine whether adenohypophysis-prefer or exclusively expressed miRNAs, and target genes could be predicted by TargetScan, RNA22, and other software. Target genes related to pituitary function or reproductive traits were identified usi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.8
- DOI:
- 10.4238/2015.August.19.8