Correlation between Y chromosome microdeletion and male infertility
Authors: X.G. Liu, H.Y. Hu, Y.H. Guo and Y.P. Sun
Dyszoospermia due to genetic factors is the leading cause of male infertility. To explore the correlation between azoospermia factor (AZF) microdeletion of the Y chromosome and male infertility, we evaluated AZF microdeletion on the long arm of the Y chromosome in 166 infertile males and 50 fertile males using multiplex polyme.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028426
- DOI:
- 10.4238/gmr.15028426
Downregulation of MACC1 expression enhances cisplatin sensitivity in SKOV-3/DDP cells
Authors: Z.M. Chen, H.R. Shi, X. Li, Y.X. Deng and R.T. Zhang
The aim of this study was to investigate the correlation between MACC1 expression and resistance to cisplatin (DDP) in DDP-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP). MACC1 mRNA and protein expression levels in SKOV-3 and SKOV-3/DDP cells were detected by reverse transcriptase polymerase chain reaction and western blot. The SKOV-3.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.13
- DOI:
- 10.4238/2015.December.16.13
Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus
Authors: P.-Z. Qi, C.-X. Xie, B.-Y. Guo, C.-W. Wu, S.-M. Lu and Y.-J. Duan
Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polym.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.21.7
- DOI:
- 10.4238/2013.May.21.7
Breeding value accuracy estimates for growth traits using random regression and multi-trait models in Nelore cattle
Authors: A.A. Boligon, F. Baldi, M.E.Z. Mercadante2, R.B. Lobo, R.J. Pereira and L.G. Albuquerque
We quantified the potential increase in accuracy of expected breeding value for weights of Nelore cattle, from birth to mature age, using multi-trait and random regression models on Legendre polynomials and B-spline functions. A total of 87,712 weight records from 8144 females were used, recorded every three months from birth .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1087
- DOI:
- 10.4238/vol10-2gmr1087
A SCAR marker for the analysis of chloroplast DNA from different cultivars of Cornus officinalis
Authors: L. Zhang, R.X. Liu, J. Wang, T. Zhang, J. Li, J.H. Shi, B.Y. Kang and S.Q. Chen
The aims of this study were to establish a random amplified polymorphic DNA (RAPD) fingerprint database of chloroplast DNA (cpDNA) from different cultivars of Cornus officinalis and to convert RAPD markers to sequence characterized amplified regions (SCAR) markers. A method of extraction was established that was suitable for obtaining cpDNA from samples r.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.17
- DOI:
- 10.4238/2015.December.16.17
Correlation between polymorphisms in the glucocorticoid receptor gene NR3C1 and susceptibility to asthma in a Chinese population from the Henan Province
Authors: Z. Cheng, L.L. Dai, Q. Liu, M. Liu, Q. Wang, P.F. Li, H. Wang, L.Q. Jia and L. An
The aim of this study was to investigate the association between four single nucleotide polymorphisms in NR3C1 (Tth111I, BclI, ER22/23EK, and N363S), which encode the glucocorticoid receptor, and asthma susceptibility in patients from the Henan Province of China. Three hundred and twenty-eight patients with asthma and 60 healt.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028507
- DOI:
- 10.4238/gmr.15028507
Application of denaturing gradient gel electrophoresis for detection of bacterial and yeast communities along a salinity gradient in the estuary of the Cachoeira River in Brazil
Authors: C.S.P. Rodrigues, S.S. Souza, R.P. Rezende, A. Silva, J.L. Andrioli, H. Costa, R. Fontana and J.C.T. Dias
An estuary is a transition zone between freshwater and marine ecosystems, resulting in dilution of seawater. Estuaries are also considered environments of intense biological activity related to the processes of nutrient cycling. The aim of this study was to evaluate the microbial community composition along a salinity gradient in the estuary of the Cachoe.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.21.6
- DOI:
- 10.4238/2013.May.21.6
Regulation network of serum cytokines induced by tuberculosis-specific antigens reveals biomarkers for tuberculosis diagnosis
Authors: M. Wei, Z.Y. Wu, J.H. Lin, Y. Li, Z.X. Qian, Y.Q. Xie, H. Su and W. Zhou
In this study, we identified potential serum biomarkers for the diagnosis of active tuberculosis (TB) and screening for latent TB infections (LTBIs). Peripheral blood samples from 40 healthy individuals, 40 patients with TB, and 40 LTBI individuals were stimulated with the TB-specific antigens ESAT-6 and CFP-10. Human inflammatory cytokine arrays were use.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.18
- DOI:
- 10.4238/2015.December.16.18
Association between N-acetyltransferase 2 polymorphisms and pancreatic cancer risk: a meta-analysis
Authors: J.X. Liang, W. Gao, Y. Liang and X.M. Zhou
N-acetyltransferase 2 (NAT2) is an essential phase II enzyme in the metabolism of aromatic and heterocyclic amines and of hydrazines. NAT2 activity can be divided into three phenotypes: rapid, intermediate, and slow. Studies identifying an association between NAT2 polymorphism and the risk of pancreatic cancer have shown conflicting results. In order to a.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.21
- DOI:
- 10.4238/2015.December.16.21
Distribution of LIM domain kinase 1 in the olfactory bulb, cerebral cortex, hippocampus, and cerebellum of the App/PS+/- mice
Authors: L. An, J. Liu, W.W. Li, Y.J. Shi, X.H. Lin, D.M. Yu, J.B. Deng and X.Q. Ren
LIM domain kinase 1 (LIMK1), an actin-binding kinase, can phosphorylate and inactivate its substrates, and can regulate long-term memory and synaptic plasticity. Both β-amyloid precursor protein (App) and presenilin (PS) are functional degeneration factors during early neuronal development, and are considered as potential factors that contribute to t.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.24
- DOI:
- 10.4238/2015.December.16.24