Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG
Authors: J. Ma, M.G. Wang, A.H. Mao, J.Y. Zeng, Y.Q. Liu, X.Q. Wang, J. Ma, Y.J. Tian, N. Ma, N. Yang, L. Wang and S.Q. Liao
Aptamers that recognize the IgG Fc region are of great interest because of their wide application as an immunology probing tool, for diagnostics, and as affinity agents for antibody purification. We developed a target replacement strategy as a modification of conventional Systematic Evolution of Ligands by EXponential enrichment (SELEX) in order to effici.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.11
- DOI:
- 10.4238/2013.April.25.11
Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense
Authors: N.M.A. Rahman, H. Fu, H. Qiao, S. Jin, H. Bai, W. Zhang, F.W. Jiang, G. Liang, S. Sun, Y. Gong, F.F. Jiang, Y. Xiong and Y. Wu
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different deve.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027950
- DOI:
- 10.4238/gmr.15027950
Differential expression of lipid metabolism genes in the liver and adipose tissue of mice treated with evodiamine
Authors: D.F. Jiang, X.G. Zhang, H.L. Yang and C. Sun
Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously inj.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.January.4.13
- DOI:
- 10.4238/2013.January.4.13
Selection in sugarcane based on inbreeding depression
Authors: A.A.C. de Azeredo, L.L. Bhering, B.P. Brasileiro, C.D. Cruz and M.H.P. Barbosa
This study aimed to evaluate the gene action associated with yield-related traits, including mean stalk weight (MSW), tons of sugarcane per hectare (TCH), and fiber content (FIB) in sugarcane. Moreover, the viability of individual reciprocal recurrent selection (RRSI-S1) was verified, and the effect of inbreeding depression on.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027965
- DOI:
- 10.4238/gmr.15027965
CYP2C19 polymorphisms in acute coronary syndrome patients undergoing clopidogrel therapy in Zhengzhou population
Authors: Y.M. Guo, Z.C. Zhao, L. Zhang, H.Z. Li, Z. Li and H.L. Sun
The goal of this study was to explore the polymorphisms of CYP2C19 (CYP2C19*2, CYP2C19*3) in patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) on clopidogrel therapy in Zhengzhou city for guidance on clinical medication and reduction in the incidence of thromboembolic events. Two h.. Read More»
- Genet. Mol. Res. 15(2):
Genetic polymorphisms in patients with endometriosis: an analytical study in Goi�¢nia (Central West of Brazil)
Authors: K.S.F. Silva and K.K.V.O. Moura
In healthy women, intra- and extracellular controls prevent the attachment and proliferation of ectopic endometrial cells. During endometriosis, abnormalities in these control mechanisms permit the survival of endometrial cells, their subsequent attachment to the peritoneal cavity, and disease progression. These abnormal cells.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028135
- DOI:
- 10.4238/gmr.15028135
Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae
Authors: C.M. Li, B.M. Fu, L.C. Zhang, B. Tang, L. Zhu, Y. Zhao and J. Zhang
Currently, there is no practical and efficient method for the isolation of bone marrow cells (BMCs) from rat femurs and tibiae. Here, we attempted to develop a rapid, simple, effective, and non-contaminating method for the isolation of BMCs from rat femurs and tibiae. Rat femurs and tibiae were dissected from the ankle to the .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028178
- DOI:
- 10.4238/gmr.15028178
Bioequivalence of clopidogrel hydrogen sulfate tablets in healthy Chinese volunteers
Authors: Y.G. Yang, M. Zhang, N. Jiang, L.X. Song, X.T. Xu, X.H. Di, L. Xu, J. Xu and G.T. Zhao
We aimed to evaluate the bioequivalence of clopidogrel in healthy Chinese volunteers after administration of a single oral dose. We administered a single oral dose of 75 mg clopidogrel (test and reference) to 32 healthy Chinese volunteers according to an open, randomized, crossover design. The concentration of clopidogrel acid (carboxylic metabolite of cl.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December14.1
- DOI:
- 10.4238/2015.December14.1
Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle
Authors: S. Wohlres-Viana, E.K.N. Arashiro, D.R.L. Reis, L.E. Fernandes, M.G.C.D. Peixoto, M.A. Machado and J.H.M. Viana
The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. C.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027046
- DOI:
- 10.4238/gmr.15027046
Isolation and characterization of Calcineurin B-like gene (PbCBL1) and its promoter in birch-leaf pear (Pyrus betulifolia Bunge)
Authors: Y.Y. Xu, H. Li, J. Lin, X.G. Li and Y.H. Chang
Calcium plays a critical role in regulating abiotic stress responses in plants. Calcineurin B-like (CBL) proteins are calcium sensors in calcium signaling pathways. However, the molecular mechanisms underlying calcium signaling remain to be elucidated. In this study, the CBL1 gene, which codes for the CBL protein, was isolated from the birch-leaf pear. On.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.3
- DOI:
- 10.4238/2015.December.14.3