Regulation of CD4+FOXP3+ T cells by CCL20/CCR6 axis in early unexplained recurrent miscarriage patients
Authors: X.X. Zhang, X.M. Kang and A.M. Zhao
Expression and function of CCR6/CCL20 in CD4+FOXP3+ regulatory T cells (Tregs) was investigated in unexplained recurrent miscarriage (URM) patients. Flow cytometry, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, western blots, and Transwell migration assays were used to analyze the expression and function of regulatory.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.24
- DOI:
- 10.4238/2015.August.7.24
Models of logistic regression analysis, support vector machine, and back-propagation neural network based on serum tumor markers in colorectal cancer diagnosis
Authors: B. Zhang, X.L. Liang, H.Y. Gao, L.S. Ye and Y.G. Wang
We evaluated the application of three machine learning algorithms, including logistic regression, support vector machine and back-propagation neural network, for diagnosing congenital heart disease and colorectal cancer. By inspecting related serum tumor marker levels in colorectal cancer patients and healthy subjects, early d.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028643
- DOI:
- 10.4238/gmr.15028643
PDIA3 and PDIA6 gene expression as an aggressiveness marker in primary ductal breast cancer
Authors: F.S. Ramos, L.T.R. Serino, C.M.S. Carvalho, R.S. Lima, C.A. Urban, I.J. Cavalli and E.M.S.F. Ribeiro
Changes in the expression of the protein disulfide isomerase genes PDIA3 and PDIA6 may increase endoplasmic reticulum stress, leading to cellular instability and neoplasia. We evaluated the expression of PDIA3 and PDIA6 in invasive ductal carcinomas. Using reverse transcription-quantitative polymerase chain reaction, we compared the mRNA expression level .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.4
- DOI:
- 10.4238/2015.June.26.4
Effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5
Authors: Z.R. Xu, F.G. Jiang and F. Chen
The OPTN gene is thought to be associated with certain types of glaucoma and the function of the protein for which it encodes, optineurin, has been extensively researched, but with contradictory results. We explored the effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5. Plasmids expressing wild-type (WT).. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.27
- DOI:
- 10.4238/2015.August.7.27
Inhibition of adipogenic differentiation of bone marrow mesenchymal stem cells by erythropoietin via activating ERK and P38 MAPK
Authors: G.X. Liu, J.C. Zhu, X.Y. Chen, A.Z. Zhu, C.C. Liu, Q. Lai and S.T. Chen
We examined whether erythropoietin (EPO) can inhibit adipogenic differentiation of mesenchymal stem cells (MSCs) in the mouse bone marrow and its underlying mechanism. We separated and extracted mouse bone marrow MSCs and induced adipogenic differentiation using 3-isobutyl-1-methylxanthine, insulin, and dexamethasone. Different concentrations of EPO were .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.5
- DOI:
- 10.4238/2015.June.26.5
Isolation, identification, and optimal cultivation of a marine bacterium antagonistic to Magnaporthe grisea
Authors: W.J. Zhang, P. Guo, M. Liu, B.L. Yang, J.H. Wang and J. Jiang
In this paper, a plate confrontation method was used to isolate bacteria antagonistic to the rice blast fungus Magnaporthe grisea from samples collected from China’s Dalian Bay. The antagonist strain LM-031 was obtained. We studied this strain’s morphological, physiological and biochemical characteristics and analy.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028646
- DOI:
- 10.4238/gmr.15028646
Genome-scale meta-analysis of DNA methylation during progression of lung adenocarcinoma
Authors: Q.H. Zhang, X.H. Dai, Z.M. Dai and Y.N. Cai
Identification of epigenetic alterations in tumors has become a common method for identifying genes critical to cancer development and progression. Thus, we identified DNA methylation alterations on the genome scale during lung adenocarcinoma (LADC) progression to understand the carcinogenic process and identify clinically relevant biomarkers. We found th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.30
- DOI:
- 10.4238/2015.August.7.30
Serum immunoglobulin E level and its impact on the pregnancy outcome associated with fetal growth restriction: a prospective cohort study
Authors: F. Xiong,Y. Tong,P. Li,T. Huo and M. Mao
We evaluated the relationship between total serum immunoglobulin E (IgE) levels and pregnancy outcome in a prospective cohort study focusing on fetal growth restriction (FGR). Sixty women with FGR and twenty with normal singleton pregnancy were enrolled during their third trimester. Infants were followed up for 6 months. Blood samples were obtained from p.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.2
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.2
Opg/Rankl mRNA dynamic expression in the bone tissue of ovariectomized rats with osteoporosis
Authors: C.W. Li, B. Liang, X.L. Shi and H. Wang
We established animal models of osteoporosis in ovariectomized rats to detect osteoprogerin (Opg)/receptor activator of nuclear factor-κB ligand (Rankl) mRNA expression levels in the tibias and serum estradiol concentrations at different time points. Sixty Sprague-Dawley female rats were randomly selected and divided into an ovariectomized.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.1
- DOI:
- 10.4238/2015.August.10.1
Analysis of regulatory mechanism after ErbB4 gene mutation based on local modeling methodology
Authors: C.L. Chen and J.W. Zhao
ErbB4 is an oncogene belonging to the epidermal growth factor receptor family and contributes to the occurrence and development of multiple cancers, such as gastric, breast, and colorectal cancers. Therefore, studies of the regulation of ErbB4 in cancerigenic pathway will advance molecular targeted therapy. Advanced bioinforma.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028647
- DOI:
- 10.4238/gmr.15028647