Isolation of a novel lipase from a metagenomic library derived from mangrove sediment from the south Brazilian coast
Authors: G.H. Couto, A. Glogauer, H. Faoro, L.S. Chubatsu, E.M. Souza and F.O. Pedrosa
A novel gene coding for a LipA-like lipase with 283 amino acids and a molecular mass of 32 kDa was isolated and characterized from a metagenomic library prepared from mangrove sediment from the south Brazilian coast. LipA was 52% identical to a lipolytic enzyme from an uncultured bacterium and shared only low identities (≤3.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr738
- DOI:
- 10.4238/vol9-1gmr738
Assessment of clinical scoring systems for the diagnosis of Williams-Beuren syndrome
Authors: D.E.S. Leme, D.H. Souza, G. Mercado, E. Pastene, A. Dias and D. Moretti-Ferreira
Williams-Beuren syndrome (WBS) is a genetic disorder characterized by physical and intellectual developmental delay, associated with congenital heart disease and facial dysmorphism. WBS is caused by a microdeletion on chromosome 7 (7q11.23), which encompasses the elastin (ELN) gene and about 27 other genes. The gold standard for WBS laboratory diagnosis i.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.7
- DOI:
- 10.4238/2013.September.4.7
Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle
Authors: Y.L. He, Y.H. Wu, F.S. Quan, Y.G. Liu and Y. Zhang
To better understand the function of the myostatin gene and its promoter region in bovine, we amplified and sequenced the myostatin gene and promoter from the blood of Qinchuan and Red Angus cattle by using polymerase chain reaction. The sequences of Qinchuan and Red Angus cattle were compared with those of other cattle breeds available in GenBank. Exon s.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.6
- DOI:
- 10.4238/2013.September.4.6
Role of a homozygous A(TA)7TAA promoter polymorphism and an exon 1 heterozygous frameshift mutation UGT1A1 in Crigler-Najjar syndrome type II in a Thai neonate
Authors: P. Nilyanimit, A. Krasaelap, M. Foonoi, V. Chongsrisawat and Y. Poovorawan
Crigler-Najjar syndrome is a rare autosomal recessive disease caused by mutations in the UGT1A1 gene. These mutations result in the deficiency of UGT1A1, a hepatic enzyme essential for bilirubin conjugation. This report describes the case of a 4-month-old boy with the cardinal symptoms of Crigler-Najjar syndrome type II. Molecular genetic analysis showed .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.5
- DOI:
- 10.4238/2013.September.4.5
Effect of muscle-fiber type on glycogenin-1 gene expression and its relationship with the glycolytic potential and pH of pork
Authors: S.-H. Zhang, L. Zhu, Z.-H. Wu, Y. Zhang, G.-Q. Tang, Y.-Z. Jiang, M.-Z. Li, L. Bai and X.-W. Li
This study analyzed the effect of muscle-fiber type composition on glycogenin-1 (GYG) gene expression and its impact on pH. The longissimus dorsi (LD) muscle contains more type IIB fibers (75.10%) than does the psoas major (PM) muscle (41.58%), while the PM has more type I (3.65 vs 0.94%), type IIA (34.15 vs 10.63%), and type IIX (20.62 vs 13.33%) fibers... Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.4
- DOI:
- 10.4238/2013.September.4.4
Association between a polymorphism of the �±-lactalbumin gene and milk production traits in Chinese Holstein cows
Authors: J.P. Zhou and C.H. Dong
The traits particularly important for milk production include milk yield, protein percentage, fat percentage, and the somatic cell score. Alpha-lactalbumin (α-LA) is an important whey protein of cow milk, and is also present in the milk of many other mammalian species. In this study, we analyzed the genetic polymorphisms of the α-LA gene and t.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.3
- DOI:
- 10.4238/2013.September.4.3
Construction of recombinant adenoviruses carrying the optimal shRNA template against goat PTHrP and successful suppression of PTHrP expression in mammary epithelial cells
Authors: H.L. Zheng, X.M. Liu, Q. Yu, R.F. Xing, Z.Y. Yang and J. Luo
Parathyroid hormone-related protein (PTHrP) is a protein member of the parathyroid hormone family that regulates the dynamic balance between blood and bone calcium during lactation. However, the mechanism of its regulation is not very clear. In order to establish a framework for further functional studies of the PTHrP gene in goat mammary gland epithelial.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.2
- DOI:
- 10.4238/2013.September.4.2
Association analysis of yield and fiber quality traits in Gossypium barbadense with SSRs and SRAPs
Authors: X.Q. Wang, Y. Yu, W. Li, H.L. Guo, Z.X. Lin and X.L. Zhang
Cotton is an important cash crop. Mining for quantitative trait loci related to yield and fiber quality traits using association analysis has many advantages for cotton research. In this study, 170 simple sequence repeats (SSRs) and 258 sequence-related amplified polymorphisms (SRAPs) were used to analyze the association of 3 yield component traits and 5 .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.1
- DOI:
- 10.4238/2013.September.4.1
Significance of TNF-�± and IL-6 expression in invasive pituitary adenomas
Authors: J.L. Wu, J.Y. Qiao and Q.H. Duan
The aim of this study was to investigate expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in invasive pituitary adenomas (IPAs). The streptavidin peroxidase immunohistochemical method was employed to detect their expression in 40 cases of IPA and 40 of non-invasive pituitary adenoma (NIPA). TNF-α and IL-6 were found.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017502
- DOI:
- 10.4238/gmr.15017502
Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia
Authors: A.S. Al-Wadai, M.R. Al-Othman, M.A. Mahmoud and A.R.M. Abd El-Aziz
Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.10
- DOI:
- 10.4238/2013.September.3.10