Altered callose deposition during embryo sac formation of multi-pistil mutant (mp1) in Medicago sativa
Authors: H.C. Zhou, L. Jin, J. Li and X.J. Wang
Whether callose deposition is the cause or result of ovule sterility in Medicago sativa remains controversial, because it is unclear when and where changes in callose deposition and dissolution occur during fertile and sterile embryo sac formation. Here, alfalfa spontaneous multi-pistil mutant (mp1) and wild-type plants were u.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027968
- DOI:
- 10.4238/gmr.15027968
Identification of expressed sequences in the coffee genome potentially associated with somatic embryogenesis
Authors: A.T. Silva, L.V. Paiva, A.C. Andrade and D. Barduche
Brazil possesses the most modern and productive coffee growing farms in the world, but technological development is desired to cope with the increasing world demand. One way to increase Brazilian coffee growing productivity is wide scale production of clones with superior genotypes, which can be obtained with in vitro propagation technique, or from tissue.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.21.1
- DOI:
- 10.4238/2013.May.21.1
Genetic diversity in mutated and non-mutated rice varieties
Authors: M. Naeem, F. Ghouri, M.Q. Shahid, M. Iqbal, F. S. Baloch, L. Chen, S. Allah, M. Babar and M. Rana
We studied the genetic variability due to mutation induced by γ-rays (10, 15, and 20 Kr) on various traits of twelve rice genotypes. Mutated and non-mutated seeds were sown in the field between July 2013 and 2014 using a split plot design. Yield and yield-related trait data was recorded, which showed significant (P < 0.05) genotypic and irradiati.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.11
- DOI:
- 10.4238/2015.December.16.11
Cloning and functional analysis of goat SWEET1
Authors: L.Q. Zhu, Z.K. Bao, W.W. Hu, J. Lin, Q. Yang and Q.H. Yu
SWEETs are a recently discovered class of sugar transporters that mediate glucose uptake in the intestine and mammary glands. Our objectives were to clone goat SWEET1 and conduct a functional analysis of its effect on glucose efflux in goat mammary gland epithelial cells. We cloned and sequenced the goat SWEET1 gene from goat mammary glands, then conducte.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.12
- DOI:
- 10.4238/2015.December.16.12
MCP-1 gene polymorphisms in North Chinese patients with pulmonary tuberculosis
Authors: G.L. Shi,L. Yang,Y. Sun,Y.J. Yin and C.X. Song
Pulmonary tuberculosis (PTB) remains one of the most important infectious diseases worldwide. Several studies have suggested that genetic factors may affect the susceptibility to PTB, but the specific genes involved have not been fully characterized. The gene for monocyte chemoattractant protein 1 (MCP-1) has been linked to an increased risk of tuberculos.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.18
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.18
Isolation and characterization of polymorphic microsatellite markers for blue fox (Alopex lagopus)
Authors: Y.M. Li, P.C. Guo, J.Y. Lu, C.Y. Bai, Z.H. Zhao and S.Q. Yan
The blue fox, belonging to the family Canidae, is a coat color variant of the native arctic fox (Alopex lagopus). To date, microsatellite loci in blue fox are typically amplified using canine simple sequence repeat primers. In the present study, we constructed an (AC)n enrichment library, and isolated and identified 17 polymor.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027990
- DOI:
- 10.4238/gmr.15027990
Effect of siRNA targeting HER2/neu on the proliferation and viability of prostate cancer PC-3M cells
Authors: C.Y. Liu, P.C. Xu, D.G. Chen, X.H. Fan, M.Q. Li, X. Yang and Y.P. Xu
The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposom.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.14
- DOI:
- 10.4238/2015.December.16.14
Microarray profiles of ex vivo expanded hematopoietic stem cells show induction of genes involved in noncanonical Wnt signaling
Authors: D.L. Zanette, J.C.C. Lorenzi, R.A. Panepucci, A.R.D. Santos, G.A. Molfetta, A.G. Araujo, W.A. Silva Junior and M.A. Zago
The low number of hematopoietic stem cells (HSC) in umbilical cord blood (UCB) is directly related to increased risk of transplant failure. Effective ex vivo expansion of HSC has been tried for many years, with conflicting results because of the inability to reproduce in vitro HSC proliferation in the same way it occurs in vivo. We compared freshly isolat.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.15.1
- DOI:
- 10.4238/2013.May.15.1
Analysis of genetic diversity of Laeliinae (Orchidaceae) in the State of Sergipe using ISSR markers
Authors: M.F. Arrigoni-Blank, M.S. Santos, A.F. Blank, A.R.C. Rabbani, R. Silva-Mann, J.B. Santos, A.S. Costa and T.S.A. Menezes
The Orchidaceae represent one of the largest and most diverse families on the planet. However, this family is constantly threatened by predators and by the advancement of urban centers over its natural habitats. The objective of this study was to use inter-simple sequence repeat markers to evaluate the genetic diversity betwee.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027997
- DOI:
- 10.4238/gmr.15027997
New polymorphic microsatellite loci of threadsail filefish, Stephanolepis cirrhifer (Teleostei, Monacanthidae), from Korean waters
Authors: C.-M. An, H.S. An, J.W. Lee and S.W. Hong
The threadsail filefish, Stephanolepis cirrhifer (Monacanthidae), is found mainly in the western Pacific. It is intensively caught in Korea and is a highly appreciated seafood delicacy. Consequently, the natural population of this species has drastically decreased, despite introductions from hatcheries. To provide information necessary for its conservatio.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.14.8
- DOI:
- 10.4238/2013.May.14.8